LOCUS       sequence-21681-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Nashmi R, Dickinson ME, McKinney S, Jareb M, Labarca C, Fraser SE, 
            Lester HA
  TITLE     Assembly of alpha4beta2 nicotinic acetylcholine receptors assessed 
            with functional fluorescently labeled subunits: effects of 
            localization, trafficking, and nicotine-induced upregulation in 
            clonal mammalian cells and in cultured midbrain neurons.
  JOURNAL   J Neurosci. 2003 Dec 17. 23(37):11554-67.
  PUBMED    14684858
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2003 Dec 17. 23(37):11554-67."
COMMENT     Sequence Label: nAChR beta2 YFP
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..245
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(291..317)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
       61 tcggggtagc gggcgaagca ctgcaggccg tagccgaagg tggtcacgag ggtgggccag
      121 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      181 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      241 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggcgtagtct
      301 gggacgtcat aaggataagg acctttgcgg aagaatagtg tgcctgcccc ctcacgttcc
      361 cgctggcgcc ttcgcaagcg caggcgttga cgtgcacagc ggtggcgtgg ctgctgcagg
      421 aagaggaggg tgggcagctt ctccaggaag accaccttga cccagggcgc catggtgtgc
      481 gtggtaggcg aacggtggtg cacattgagc acacacacgc tagtgacgat ggagaaggtg
      541 actagcacca tggtgaacat gaggtacttt cccaccagcg gtacgtcgag ggaggtggga
      601 ggcacaatct tggagatgag cagcaggaac accgtgagcg ccagcagcac agaaatacaa
      661 agtgtcatct tttcaccaca gtctgagggc aggtagaaga ccaggatggc cagcgaggtg
      721 atgagtacgc aggggatgat gaggttgatg gtgt
//
