LOCUS       sequence-21685-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Rizzo MA, Springer GH, Granada B, Piston DW
  TITLE     An improved cyan fluorescent protein variant useful for FRET.
  JOURNAL   Nat Biotechnol. 2004 Apr . 22(4):445-9.
  PUBMED    14990965
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2004 Apr . 22(4):445-9."
COMMENT     Sequence Label: Cerulean
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     225..246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      288..297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(336..539)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(365..385)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cnagggctct ggagtgcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
       61 gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga gggtgggcca
      121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
      181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
      241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
      301 cggtagcgct agcggatctg acggttcact aaaccagctc tgcttatata gacctcccac
      361 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      421 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
      481 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
      541 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
      601 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
      661 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
      721 cattgacgtc aatggaaagt ccctattggc gttactatga aaanatacnt cntnttgact
      781 catgggcggg gtcttgggcg tcaccag
//
