LOCUS       sequence-216892-        5978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for generating dsDNA donors for HDR. Contains an attP-CC 
            docking site, the visible GMR-white for identifying backbone 
            incorporation flanked by FRT sites for FLP-mediated removal..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Blanco-Redondo B, Langenhan T
  TITLE     Parallel Genomic Engineering of Two Drosophila Genes Using 
            Orthogonal attB/attP Sites.
  JOURNAL   G3 (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565.
  PUBMED    30065043
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2018 Jul 31. pii: g3.118.200565. doi: 
            10.1534/g3.118.200565."
COMMENT     Sequence Label: pHD-mW-attPCC-FRT (pTL717)
FEATURES             Location/Qualifiers
     source          1..5978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(860..951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     993..1012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1018..1037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1339..1372
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    4410..4443
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4979..4996)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4979..4995)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5094..5111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5265..5853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5345..5364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5977..5978,1..790,791..859))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg ctgaagcagg tggaattctt gcatgctagc
     1261 ggccgcggac atatgcacac ctgcgatcgt agtgccccaa ctggggtaac ctccgagttc
     1321 tctcagttgg gggcgtagga agttcctatt ctctagaaag tataggaact tccgtacgac
     1381 tagcacctga ctgtcgagcc cagtggaaac ccttgaaatg cctttaagtc gagcccagtg
     1441 gaaacccttg aaatgccttt aagtcgagcc cagtggaaac ccttgaaatg cctttaagtc
     1501 gagcccagtg gaaacccttg aaatgccttt aagtcgagcc cagtggaaac ccttgaaatg
     1561 cctttaagtc gagtagcagt cgaggtaggt acgccatgcc ccgttattct ctattcgttt
     1621 tgtgactctc cctctctgta ctattgctct ctcactctgt cgcacagtaa acggcacgct
     1681 attctcgttg cttcgagaga gcgcgcctcg aatgttcgcg aaaagagcgc cggagtataa
     1741 atagaggcgc ttcgtcgacg gagcgtcaat tcaattcaaa caagcaaagt gaacacatcg
     1801 cgaagcgtaa gctgagcaaa caaacaagcg cagctgaaca agctaaacaa tctgcaataa
     1861 agtgcaagtt aaagtgaatc aattaaaagt aaccaacaac caagtaatta aactaaaaac
     1921 tgcaactact gaaatcaacc aagaagtcat tattgaagac aagaagagaa ctctgaatag
     1981 gtcgatagcg tcaattcggc acgagcggtt tcgtgacgaa gctccaagcg gtttacgcca
     2041 tcaattaaac acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt
     2101 tgagtgattg ggtgggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc
     2161 cggcaatggg ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg
     2221 ccgagcatct gaacaatggt gacagcggag cggcttcgca gagctgcatt aaccagggct
     2281 tcgggcaggc caaaaactac ggcacgctcc tgccacccag tccgccggag gactccggtt
     2341 cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg gacatctttg
     2401 gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca cgcggactat
     2461 tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac gtttgcggcg
     2521 tggcctatcc gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc
     2581 tgctgaatgc ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc
     2641 gactgctcaa tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc
     2701 agcaggatga cctctttatc ggctccctaa cggccaggga acacctgatt ttccaggcca
     2761 tggtgcggat gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga
     2821 tccaggagct ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga
     2881 aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc ctccgaggca ctaaccgatc
     2941 cgccgcttct gatctgcgat gagcccacct ccggactgga ctcatttacc gcccacagcg
     3001 tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc
     3061 agccgtcttc cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg
     3121 tagctttctt gggcactccc agcgaagccg tcgacttctt ttcctacgtg ggtgcccagt
     3181 gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac
     3241 gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc attagcaaag
     3301 tagcccggga tatggagcag ttgttggcca ccaaaaattt ggagaagcca ctggagcagc
     3361 cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc
     3421 gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa
     3481 cgatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggcg
     3541 tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct
     3601 ttgccacgat aaatgtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     3661 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     3721 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     3781 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     3841 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     3901 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     3961 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     4021 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     4081 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     4141 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     4201 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgtttgtttt
     4261 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     4321 aattatattt gtgccaataa aaacaagata tgacctatag aatacaaaaa aaaaaaaaaa
     4381 aaaaaaaaaa aaactcgaca ccgggatccg aagttcctat tctctagaaa gtataggaac
     4441 ttcagaagag cactagtaaa gatctccatg cataaggcgc gcctaggcct tctgcagctc
     4501 gaggctcttc cgtcaatcga gttcaagggc gacacaaaat ttattctaaa tgcataataa
     4561 atactgataa catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt
     4621 tgatatcaaa aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt
     4681 aacaaactag aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta
     4741 taggtgttca tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca
     4801 tttataaggt taaataattc tcatatatca agcaaagtga caggcgccct taaatattct
     4861 gacaaatgct ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg
     4921 ttatttgcgg attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac
     4981 tggccgtcgt tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     5041 ggccttctgc ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc
     5101 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5161 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5221 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5281 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5341 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5401 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5461 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5521 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5581 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5641 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt gggctaacta cggctacact
     5701 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5761 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5821 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5881 tctgacgctc agtggaacga cgcgcgcgta actcacgtta agggattttg gtcatgagct
     5941 tgcgccgtcc cgtcaagtca gcgtaatgct ctgctttt
//
