LOCUS       sequence-21683-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Harpavat S, Cepko CL
  TITLE     RCAS-RNAi: a loss-of-function method for the developing chick 
            retina.
  JOURNAL   BMC Dev Biol. 2006 Jan 22;6:2. doi: 10.1186/1471-213X-6-2.
  PUBMED    16426460
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2006-01-22"; volume: "6:2. doi"; pages: " 
            10.1186/1471-213X-6-2"
COMMENT     Sequence Label: G-RCAS (A)
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..223
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(248..253,254..271,272..278)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(398..417)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gacagctatt ggtaattgtg aaatacgctt ttgtctgtgt gctgcaggag ctgagctgac
       61 tctgctggtg gcctcgcgta ccactgtggc atcgatcaac aagtttgtac aaaaaagctg
      121 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      181 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac
      241 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc
      301 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
      361 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
      421 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
      481 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
      541 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
      601 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
      661 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
      721 cctggcctat ttccctaaag ggtttattg
//
