LOCUS       sequence-217005-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA against rodent Sema4A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   McDermott JE, Goldblatt D, Paradis S
  TITLE     Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and 
            glutamatergic synapse development in the mammalian hippocampus.
  JOURNAL   Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 
            10.1016/j.mcn.2018.06.008.
  PUBMED    29981480
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2018-07-4"; volume: "92:50-66. doi"; pages: " 
            10.1016/j.mcn.2018.06.008"
COMMENT     Sequence Label: pSuper-Sema4A 42-3n
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     210..227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     211..227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     234..253
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        234..252
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     285..301
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     285..301
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        321..535
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     456..475
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(608..624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(653..671)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(705..727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    716..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(740..746,747..764,765..770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    785..806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(923..940)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
       61 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      121 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      181 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
      241 actcactata gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat
      301 cccccgggct gcaggaattc gaacgctgac gtcatcaacc cgctccaagg aatcgcgggc
      361 ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc tggcaggaag atggctgtga
      421 gggacagggg agtggcgccc tgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
      481 ataaacgtga aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc
      541 cccacaaagc cttgaccttc atttcaagag aatgaaggtc aaggctttgt tttttggaaa
      601 agcttatcga taccgtcgac ctcgaggggg ggcccggtac cagcttttgt tccctttagt
      661 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1081 cgttgctggc gtttttccat
//
