LOCUS       sequence-21663-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Dyer MA, Cepko CL
  TITLE     p27Kip1 and p57Kip2 regulate proliferation in distinct retinal 
            progenitor cell populations.
  JOURNAL   J Neurosci. 2001 Jun 15. 21(12):4259-71.
  PUBMED    11404411
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2001 Jun 15. 21(12):4259-71."
COMMENT     Sequence Label: pLIA-E p27 (CC#620)
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             736..753
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcggccggcc gcataggcca ctagtggatc ccagtgtggt ggtacgtagg aattccggtc
       61 gagcagctga agcttgcatg cctgcaggtc gactctagaa ccatggacta caaggacgac
      121 gatgacaagg cggccgcatc aaacgtgaga gtgtctaacg ggagcccgag cctggagcgg
      181 atggacgcca gacaagcgga tcaccccaag ccttccgcct gcagaaatct cttcggcccg
      241 gtcaatcatg aagaactaac ccgggacttg gagaagcact gccgggatat ggaagaagcg
      301 agtcagcgca agtggaattt cgactttcag aatcataagc ccctggaggg cagatacgaa
      361 tggcaggagg tggagagggg cagcttgccc gagttctact acaggccccc gcgccccccc
      421 aagagcgcct gcaaggtgct ggcgcaggag agccaggatg tcagcgggag ccgccaggcg
      481 gtgcctttaa ttgggtctca ggcaaactct gaggaccggc atttggtgga ccaaatgcct
      541 gactcgtcag acaatcaggc tgggttagcg gagcagtgtc cagggatgag gaagcgacct
      601 gctgcagaag attcttcttc gcaaaacaaa agggccaaca gaacagaaga aaatgtttca
      661 gacggttccc cgaacgctgg cactgtggag cagacgccca agaagcccgg ccttcgacgc
      721 cagacggcgg ccgcacatca tcatcatcat cattaatcta gaggatc
//
