LOCUS       sequence-21684-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p404MET25
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     302..318
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     302..318
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(352..368)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(353..369)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      371..618
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(387..405)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(636..655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(637..655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcacgttgaa gctgtcgata ttggggaacc gtggtggttg gcaaatgact aattaagtta
       61 gtcaaggcgc catcctcatg aaaactgtgt aacataataa ccgaagtgtc gaaaaggtgg
      121 caccttgtcc aattgaacac gctcgatgaa aaaaataaga tatatataag gttaagtaaa
      181 gcgtctgtta gaaaggaagt ttttcctttt tcttgctctc ttgtcttttc atctactatt
      241 tccttcgtgt aatacagggt cgtcagatac atagatacaa ttctattacc cccatccata
      301 ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
      361 cgacctcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
      421 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
      481 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
      541 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
      601 tcgaaggctt tattttgcgg ccggtaccca attcgcccta tagtgagtcg tattacgcgc
      661 gctcactggc cgtcgtttt
//
