LOCUS       sequence-217035-        6721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces an N-terminal GST-tagged full length LARP7 protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Shelton SB, Shah NM, Abell NS, Devanathan SK, Mercado M, Xhemalce B
  TITLE     Crosstalk between the RNA Methylation and Histone-Binding Activities
            of MePCE Regulates P-TEFb Activation on Chromatin.
  JOURNAL   Cell Rep. 2018 Feb 6;22(6):1374-1383. doi: 
            10.1016/j.celrep.2018.01.028.
  PUBMED    29425494
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.01"; journalName: "Cell Rep"; date: 
            "2018-02-6- 6"; volume: "22"; issue: "6"; pages: "1374-1383"
COMMENT     Sequence Label: pGEX-5X-1-LARP7
FEATURES             Location/Qualifiers
     source          1..6721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(60..65,66..81,82..88)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             135..788
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     746..768
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             798..809
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2648..2670)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2815..2833)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2901..3005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3006..3074,3075..3866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3224..3243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4037..4625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4526..4545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4779..4796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4869..4946
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4947..6029
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4966..4985)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6078..6083,6084..6101,6102..6108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6116..6132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6140..6156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6140..6156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6152..6679
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(6172..6189)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6172..6188)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6181..6203)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga aatgagctgt
       61 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac
      121 aggaaacagt attcatgtcc cctatactag gttattggaa aattaagggc cttgtgcaac
      181 ccactcgact tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg
      241 atgaaggtga taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc
      301 cttattatat tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag
      361 ctgacaagca caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg
      421 aaggagcggt tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg
      481 aaactctcaa agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc
      541 gtttatgtca taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt
      601 atgacgctct tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat
      661 tagtttgttt taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca
      721 gcaagtatat agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc
      781 ctccaaaatc ggatctgatc gaaggtcgtg ggatccccat ggaaactgaa agtggaaatc
      841 aggaaaaggt aatggaagaa gaaagcactg aaaagaaaaa agaagttgaa aaaaagaaac
      901 ggtcacgagt taaacaggtg cttgcagata ttgctaagca agtggacttc tggtttgggg
      961 atgcaaatct tcacaaggat agatttcttc gagaacagat agaaaaatct agagatggat
     1021 atgttgatat atcactactt gtgtctttta acaaaatgaa aaaattgact actgatggga
     1081 agttaattgc cagagcgttg agaagttcag ctgttgtaga gcttgatttg gaaggcacca
     1141 gaatccggag gaaaaaacct ctgggggaaa gaccaaagga tgaggatgaa cgcacagtgt
     1201 atgtggagtt acttcccaaa aatgttaatc acagctggat tgaaagagta tttgggaaat
     1261 gtggcaatgt tgtttatata agtataccac attataagtc tactggagat ccaaagggat
     1321 ttgcgtttgt ggaatttgaa acaaaagaac aagcagcaaa agcaattgag tttcttaaca
     1381 acccaccaga agaagcacca agaaaacctg gcatatttcc taaaacagtg aaaaataagc
     1441 ccattccagc cttaagagtt gtggaagaga agaaaaagaa aaagaagaag aaaggccgaa
     1501 tgaaaaagga agacaatatc caagccaaag aagaaaacat ggacacaagc aacaccagca
     1561 tcagtaaaat gaaaagatcc agacccacat ctgagggctc tgacattgag tccactgaac
     1621 cccaaaagca gtgctcaaag aaaaagaaaa aacgggacag agttgaagca tctagcttac
     1681 ctgaagtcag aacagggaag aggaagagaa gcagctctga agatgcagaa tccctagctc
     1741 cccgatcaaa agtaaagaaa attattcaga aagacatcat taaggaagca tcagaagctt
     1801 ccaaggaaaa tagagatata gaaatctcta ctgaagagga aaaggatact ggagatctaa
     1861 aagatagctc tctcttgaaa acaaaaagga aacataagaa aaaacataaa gagagacata
     1921 aaatgggaga agaagttata ccattaagag tgctatcaaa gagcgaatgg atggatttga
     1981 aaaaagagta tttagcgcta caaaaagcta gcatggcttc tttaaaaaaa acaatatccc
     2041 aaataaaatc agagtcagaa atggaaacag acagtggagt acctcaaaac actggaatga
     2101 aaaatgaaaa aacagccaac agggaagagt gtcgcaccca ggagaaagtt aatgcaacag
     2161 gaccacagtt cgtgagtgga gtgattgtga agatcattag cacagagcct ctacctggca
     2221 ggaaacaagt ccgggatact ttggcagcaa tctcagaagt tctttatgtt gatttgctag
     2281 aaggggatac agaatgccat gctagattta aaactcctga ggatgctcaa gcagtaataa
     2341 atgcctatac agaaattaac aagaaacact gctggaaact cgagatcctt tctggtgatc
     2401 acgaacaaag gtattggcag aagattttgg ttgatagaca ggcaaaactt aatcagcctc
     2461 gggaaaagaa aagaggcact gaaaagttaa tcaccaaagc tgaaaagatt aggctggcaa
     2521 agactcaaca agcgagtaaa catataagat tttctgaata tgattgagaa ttcccgggtc
     2581 gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
     2641 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2701 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     2761 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
     2821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3241 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3541 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4801 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4861 taaattccga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg
     4921 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
     4981 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
     5041 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
     5101 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
     5161 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
     5221 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
     5281 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
     5341 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
     5401 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
     5461 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
     5521 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
     5581 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
     5641 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
     5701 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
     5761 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
     5821 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
     5881 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     5941 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6001 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact
     6061 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6121 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt
     6181 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6241 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6301 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
     6361 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
     6421 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt
     6481 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
     6541 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaattag
     6601 cttatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt
     6661 atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
     6721 t
//
