LOCUS       sequence-21699-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF,
            Horwitz AR
  TITLE     Paxillin phosphorylation sites mapped by mass spectrometry.
  JOURNAL   J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9.
  PUBMED    16254239
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2005 Nov 1. 118(Pt 21):4925-9."
COMMENT     Sequence Label: Flag-Paxillin
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cntggngcnt tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agctgccacc atggactaca aggacgacga
      121 tgacaaggga cctaagcttg gtaccgagct cggatccggc accatggacg acctcgatgc
      181 cttactggca gacctggaat ccaccacctc gcatatctct aagcgaccgg tgtttctaac
      241 agaggaaacg ccttactcct acccaactgg aaaccatacg taccaggaga ttgctgtgcc
      301 accgccggtg cccccgccgc cttccagtga ggccctaaat ggcactgtga ttgacccctt
      361 agaccagtgg caacccagcg tgtccagata cggccaccag caacctcagt cccagtctcc
      421 gatatacagc tctagtgcca aaagctccag tgcctctgtt cctagagacg ggctcagctc
      481 cccttctccc cgtgccagtg aggaggaaca cgtgtacagc ttcccaaaca agcagaagtc
      541 tgcagaacca tctcccacaa tgaccagcac ctccctgggc agcaacctct cggaactgga
      601 caggcttctc ctggaactga atgctgttca acataatccc cccagtggct tctcagcaga
      661 tgaagtcagc agaagcccat cactgcccaa tgtgaccgga cctcactatg tcatcccaga
      721 gagca
//
