LOCUS       sequence-21682-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Harpavat S, Cepko CL
  TITLE     RCAS-RNAi: a loss-of-function method for the developing chick 
            retina.
  JOURNAL   BMC Dev Biol. 2006 Jan 22;6:2. doi: 10.1186/1471-213X-6-2.
  PUBMED    16426460
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2006-01-22"; volume: "6:2. doi"; pages: " 
            10.1186/1471-213X-6-2"
COMMENT     Sequence Label: pCS6/U6
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     73..89
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        86..399
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     318..338
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     411..427
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     412..428
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..478)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(515..535)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(515..533)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(574..598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(600..618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(629..645)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..660)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tggtaccggt cgtaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
       61 ttgggtaccc gctctagaac tagtggatcc gacgccgcca tctctaggcc cgcgccggcc
      121 ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat
      181 aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt
      241 ttaatactag ctacatttta catgataggc ttggatttct ataagagata caaatactaa
      301 attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg
      361 ttttgagact ataaatatcc cttggagaaa agccttgttt gggccccccc tcgaggtcga
      421 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
      481 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct
      541 tggcgtaatc atggtcatag ctgtttcccg cgtacccagc tttcttgtac aaagtggtcc
      601 ctatagtgag tcgtattata agctaggcac tggccgtcgt tttacaacgt cgtgactggg
      661 aaactgctag cttgggatct ttgtgaggaa ccttacttct gtggtgtgac ataattggac
      721 aaactaccta cag
//
