LOCUS       sequence-216906-        8142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET28-derived plasmid that expresses an N-terminal maltose binding 
            fusion construct of CreM with E352A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Waldman AJ, Balskus EP
  TITLE     Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis.
  JOURNAL   J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367.
  PUBMED    29771512
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Org Chem.
            2018 May 29. doi: 10.1021/acs.joc.8b00367."
COMMENT     Sequence Label: pET-MBP-CreM_E352A
FEATURES             Location/Qualifiers
     source          1..8142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     559..578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(598..675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     881..900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     984..1003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        984..1002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1003..1027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1042..1064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1083..1100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1110..1127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1131..2231
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2088..2097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2205..2228
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2244..2261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2277..2294
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2295..2315
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3990..4007
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4060..4078)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4074..4121
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4158..4613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4245..4264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4455..4476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4705..5520)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4705..5520)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5428..5447
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5512..5531
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      5642..6230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6131..6150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6384..6401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6416..6555
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6481..6500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6641..6663
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6657..6848)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(7657..8142,1..597))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
       61 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
      121 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
      181 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
      241 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
      301 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
      361 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
      421 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
      481 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
      541 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
      601 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
      661 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
      721 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
      781 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
      841 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
      901 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      961 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
     1021 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca
     1081 gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgaaaatcg
     1141 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     1201 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     1261 tggaagagaa attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     1321 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     1381 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     1441 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     1501 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     1561 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     1621 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     1681 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     1741 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     1801 cgatgaccat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     1861 gtgtaacggt actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga
     1921 gcgcaggtat taacgccgcc agtccgaaca aagagctggc gaaagagttc ctcgaaaact
     1981 atctgctgac tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     2041 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgccgcc accatggaaa
     2101 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     2161 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     2221 acgcgcagac taattcgagc tcccaccatc accatcacca cgcgaattcg gtaccgctgg
     2281 ttccgcgtgg atctgagaac ctgtacttcc aatccggatc cgtgcacgca ctgctgcgcc
     2341 gggtcacacg cgggaacggc ttctacatcg gcgacctctt ccacgccgcc gccggccacg
     2401 acgccaccac accggtcacg ctcgaccagc cccttcagta cgcccccgag ctcggcacgc
     2461 gcttcaccgt cgggcagctc gccgagcaga cggacgaact ggccgcccgg ctctgggccg
     2521 cgggcgtgcg gcccaccgaa cgggtcgcgc tgtacaagaa ggacaacttc gacatcgcgc
     2581 tgcacgcctg cgccgccgcg cggatcggcg cggtccccgc cctcctgtcg cccgccctgg
     2641 aggccccggt cgtacggacg ctgctcgacc ggctcgacgg gccgtggctg ctgaccgacg
     2701 cgacgaccct cgccggcccc ctggggacgg tgctcgcacc ggcatccgta cgagcggtcc
     2761 tgctgacgac cggcacaccg gcggagggcg cgcttcccgc agccggcgcg accggacccg
     2821 ctcgcgaagg ggacgcgccg ccgcccgctc ccgtcgtgcg gctggccgac caccggggcg
     2881 ccccgaagcg gccgcccgtc ttcctgcacc cgcgccagcc ctcgctgatc acgcacagct
     2941 cgggaaccac cggcgtcccc aagctggcgg tgcactgccc cgaggcgggc tggcaccggc
     3001 tggttccgca gaaggtcgtg tcctggccga tccgcggcaa ggagaaggcg gcgctgtgca
     3061 tgacgttcgt gcactcgcgc ttctaccagg gcctggccat gttcctcagc cacggcaacc
     3121 cgatgctgat cgccgtcgac cccgacccga gccggatcgg cccgctgttc gcgcgcgaac
     3181 ggcccggcta catcgagacc caccccaaca cctacgtcga ctgggaagcg ctggccgacg
     3241 ccccgggcga accgctcgcc ggcgtacggg tcttcggtgc gaccttcgac gcgatgcacc
     3301 cccggaccat ccagcgcatg ctcggcgcct cccggcgcac ccgcccgctg ttcgtccagt
     3361 tctacggcca gtcggcgatc ggcccgatgg ccggccgctg gtacacccgg cgctcggcgg
     3421 ccaggatgaa cggccgctgc gtggggctgc cgctgcccgg cttcatctcg ctgcgggtgg
     3481 tcgacgacgc cgggaagcgg ctgcgcggcg ggcgcaccgg ccacctcgaa gtgcgcagca
     3541 ggacgcgcat cctcacctac ctcggcgagg accagcggta cgcggagcaa ttgcacgacg
     3601 gatggtggcg ggtgggcgac atgggccgcc gcgaccgctg gggcctgctg cacctgctgg
     3661 accgcgaggt cgaccggatc ggcgacctgg agagcagcct ggcggtggag gacctgctga
     3721 tgagccgcct ggaggaactg cgcgaggtgg tcctggtgcc cggggcggac ggcgaaccgg
     3781 tccccgtcgt cgccaccgtc gacgagagcc cgctcgacgc ggcgcgctgg cagcgggcga
     3841 ccagcgacct gccgacgatg gcgccggtgc gccagttccg gttcgaagac ctgccgcgca
     3901 cgtccacccg caagatccag aaaccggaac tggcccggct gatccagggc gtccgggcaa
     3961 ccgaccaggg agtgggggca tgactcgagc accaccacca ccaccactga gatccggctg
     4021 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     4081 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     4141 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4201 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4261 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4321 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4381 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4441 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4501 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4561 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4681 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
     4741 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     4801 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     4861 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     4921 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     4981 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     5041 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     5101 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     5161 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     5221 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     5281 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     5341 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     5401 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     5461 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     5521 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc
     5581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5761 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     6421 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     6481 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     6541 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6601 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6661 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     6721 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     6781 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     6841 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     6901 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     6961 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     7021 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     7081 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     7141 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     7201 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     7261 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc
     7321 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
     7381 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
     7441 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
     7501 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
     7561 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     7621 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7681 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7741 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     7801 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     7861 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     7921 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     7981 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     8041 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     8101 tatcggctga atttgattgc gagtgagata tttatgccag cc
//
