LOCUS       sequence-217004-        1292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against rodent PlexinB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   McDermott JE, Goldblatt D, Paradis S
  TITLE     Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and 
            glutamatergic synapse development in the mammalian hippocampus.
  JOURNAL   Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 
            10.1016/j.mcn.2018.06.008.
  PUBMED    29981480
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2018-07-4"; volume: "92:50-66. doi"; pages: " 
            10.1016/j.mcn.2018.06.008"
COMMENT     Sequence Label: pSuper-PlexinB2
FEATURES             Location/Qualifiers
     source          1..1292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     158..175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    292..313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(328..333,334..351,352..358)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    366..382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     371..393
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     390..406
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     390..406
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        427..445
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     473..489
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     474..490
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(563..777)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(623..642)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(797..813)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(797..813)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(845..864)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(846..864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(871..888)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(871..887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(880..902)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1116..1135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
       61 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      121 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      181 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      241 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      301 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      361 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      421 gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgaggtc
      481 gacggtatcg ataagctttt ccaaaaagca agtccttcct tatcaatctc ttgaattgat
      541 aaggaaggac ttgcggggat ctgtggtctc atacagaact tataagattc ccaaatccaa
      601 agacatttca cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg
      661 cgccactccc ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc
      721 ccgcctagtg acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa
      781 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccaa
      841 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      901 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      961 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1021 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1081 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1141 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1201 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1261 tagtgggcca tcgccctgat agacggtttt tc
//
