LOCUS       sequence-216420-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); MCS and a 25nm
            GS linker are present downstream of BioID2 and myc tags..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux 
            KJ
  TITLE     An improved smaller biotin ligase for BioID proximity labeling.
  JOURNAL   Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
  PUBMED    26912792
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2016 Feb 24. pii: mbc.E15-12-0844."
COMMENT     Sequence Label: mycBioID2-13X Linker-MCS
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcggttta aacttaagct tggtaccgag
      121 ctcggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc
      181 tcgaggttct tgaaacggcg cgccgatcca ccgcctccgc tacctccgcc accacttcca
      241 ccgcctccgc tacctccgcc accgctacct ccgccaccag aacctcctcc accacttcca
      301 ccgcctccac ttccaccgcc tccgctacct ccgccacccg acccgcctcc acccccgctt
      361 ccacccccgc cactaccccc gcctccagac ccgcctccac cggatccgaa ttccgaaaaa
      421 ccggtgcttc ttctcaggct gaactcgccg ctcaggatct ccttgatgcc ctcctcggtc
      481 aggatcaggg cgccgccctt ctcgctcagg cccaccagct tgccggtgat cttgccctcg
      541 cccagcagct tcacctcctc gcccaggtac agcatcttgc tctcgatctt gcccttgaac
      601 tccttgaagc tcttctcctt gaacttcttc aggttctcgc tgattctctt cagcaccttc
      661 agcagcacct cctttctgtc ccagtccttg c
//
