LOCUS       sequence-21678-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Slimko EM, Lester HA
  TITLE     Codon optimization of Caenorhabditis elegans GluCl ion channel genes
            for mammalian cells dramatically improves expression levels.
  JOURNAL   J Neurosci Methods. 2003 Mar 30. 124(1):75-81.
  PUBMED    12648766
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods. 2003 Mar 30. 124(1):75-81."
COMMENT     Sequence Label: optGluCl alpha EYFP
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc ttggtaccga gctcggatcc actagtccag
      121 tgtggtggaa ttgcccttgc caccatggcc acgtggatcg tgggcaagct gatcatcgct
      181 tccctgatcc tggggatcca ggcccagcag gcccgcacca agagccagga catcttcgag
      241 gacgacaacg acaacggcac taccaccctg gagtccctgg ctcgcctgac ctcccccatc
      301 cacatcccca tcgagcagcc ccagacctcc gacagcaaga tcctggccca cctgttcacc
      361 agcggctacg acttccgcgt gcgccccccc accgacaacg gcggccccgt ggtggtgagc
      421 gtgaacatgc tgctgcgcac catcagcaag atcgacgtgg tgaacatgga gtactccgcc
      481 cagctgaccc tgcgcgagag ctggatcgac aagcgcctgt cctacggcgt caagggcgac
      541 ggccagcccg acttcgtcat cctgaccgtg ggccaccaga tctggatgcc cgacaccttc
      601 ttccccaacg agaagcaggc ttacaagcac accatcgaca agcccaacgt cctgatccgc
      661 atccacaacg acggcaccgt cctgtactcc gtccgcatca gcctggtgct gagctgcccc
      721 atgtacctgc agtactaccc gatggacgtg cagcagtgca gcatcgacct ggc
//
