LOCUS       sequence-216891-        6164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration vector for phiC31-mediated transgenesis using a genomic 
            attP-CC site. Contains an attB-CC site, the visible GMR-white 
            flanked by a FRT site for FLP-mediated removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Blanco-Redondo B, Langenhan T
  TITLE     Parallel Genomic Engineering of Two Drosophila Genes Using 
            Orthogonal attB/attP Sites.
  JOURNAL   G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565.
  PUBMED    30065043
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565."
COMMENT     Sequence Label: pGE-attBCC-FRT-mW (pTL788)
FEATURES             Location/Qualifiers
     source          1..6164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    455..488
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(3536..4090,4091..4121)
                     /label=P element 5' end
     rep_origin      complement(4356..4944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4436..4455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5115..5906,5907..5975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5738..5757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5976..6080)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagctag
      241 agttgttggt tggcacacca caaatatact gttgccgagc acaattccgc tcgaattgcc
      301 ggctgtacac ttaagaagtt cctactgcag gtaatgaatt gccgcggtgc gggtgccagg
      361 gcgtgccccc gggctccccg ggcgcgtact ccacgaattc ttgcatgcaa tgcggccgct
      421 agcagatcta ctagtctcga ggcgcgccgg taccgaagtt cctattctct agaaagtata
      481 ggaacttccg tacgactagc acctgactgt cgagcccagt ggaaaccctt gaaatgcctt
      541 taagtcgagc ccagtggaaa cccttgaaat gcctttaagt cgagcccagt ggaaaccctt
      601 gaaatgcctt taagtcgagc ccagtggaaa cccttgaaat gcctttaagt cgagcccagt
      661 ggaaaccctt gaaatgcctt taagtcgagt agcagtcgag gtaggtacgc catgccccgt
      721 tattctctat tcgttttgtg actctccctc tctgtactat tgctctctca ctctgtcgca
      781 cagtaaacgg cacgctattc tcgttgcttc gagagagcgc gcctcgaatg ttcgcgaaaa
      841 gagcgccgga gtataaatag aggcgcttcg tcgacggagc gtcaattcaa ttcaaacaag
      901 caaagtgaac acatcgcgaa gcgtaagctg agcaaacaaa caagcgcagc tgaacaagct
      961 aaacaatctg caataaagtg caagttaaag tgaatcaatt aaaagtaacc aacaaccaag
     1021 taattaaact aaaaactgca actactgaaa tcaaccaaga agtcattatt gaagacaaga
     1081 agagaactct gaataggtcg atagcgtcaa ttcggcacga gcggtttcgt gacgaagctc
     1141 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
     1201 atttttgttt gttttttgag tgattgggtg ggtgattggt tttgggtggg taagcagggg
     1261 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
     1321 agcaaacacc catctgccga gcatctgaac aatggtgaca gcggagcggc ttcgcagagc
     1381 tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctcctgcc acccagtccg
     1441 ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac
     1501 aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac
     1561 cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc
     1621 aagaacgttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
     1681 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
     1741 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
     1801 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
     1861 ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
     1921 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
     1981 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
     2041 gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactca
     2101 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
     2161 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
     2221 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
     2281 tacgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     2341 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     2401 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatttggag
     2461 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     2521 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     2581 cgacttattc agacaacgat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     2641 ctcacgcaag tgggcgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     2701 acctttcaaa acgtctttgc cacgataaat gtgttcacct cagagctgcc agtttttatg
     2761 agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa aacgattgcc
     2821 gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta tccgatgatc
     2881 ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac tctggtggcc
     2941 aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac ctcgatggcg
     3001 ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt cttcttgaac
     3061 tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc
     3121 aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca
     3181 tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc
     3241 gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag cttccgggtg
     3301 ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac atatatccga
     3361 aataactgtt tgtttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc
     3421 ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata
     3481 caaaaaaaaa aaaaaaaaaa aaaaaaaaac tcgacaccgg gatccaaacc tagtggctgc
     3541 tgctctaaac gacgcatttc gtactccaaa gtacgaattt tttccctcaa gctcttattt
     3601 tcattaaaca atgaacagga cctaacgcac agtcacgtta ttgtttacat aaatgatttt
     3661 ttttactatt caaacttact ctgtttgtgt actcccactg gtatagcctt cttttatctt
     3721 ttctggttca ggctctatca ctttactagg tacggcatct gcgttgagtc gcctcctttt
     3781 aaatgtctga ccttttgcag gtgcagcctt ccactgcgaa tcattaaagt gggtatcaca
     3841 aatttgggag ttttcaccaa ggctgcaccc aaggctctgc tcccacaatt ttctcttaat
     3901 agcacacttc ggcacgtgaa ttaattttac tccagtcaca gctttgcagc aaaatttgca
     3961 atatttcatt tttttttatt ccacgtaagg gttaatgttt tcaaaaaaaa attcgtccgc
     4021 acacaacctt tcctctcaac aagcaaacgt gcactgaatt taagtgtata cttcggtaag
     4081 cttcggctat cgacgggacc accttatgtt atttcatcat gggccagacc cacgtagtcc
     4141 agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc cgaactgggg
     4201 cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc ccattggtta
     4261 atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca tttgaggtat
     4321 actggcaccg agcccgagtt caagaagaag gcgtttttcc ataggctccg cccccctgac
     4381 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4441 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4501 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4561 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4621 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4681 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4741 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4801 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4861 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6001 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6061 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcgaa gtcttagagc
     6121 cagatatgcg agcacccgga agctcacgat gagaatggcc agac
//
