LOCUS       sequence-21704-e         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Mayhew MW, Webb DJ, Kovalenko M, Whitmore L, Fox JW, Horwitz AF
  TITLE     Identification of protein networks associated with the 
            PAK1-betaPIX-GIT1-paxillin signaling complex by mass spectrometry.
  JOURNAL   J Proteome Res. 2006 Sep . 5(9):2417-23.
  PUBMED    16944954
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res. 2006 Sep . 5(9):2417-23."
COMMENT     Sequence Label: Flag-betaPIXa
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg ccaccatgga ctacaaggac gacgatgaca agggacctaa
      121 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgcatgac
      181 tgataacacc aacagccaac tggtagtacg agccaagttt aacttccagc agaccaatga
      241 agatgaactc tccttctcaa agggtgatgt catccatgtc acacgagtgg aggaaggagg
      301 ctggtgggag ggcacacaca atggcaggac cggctggttc cccagcaact acgttcgaga
      361 gatcaagcca agtgagaagc ccgtgtcacc caaatcaggg accttgaaga gccctcccaa
      421 agggttcgat acgactgcca tcaacaagag ctattacaac gtggtgctac agaacatcct
      481 ggaaacagag catgagtatt ccaaggagct gcagtctgtg ctgtccacct acctgcggcc
      541 actgcagacc agtgacaagt tgagttcagc aaacacttca tatttaatgg gaaatctaga
      601 ggaaatatct tccttccagc aagtgcttgt acagtcccta gaagaatgca cgaagtctcc
      661 tgaagcccaa cagagagttg gtggctgctt cctgagcctg atgccgcaga tgaggaccct
      721 gtacctcgct tactgtgcca accacccatc tgctgtgagc g
//
