LOCUS       sequence-216895-        2936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non binary level 1 position 2 vector adapted for MoClo modular 
            cloning useful for particle bombardment transformation of plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2936)
  TITLE     Non binary MoClo adapted vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MoClo adapted pUC57-Amp Level 1 position 2
FEATURES             Location/Qualifiers
     source          1..2936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             203..526
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    220..276
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(277..294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(277..293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(286..308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(502..521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    661..682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(799..816)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(970..1558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1050..1069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1729..2520,2521..2589))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2352..2371
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2590..2694)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2762..2780
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2818..2840)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtgaagacaa gcaagaattc aagcttggag tgagaccgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc
      241 gactctagag gatccccggg taccgagctc gaattcactg gccgtcgttt tacaacgtcg
      301 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      361 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      421 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      481 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      541 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      601 cagacaagct gtgacggtct cacgctacta ttgtcttctg cacgaagtgt ggggtgccta
      661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      721 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      781 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      841 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggggtaacgc
      901 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      961 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1021 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1081 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1141 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1201 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1261 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1321 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1381 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1441 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1501 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1561 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1621 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1681 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1741 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1801 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1861 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1921 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1981 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2041 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2101 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2161 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2221 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2281 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2341 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2401 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2461 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2521 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2581 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     2641 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2701 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2761 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     2821 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     2881 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc cactct
//
