LOCUS       sequence-217025-        7036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an N-terminal GST-tagged full length MePCE protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M, Xhemalce B
  TITLE     Crosstalk between the RNA Methylation and Histone-Binding Activities
            of MePCE Regulates P-TEFb Activation on Chromatin.
  JOURNAL   Cell Rep. 2018 Feb 6;22(6):1374-1383. doi: 
            10.1016/j.celrep.2018.01.028.
  PUBMED    29425494
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-6- 6"; volume: "22"; issue: "6"; pages: "1374-1383"
COMMENT     Sequence Label: pGEX-5X-1-Full-Length-MePCE
FEATURES             Location/Qualifiers
     source          1..7036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(185..190,191..206,207..213)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             260..913
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     871..893
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             923..934
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3088..3110)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3255..3273)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3341..3445
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3446..3514,3515..4306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3664..3683)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4477..5065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4966..4985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5219..5236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5309..5386
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5387..6469
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5406..5425)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6518..6523,6524..6541,6542..6548)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6556..6572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6580..6596
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6580..6596
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6592..7036,1..83)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(6612..6629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6612..6628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6621..6643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttagcttatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
       61 tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
      121 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
      181 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagtattca tgtcccctat actaggttat tggaaaatta agggccttgt
      301 gcaacccact cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga
      361 gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa
      421 tcttccttat tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta
      481 tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat
      541 gcttgaagga gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga
      601 ctttgaaact ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga
      661 agatcgttta tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat
      721 gttgtatgac gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc
      781 aaaattagtt tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa
      841 atccagcaag tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga
      901 ccatcctcca aaatcggatc tgatcgaagg tcgtgggatc tccatcgaga tggcggcgga
      961 gaaggagccg tttctggtgc cggccccgcc gccgccgctc aaagatgagt cgggcggagg
     1021 gggcggcccc acggtgccac cgcaccaaga ggccgcctct ggggagctcc gcggcgggac
     1081 ggagcgtggt ccgggtcgtt gcgcgccatc tgcggggtcc ccagccgctg cggtcggtcg
     1141 ggaaagcccc ggggccgcgg ccacctcctc cagtggtccc caggcgcagc agcaccgagg
     1201 gggcggcccc caggcgcagt cgcatgggga ggcccgcctg tcggatcccc cggggcgagc
     1261 cgctcccccg gacgtggggg aggagcgccg gggagggggc gggacagagc tgggtccccc
     1321 tgctcctcct cgaccccgca atggctatca gccccaccgg ccacctgggg ggggcggggg
     1381 caagaggaga aatagctgta atgtaggggg aggcggggga ggcttcaaac atccggcctt
     1441 caagaggcgc aggcgggtga attcggactg tgactctgtg ttaccttcca acttcctcct
     1501 ggggggcaat atctttgatc ccctgaacct gaatagcctc ctggatgagg aagtgagccg
     1561 cactctcaac gcggagaccc ctaagtcatc cccccttccg gccaaagggc gagatccggt
     1621 ggagatcctc atccccaaag atattactga cccgctcagt ctcaatactt gcactgatga
     1681 gggccatgta gttcttgctt cgccactcaa gactggtcgg aagcggcata gacaccgggg
     1741 acagcaccac cagcagcagc aggcagccgg agggagtgag agccaccccg tgccgcccac
     1801 agcccctctc acccccttac tccacgggga gggcgcctca cagcagccgc ggcacagggg
     1861 ccagaaccgg gatgcccccc aaccctatga actcaacaca gccatcaact gcagggatga
     1921 agtggtgtct ccccttccat ctgctctgca gggtccctca ggctccctat cagcccctcc
     1981 agctgcctca gttatctctg cacccccatc ttcctcctcc cgacatcgca aacgtcgcag
     2041 gacttccagc aagtcggagg caggggctag gggtggaggc cagggttcca aggaaaaggg
     2101 ccgagggagt tggggaggcc gccaccacca ccaccaccca ctgcctgcag caggcttcaa
     2161 aaagcaacag cgcaagttcc agtatgggaa ttattgcaaa tactatgggt accgcaatcc
     2221 ttcctgtgag gatgggcgcc ttcgggtgtt gaagcctgag tggtttcggg gccgggacgt
     2281 cctagatctg ggctgcaatg tgggccatct gaccctgagc attgcctgca agtggggccc
     2341 gtcccgcatg gtgggcctgg atatcgattc ccggctcatc cattctgccc gccaaaacat
     2401 ccgacactac ctttccgagg agctgcgtct cccaccccag actttggaag gggacccggg
     2461 ggcagagggt gaggaaggga ccaccaccgt tcgaaagagg agctgcttcc cagcctcgct
     2521 gactgccagc cggggtccca tcgctgcccc ccaagtgccc ttggatggag cggacacatc
     2581 agtcttcccc aacaatgttg tcttcgtcac gggtaattat gtgctggatc gagatgacct
     2641 ggtggaggcc caaacacctg agtatgatgt ggtgctctgc ctcagcctca ccaagtgggt
     2701 gcatctgaac tggggagacg agggcctgaa gcgcatgttt cgccggatct accggcacct
     2761 acgccctggg ggcatcctgg tcctagagcc ccaaccctgg tcgtcgtatg gcaagagaaa
     2821 gactcttaca gaaacgatct acaagaacta ctaccgaatc caattgaagc cagagcagtt
     2881 cagttcctac ctgacatccc cagacgtggg cttctccagc tatgagcttg tggccacacc
     2941 ccacaacacc tctaaaggct tccagcgtcc tgtgtacctg ttccacaagg cccgatcccc
     3001 cagccacgaa ttcccgggtc gactcgagcg gccgcatcgt gactgactga cgatctgcct
     3061 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3121 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3181 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc
     3241 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     3301 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3421 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3481 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3541 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3601 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3661 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     3721 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3781 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3841 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3901 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3961 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     4021 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4081 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4141 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4201 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4261 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4621 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5161 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5221 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     5281 gtgcggtatt tcacaccgca taaattccga caccatcgaa tggcgcaaaa cctttcgcgg
     5341 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
     5401 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     5461 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     5521 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     5581 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     5641 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     5701 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     5761 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     5821 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     5881 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     5941 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     6001 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     6061 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     6121 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     6181 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     6241 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     6301 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     6361 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6421 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6481 ttaatgtaag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6541 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6601 attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6661 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6721 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg
     6781 tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat
     6841 actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac
     6901 gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt
     6961 tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt
     7021 tttgatggcg ttggaa
//
