LOCUS       sequence-217006-        3448 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses membrane-targeted Sema4A with N-terminal myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   McDermott JE, Goldblatt D, Paradis S
  TITLE     Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and 
            glutamatergic synapse development in the mammalian hippocampus.
  JOURNAL   Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 
            10.1016/j.mcn.2018.06.008.
  PUBMED    29981480
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2018-07-4"; volume: "92:50-66. doi"; pages: " 
            10.1016/j.mcn.2018.06.008"
COMMENT     Sequence Label: pCMV-myc-Sema4A
FEATURES             Location/Qualifiers
     source          1..3448
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          125..221
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      282..291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2654..2788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2678..2697)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2732..2751
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2899..2915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2899..2915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2912..2934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2923..2939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2947..2953,2954..2971,2972..2977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2992..3013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3130..3147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3381..3400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
       61 gatccagcct ccggactcta gaggatccgg tactagagga actgaaaaac cagaaagtta
      121 actggtaagt ttagtctttt tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa
      181 tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac
      241 ttctgctcta aaagctgcgg aattgtaccc gcgggcccgc cgccaccatg gctgggattt
      301 tctatttcat cctcttttcg tttctctttg gaatttgcga cgctgtcacc ggttctagag
      361 ggcccaccat ggcatcaatg cagaagctga tctcagagga ggacctgctt atggccatgg
      421 aggcccgaac tggtggtcag gggcccatgc ccagagtcaa ataccatgct ggagacgggc
      481 gcagggccct cagcttcttc caacaaaaag gcctccgaga ctttgacacg ctgctcctga
      541 gtgacgatgg caacactctc tatgtggggg ctcgagaggc cgtcctggcc ttgaatatcc
      601 agaacccagg aatcccaagg ctaaagaaca tgataccctg gccagccagt gagagaaaaa
      661 agaccgaatg tgcctttaag aagaagagca atgagacaca gtgtttcaac ttcattcgag
      721 tcctggtctc ttacaatgct actcacctct atgcctgtgg gacctttgcc ttcagccctg
      781 cctgtacctt cattgaactc caagattccc tcctgttgcc catcttgata gacaaggtca
      841 tggacgggaa gggccaaagc ccctttgacc ctgttcacaa gcacacagct gtcttggtcg
      901 atgggatgct ttattccggc accatgaaca acttcctggg cagcgagccc atcctgatgc
      961 ggacactggg atcccagcct gttctcaaga ctgacatctt cttacgctgg ctgcacgcgg
     1021 atgcctcctt cgtggcagcc attccatcca cccaggtcgt ctatttcttc tttgaggaga
     1081 cagccagcga gtttgacttc tttgaagagc tgtatatatc cagggtggct caagtctgca
     1141 agaacgacgt gggcggtgaa aagctactgc agaagaagtg gaccaccttc ctcaaagccc
     1201 agttgctctg cgctcagcca gggcagctgc cattcaacat catccgccac gcggtcctgc
     1261 tgcctgccga ttctccctct gtttcccgca tctacgcagt ctttacctcc cagtggcagg
     1321 ttggcgggac caggagctca gcagtctgtg ccttctctct cacggacatt gagcgagtct
     1381 ttaaagggaa gtacaaggag ctgaacaagg agacctcccg ctggaccact tgccggggct
     1441 cagaggtcag cccgaggcca ggcagttgct ccatgggccc ctcctctgac aaagccttga
     1501 ccttcatgaa ggaccatttt ctgatggatg agcacgtggt aggaacaccc ctgctggtga
     1561 agtctggtgt ggagtacaca cggcttgctg tggagtcagc tcggggcctt gatgggagca
     1621 gccatgtggt catgtatctg ggtacctcca cggggtccct gcacaaggct gtggtgcctc
     1681 aggacagcag tgcttatctc gtggaggaga ttcagctgag ccctgactct gagcctgttc
     1741 gaaacctgca gctggccccc gcccagggtg cagtgtttgc aggcttctct ggaggcatct
     1801 ggagagttcc cagggccaat tgcagtgtct acgagagctg tgtggactgt gtgcttgcca
     1861 gggaccctca ctgtgcctgg gaccctgaat caagactctg cagccttctg tctggctcta
     1921 ccaagccttg gaagcaggac atggaacgcg gcaacccgga gtgggtatgc acccgtggcc
     1981 ccatggccag gagcccccgg cgtcagagcc cccctcaact aattaaagaa gtcctgacag
     2041 tccccaactc catcctggag ctgccctgcc cccacctgtc agcactggcc tcttaccact
     2101 ggagtcatgg ccgagccaaa atctcagaag cctctgctac cgtctacaat ggctccctct
     2161 tgctgctgcc gcaggatggt gtcgggggcc tctaccagtg tgtggcgact gagaacggct
     2221 actcataccc tgtggtctcc tattgggtag acagccagga ccagcccctg gcgctggacc
     2281 ctgagctggc gggcgttccc cgtgagcgtg tgcaggtccc gctgaccagg gtcggaggcg
     2341 gagcttccat ggctgcccag cggtcctact ggccccattt tctcatcgtt accgtcctcc
     2401 tggccatcgt gctcctggga gtgctcactc tcctcctcgc ttccccactg ggggcgctgc
     2461 gggctcgggg taaggttcag ggctgtggga tgctgccccc cagggaaaag gctccactga
     2521 gcagggacca gcacctccag ccctccaagg accacaggac ctctgccagt gacgtagatg
     2581 ccgacaacaa ccatctgggc gccgaagtgg cttgacggtc gaccgagatc tctcgaggta
     2641 ccgcggccgc ggggatccag acatgataag atacattgat gagtttggac aaaccacaac
     2701 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2761 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2821 ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgatct gcaggcatgc
     2881 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3421 ctctcctgtt ccgaccctgc cgcttacc
//
