LOCUS       sequence-216402-       15136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK652
FEATURES             Location/Qualifiers
     source          1..15136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8346..8355
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8352..9083
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             9159..9488
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    9638..9719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9741..10251
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             10288..11307
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             10288..11307
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     polyA_signal    11621..11844
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12948..12968)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12948..12966)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12987..13003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12987..13003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13000..13022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13011..13027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13035..13041,13042..13059,13060..13065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13080..13101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13389..13977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13469..13488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14148..14939,14940..15008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14771..14790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15009..15113)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa tcggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     5341 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     5401 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     5461 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     5521 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     5581 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     5641 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     5701 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     5761 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     5821 ggcagcagaa cgaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     5881 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     5941 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     6001 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     6061 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     6121 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     6181 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     6241 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     6301 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     6361 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     6421 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     6481 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     6541 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaggcactg cgggcagagg
     6601 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     6661 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     6721 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     6781 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     6841 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     6901 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     6961 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     7021 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     7081 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     7141 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     7201 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     7261 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     7321 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     7381 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     7441 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     7501 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     7561 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     7621 tggaccaggc ccgggagaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     7681 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     7741 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     7801 agaggcagaa actcactgcc caggtggagc agctagaggt atttcagaga gagcaaacta
     7861 agcaggtgga agaactgagt aagaaactgg ctgactctga ccaagccagc aaggtgcagc
     7921 agcagaagct gaaggctgtc caggctcagg gaggcgagag ccagcaggag gcccagcgcc
     7981 tccaggccca gctgaatgaa ctgcaagccc agttgagcca gaaggagcag gcagctgagc
     8041 actataagct gcagatggag aaagccaaaa cacattatga tgccaagaag cagcagaacc
     8101 aagagctgca ggagcagctg cggagcctgg agcagctgca gaaggaaaac aaagagctgc
     8161 gagctgaagc tgaacggctg ggccatgagc tacagcaggc tgggctgaag accaaggagg
     8221 ctgaacagac ctgccgccac cttactgccc aggtgcgcag cctggaggca caggttgccc
     8281 atgcagacca gcagcttcga gacctgggca aattcggagg tggatctacg cgtggaggtg
     8341 gatctgccac catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc
     8401 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     8461 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
     8521 tcgccttcga catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca
     8581 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag
     8641 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
     8701 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga
     8761 tgcagaagaa aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     8821 gcctggaagg cagaaccgac atggccctga agctcgtggg cgggggccac ctgatctgca
     8881 acttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct
     8941 actatgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     9001 agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta
     9061 atggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaacgaat
     9121 tcggcattga tctgagcggc ctgaccctgc aggaattcag ctccccgaaa cgccctaagc
     9181 tgctgcgtga atattacgat tggctggttg ataacagctt taccccatat ctggtggtgg
     9241 atgccacata cctgggcgtg aacgtgcccg tggagtatgt gaaagacggt cagatcgtgc
     9301 tgaatctgtc tgcaagtgcg accggcaacc tgcaactgac aaatgatttt atccagttca
     9361 acgcccgctt taagggcgtg tctcgtgaac tgtatatccc gatgggtgcc gctctggcca
     9421 tttacgctcg cgagaacggc gatggtgtga tgttcgaacc agaagaaatc tatgacgagc
     9481 tgaatattgg ttaaaccggt ggatccagat ctctgcagca tatgtctaga ggatcataat
     9541 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     9601 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     9661 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgcc
     9721 gacgtcaggt ggcacttttc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     9781 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     9841 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     9901 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     9961 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
    10021 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
    10081 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
    10141 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
    10201 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca
    10261 ccggagctta ccatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag
    10321 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    10381 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    10441 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    10501 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    10561 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    10621 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    10681 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    10741 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    10801 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    10861 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10921 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    10981 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    11041 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    11101 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    11161 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    11221 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    11281 agcactcgtc cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga
    11341 gaacgcctca gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta
    11401 cgcttggtgg cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag
    11461 ggccggtcgc agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca
    11521 tgcccacgca ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg
    11581 ccgattgggt gcagatctag agctcgctga tcagcctcga ctgtgcctct agttgccagc
    11641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
    11701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
    11761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
    11821 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatga
    11881 gggagctgcg gtggaggagg tggagagaag gccgcaccct tctgggcagg gggaggggag
    11941 tgccgcaata cctttatggg agttctctgc tgcctcccgt cttgtaagga ccgccctggg
    12001 cctggaagaa gccctccctc ctttcctcct cgcgtgatct cgtcatcgcc tccatgtcga
    12061 gtcgcttctc gattatgggc gggattcttt tgcctaggct taaggggcta acttggtccc
    12121 tgggcgttgc cctgcagggg agtgagcagc tgtaagattt gaggggcgac tccgattagt
    12181 ttatcttccc acggactaga gttggtgtcg aggttattgt aataagggtg gggtagggaa
    12241 atggagctta gtcattcacc tggggctgat tttatgcaac gagactgcgg attatcacta
    12301 cttatcattt ttggagcatt tttctagaga cagacataaa gcatgatcac ctgagtttta
    12361 taccatttga gacccttgct gcaccaccaa agtgtagcat caggttaaat cttaatagaa
    12421 aaattttagc ttttgcttga gaaaccagtg cttccctccc tcaccctctc tccccaggct
    12481 ctctacccct ttgcatccct accaggcatc ttagcaactc tcactcatac ttgatcccat
    12541 tttccatttg ttgtacttgc tcctctagta ttcagacata gcactagctt tctccctctc
    12601 ttgatcttgg gtagcctggt gtctcgcgaa accagacaga ttggttccac cacaaattaa
    12661 ggcttgagct ggggcttgac tcttacccag cagtgctttt attcctccct agttcacgtt
    12721 cttaaatgtt tatcttgatt ttcattttat cctttttcct tagctgggat tctgtccctg
    12781 accgtcttca cagtccaggt gatcttgact actgctttac agagaattgg atctgaggtt
    12841 aggcaacatc tccctttttc ttcctctaaa tacctctcat ttctgttctt accagttagt
    12901 aactgatctc agatgcctgt gtgatagctg gtacccagct tttgttccct ttagtgaggg
    12961 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    13021 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    13081 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    13141 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    13201 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    13261 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    13321 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    13381 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    13441 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    13501 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    13561 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    13621 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    13681 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    13741 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    13801 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    13861 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    13921 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    13981 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    14041 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    14101 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    14161 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    14221 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    14281 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    14341 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    14401 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    14461 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    14521 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    14581 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    14641 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    14701 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    14761 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    14821 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    14881 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    14941 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    15001 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    15061 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    15121 ccccgaaaag tgccac
//
