LOCUS       sequence-217027-       10315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 7560 
            bases in length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-7560.1
FEATURES             Location/Qualifiers
     source          1..10315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7949..7965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7949..7965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7962..7984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7973..7989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7997..8003,8004..8021,8022..8027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8042..8063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8180..8197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8431..8450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9110..9901,9902..9970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9971..10075)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10143..10161
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10199..10221)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc gcatgcctcg agttagacct gattcttgag aaacaaggcg gctccacact
      481 gaatccattg atcctgatta ccaccgcaag gcacatcgat gttggtcacg actctgtcgc
      541 gctcagcgga tgtgtacact ggcaaagaaa tgcactcatc gggtgagtat gggccgcaag
      601 cgtcttgagg aatccaaccc atgaagcatg gcagaacgct agagacgctt gggctgtcga
      661 gctgattctc ggacagttga ttaccgtcga aggagcagcc ttccaggagc agtcctgaga
      721 tcttgatctg gagtttggcc tcttgcagtc tacccttcca ggaggcgaca aatttcagag
      781 aatccactga tcttccaaca gcccttgctg tttcctggcg gagggcgttc aggaatgtgt
      841 cgggatggaa caattcagag aggtccagtg tttcggacaa gagggcctgc ttctctgctt
      901 tatcgaccca attctggatg gccagtgctc tagccacgag gcctctgaga tattgcagag
      961 ggtcttcggg tccctcccac ttggattgcc aggccagagg gcacttctgg ttgagcaggg
     1021 cagaggccaa cttctggact tctgatgaga gcagtgtagt gccgcggatc actttgctca
     1081 aggctgccag tgattgatga actgactgga ccaatctgat ggcgttgaac tgctccaaga
     1141 taataaagga cagaattggt gatccttggc ggtcattggg aggagggacc ttctggtgga
     1201 tcaggttgga gttctggttc agtttcttcc acaggttgag cactgggctg agttcattgc
     1261 tccagatctc gcgatcgaac tttgagcctg cagtaataga gcggcccagg atgcggagtt
     1321 gggaaatcac ctgagatgag atcatgcgct gtgatgagcg tgcaatgttg gcaggcagac
     1381 cgaagaagga tggcttatcg tcttctggaa tcttctcaat gacagccctg taatccagaa
     1441 tgctgcagct ttgtggcagg ctcacgctgt agggaaagat ggacttcttg ttgcgctgat
     1501 tgaacacgtc aatgacgcta gagttgaaga attgcttcaa atagctctgc aggacgcgca
     1561 agtcgaaata gttatcgatg cggcctccgt agatggcgtt ctccagcaga ccatggacaa
     1621 actcccattg cacgtccttg gcaccatcaa agagcctgtc aatgatattg taaccggccc
     1681 tcaggtcgga gagagagaac tcgtagaatt tggtccaacc ttgaggaatg tagttgcgcc
     1741 tctcttggca tgcggcgtga aaccaggcca gagagaacag agcatgtgcg cggtgagtgt
     1801 tatctttctt agagatttgt tcaggtgtcc aagattcata tgtgcgcatg agattcttct
     1861 tgagtccagg tggtgattca taagtgatct tgaggcttga ctggaggagg ataggtgtaa
     1921 agtttgggtg cacttcggct gtcaaccaga gcctgaaagt gtctttgggt tgcaaggtgt
     1981 tcagttcctt ctccaagaca ggcagccagg agacaaccag gtgcaagttc ttcaaacaca
     2041 gccagtcacc attccttgcg cattccttga gcatttggat agccaagtca gcttggccct
     2101 ggcccattgc aacctggtgg taacattctc ctgatctttc ggcgttggcg agctcctgga
     2161 gttcctggga gggatcggct cctggagaaa taatgatcag aatgggctcg atttcgagtg
     2221 tctccttgta cagcctcttg agattgagtg ggagtggaga gacttccttc aaacccaagg
     2281 tcttgcaggc aaagagggcc atagctgact gcaatctatc tggcctgagc acctgcacga
     2341 cgaggatctg ctggaacaag ctgaccttct tagccaagat gctaggaaat tcctgctcac
     2401 acatgctgtt gttgtagtag gtgcgccaca aagcggcgtc ctcgaaacac agagtctggt
     2461 acagggaagg cagagcgatc ttcaaggttg ccacagccca ggatctctct tgatcgatcc
     2521 atgaaggcaa ctggtccctg atcttctgct gggagtcggc tttgcgcaac atgtctccga
     2581 ccaccacgcc agtgaaggta tcccattcgt tctcctggaa caattctgga tgcatacctc
     2641 tgacgaaatg cagggcgaac atcagctggt cggccttgaa caggcagcgg cagatgtatt
     2701 cgtacaccat gtgctgcagg gagctgatca gggattgaat cctctgttct gtgttctcag
     2761 aatcctgctt attctggagt gccctttgaa acaacctcag aaaggcggcc agggagaacc
     2821 tgtacatatt attgatcttg gagaggtcag agataatgaa gtacatcttt gaagcagact
     2881 cagcgagtgg cagatatgcg tcgcgctcct ggtcgagaga gatttgcagc ttataagatt
     2941 ccttcagaga ctcttggatg agggcggaag atgctttagt ctgattcaga gactcgatca
     3001 ggtccttgtt ctccaggatg ttgccctgag aagtagccaa tgtctccagc agagattctt
     3061 cgagtttggc cagttggatc ttcttatcct cctcctgttg gagcagctta gtcttctgtt
     3121 cttccaaatc gggtttctca tgttggatgg tgagggccag gagctggcct ctcagtcctg
     3181 agcgggtggt tgtgaaatta acctctgtga cgatgctggc agcgtctggt ggaatgaatg
     3241 ggttgggatt cctagtgctc aggaacaggc gaaactcctc gttatagtcg atgatcttat
     3301 ctccaatctg gaccacatag cgtgggcctt gggccaccaa atctctgcgc aacagagggt
     3361 acagaactgg ctccacgcca tccatctcct ggatgatcaa ggtcttgccg aagcggacgg
     3421 cgagttccag ggcagtgatg aagttggagt cttgctggtt gatcacttcc agcctggaat
     3481 ccttcaagtg agtcttcagc cactcagtag cctgtgagct gggatcgatg aggaaagggc
     3541 aaacgcggga ttgcaagata accagtgcgt tctcgattga gaggtcatcg ctgggcagac
     3601 cttcagactt ccagatcagc tgctctgact cggtgcagag aaatcttctc aggtcaaact
     3661 tctccaggcc ggcagacttg gtccactcct ccagacatgt ctttctgaga gactcaggtg
     3721 cagcggacaa gtaggtgata aaggctgcgg ccaactgagc cctctttggg agggttgcca
     3781 gctcctcagt aatctcgacg acctgagcat tccatctctt gtgttcgcgg tccagctgat
     3841 taatcaacac ttcagctgct ttgatagtct cctgagcctt ggacacctca gcttccaact
     3901 tagctgcttc tgaggtcctg ctctgaaatt tctccttcag ttctgacacc ttctgaccga
     3961 cagaattgag gagttcttcc agtttcctct ttctatcctc ggtcttcttc aggttgcttt
     4021 ccaggccggc ttgctcagtc tccagagggt ggatgcgttc gagcacgtga gagtactgga
     4081 tattggcctt aacccaggct gccaagggtg cagctgcagt tgatgctctc ttagcgttct
     4141 tgggatcaaa tgaaccttta ttcttgaaca gcagttcctc cacagattcc ctaatttcct
     4201 tggagatgtt cctagcgtcg aaagtagcaa tgtcctccct gacaccgcgc ttggccaaga
     4261 aagacttcat agacacccaa gaggtgtcaa agatacccat caagcgcagg acgccctcca
     4321 ggatgtcgcg gataacgtct ggtggcattc tcagtgacct gatctctgag agagactctg
     4381 gtttgatatt acccacggcg agcttggcct cgttcaccag aggctgcacc tctttgagtt
     4441 cgtcgtcaat cttgttcttt ctctcctcga tcttgaccac ctcctctgcg atgcggtgct
     4501 tcagcctctc cagctctgtc ttctgctcgg aagcatcctg catagacacg gtaatcattt
     4561 gcagggcagc gtcagcttca tcctgcttag tcttcaggag gactgattgt tcgccagcct
     4621 tgcgattcag ttcatccacc agagctttgg cttcgttcag cttgctgact ccggcctgca
     4681 gatgagattg gcgcttcagc agctccttct tctttgagct ggagatggcg gaatagacgt
     4741 ggaggaaggt catgtactgg ctaggagtag ctccgtaagc tttgcaagac tcgtggatca
     4801 gcaggaagct cttgaggaaa tcaggatcga cggagttctt cttcttctcc tctttgcgct
     4861 tcttgtcgtt gtacttctca ccgcctccag tctcgctaaa caacatctcg ggaatcttct
     4921 tcatagaaga attagaccaa ccctccatcc agaggacctg gcacttctta tggagggcgg
     4981 gattgctctc gcaattgatc atgaaattgg aattagcgct gtccataatc agcacaatgt
     5041 ggagattctg ttgaatgcga taggtgaagt aattgaacac tgggccgaag aagccgtcct
     5101 gtgatgcctg atccttcaat gggaggagca gtggctccag ttcctccaaa gtgtacaagc
     5161 cgggcacctc gccagaggac agcagggagt tgatcatctc caggaaggtt gggtggacga
     5221 attgatagtc ctccaagagg aggacgacct gctgggcctc gataccggcg agttgcaaga
     5281 cgtgtttcag atcgttcttg aattgcttga gttcgtagcc tctgctgatc tttggagaga
     5341 acaggacggc tccatgcata tggctgacca gagaagtaat agtgcgtctt ccaactccag
     5401 agcgtccggc cagcagcagt gatccaccag ggaaagacag gacgcgatca attctggaca
     5461 tatactccag gacctcgtgg aacaacagaa tgtccaggtt ctggttgtcg cggccgtagt
     5521 gaatcaggcc cttcttgatc acgtccttca agtcagttga gttcagtttg ccgagaggct
     5581 tgccatgagg tggcaatggt tggcctggag cagcccttgc accggagttg tgcctggctc
     5641 cccatgtcac atagaaggaa tcggacatat tatccaggat atcagagccc caatcacctt
     5701 gaaacacaga ggtgaggata atatcgaaca gatgcagttc cttagcgcca acaatcttat
     5761 ctctaaagag cctgcgggct tcgtaagcca cgatttcgag gacataatcc aatgggtggt
     5821 ttgatgagcc tccctccagg tcatagcgaa acagtcccag aacccattgt gtcagaatgc
     5881 agggtgtgaa gaagtaatgt gagtaatcat cgacggtgaa cttggcgcgg acttgttcgt
     5941 acacctgaac catagagcca gccaagaggt agatcttgct tgatgagccc cagatgctat
     6001 ggttcttcaa attcttgtgg aggacaggtt cgaggtaggc gccatagatg gtctgcaact
     6061 gttcgcgctc gggataatcg attgagcaca gtctcacaat tgatgtaaac ctggtggtga
     6121 gcttatgcct tcccaaccta ccgccggcag acatggaagc cacaatctgg atgttcgcgg
     6181 ccgccacagg atctgtgatg ggaacagaga agggtccaca gctccctgag agctgcgtcc
     6241 ggttgtcacg cactccttca gcagctgctc cagggtcttc ttcatctcca gggccagatc
     6301 gttcagccag gtttcgacgt tattggacaa gggcacctta ttcttgaatg gcacaacttc
     6361 tccctccaag gacttcattg cagtaatgtg cttagacttc tcatcgaaac agacagagtt
     6421 gataccagca aacagcttct tgaggtgaga ctgaatcacg gatggattgg ttgactggcc
     6481 caagatctcc agcaggtcat cgtctccgat gaagtagaag cgagggaagg cgctgcgctt
     6541 ctcctccagg aactcgttga ggctgcgctg gcatctctgc aattggtcca ggatggtcag
     6601 cagtgagttg cgaatgcctg cgtgggtagt caaggttgtg acgcgattgt ccttcttgat
     6661 atctgtcatg atgctgcgga aatcttcatc caccctgttg aatctggtct gctctttggg
     6721 cagggctccg cgaccaaaga ttggttcgag gtaaacccac tttctctgaa tatggttgag
     6781 gttctgcaaa tattcgtcca gttcggcgag tttcctttcc cagatgctga ccttgtcttc
     6841 aaagccttta tagtatggtg agtccttcaa ggactgcagg aggcatctat tgtcgcccac
     6901 ctggttgacg atgtccttcc aatccttgat cagcttcatg gtgcggctct gggagtcctc
     6961 atagtcgatc agtgtgaaca cagcacccac gccccacaaa tccagttccc tgagtgcttc
     7021 gcggatggtc acttcaccct gggcgcgtga gttcagatct ttcaggtcag cagccttggc
     7081 cacgatggta tcggcaactc tcagcaggtc accgaacagc agtttctcca gagatgtgcc
     7141 gcgaggcaga cccagcaagc ggaacagatc cagccagtga tcgggtgaca ggtgttctcc
     7201 gcggacgtac ttcaggatag ggataacaat cttatatttg tccacctcag attggagctt
     7261 gacagtcatc acagaatgct cctccacttt cctgaggcgg tcgtgccaat tcatcagaaa
     7321 ctcctcaaac aagtaggtct tagtgcgaaa ggtgatccaa tcttcattgg ccatttcttg
     7381 gaatccctgc tggaactctt cgtagaaggc ccaaatctga gcacaggact caatatcctt
     7441 gctgatgctg gaggcgagag agaagttagg ttcttcgagg cggaagtggt gacagtcatc
     7501 gaccagcttc ttgcgtgtca cttccaaatc gtcagaaccg gtaataccag cagcagtaac
     7561 aaattctaac aagaccatgt ggtctctctt ttcgtttgga tccacgcgcc ctgtagcggc
     7621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7801 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7921 ggtttaacac tcagaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8761 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10081 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10141 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaa
//
