LOCUS       sequence-21679-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Slimko EM, Lester HA
  TITLE     Codon optimization of Caenorhabditis elegans GluCl ion channel genes
            for mammalian cells dramatically improves expression levels.
  JOURNAL   J Neurosci Methods. 2003 Mar 30. 124(1):75-81.
  PUBMED    12648766
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods. 2003 Mar 30. 124(1):75-81."
COMMENT     Sequence Label: optGluCl beta Y182F ECFP
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agctctctgg ctaatagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagttaagc ttggtaccga gctcggatcc actagtccag
      121 tgtggtggaa ttgcccttgc caccatggcc accccctcca gcttctcgat cctgctgctg
      181 ctgctgctga tgcccgtggt gaccaacggc gagtacagca tgcagagcga gcaggagatc
      241 ctgaatgccc tgctgaagaa ctacgacatg cgcgtgcgcc ccccccccgc caacagcagc
      301 accgagggcg ccgtgaacgt gagagtgaac atcatgatca gaatgctgag caagatcgac
      361 gtggtgaaca tggagtactc catccagctg accttccgcg agcagtggat cgacccccgc
      421 ctggcctacg agaacctggg cttctacaac ccccccgcct tcctgaccgt gccccacgtc
      481 aagaagtccc tgtggatacc cgacaccttc ttccccaccg agaaggccgc ccaccgccac
      541 ctgatcgaca tggagaacat gttcctgcgc atctaccccg acggcaagat cctgtacagc
      601 agccgcatca gcctgacgag ctcctgcccc atgcgcctgc agctgtaccc cctggactac
      661 cagagctgca acttcgacct ggtgtccttc gcccacacca tgaacgacat catgtatgag
      721 tgggacccca gcacccccgt g
//
