LOCUS       sequence-216517-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Prey vector TMKL1 X013_pECIA14 should be used with bait vector TMKL1
            X013_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: TMKL1 X013_pECIA14
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     44..97
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             761..784
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 gatgggatat cctcagtgca ctaagggggg atccgatctc aatatgaagt tatgcatatt
       61 actggccgtc gtggcctttg ttggcctctc gctcgggtct ctctctggtt cttctgatgt
      121 gaagcttctt ttaggaaaaa tcaagtcttc actacaagga aacagtgaga gcttactgtt
      181 gtcttcttgg aactcctctg ttcctgtttg tcaatggaga ggtgtaaaat gggtattttc
      241 aaatgggtct cctcttcaat gtagtgacct ctcttcacca caatggacta atacctctct
      301 gttcaacgac tcttctcttc accttctctc tcttcagctt ccttctgcta atctcactgg
      361 ttcactccct agagagattg gtgagttctc tatgcttcaa agtgtgttcc tcaacatcaa
      421 ttcattaagt gggtcaatcc ctcttgagct tggttacact tcttctctct ctgatgttga
      481 tttgagtggt aatgccttag ctggggtttt gcctccatcg atttggaacc tctgtgataa
      541 gcttgtctct ttcaagattc atggtaataa cttgtctggg gttttgcctg agcctgcttt
      601 gccaaattcg acttgtggta atctccaagt tcttgatttg ggtggtaata agttctcaga
      661 ttttggtgag tcaaagtttg gagcagaatc tttcgaaggg aacagtccta gcctttgtgg
      721 tttgcctttg aagccttgtc taggctcctc taggttaagt ctggaggtgc tgttccaggg
      781 accaagcggc aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac
      841 caatgccgcc ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaaag gagatcacct
      901 tcctgaagaa caccgtgatg gagtgcgacg cctgcggcat gcagcctcta gaggctccgg
      961 ctccatcatc ccagttgagg aggagaaccc ggacttctga ccgcgaggca gccgaggtcc
     1021 ttgggtgccg caagagctgc agctgcacag aacagccgcc agaacctcat catctctggg
     1081 cgatgggatg gggggtgtcc ttacggtgac agctggcagg gatcctaaaa gcccg
//
