LOCUS       sequence-217042-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 8064 
            bases in length (v4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-8064.4
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8453..8469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8453..8469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8466..8488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8477..8493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8501..8507,8508..8525,8526..8531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8546..8567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8684..8701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8935..8954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9614..10405,10406..10474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10475..10579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10647..10665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10703..10725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc gcatgcctcg agttagacct gattcttgag aaacaaggcg gctccacact
      481 gaatccattg atcctgatta ccaccgcaag gcacatcgat gttggtcacg actctgtcgc
      541 gctcagcgga tgtgtacact ggcaaagaaa tgcactcatc gggtgagtat gggccgcaag
      601 cgtcttgagg aatccaaccc atgaagcatg gcagaacgct agagacgctt gggctgtcga
      661 gctgattctc ggacagttga ttaccgtcga aggagcagcc ttccaggagc agtcctgaga
      721 tcttgatctg gagtttggcc tcttgcagtc tacccttcca ggaggcgaca aatttcagag
      781 aatccactga tcttccaaca gcccttgctg tttcctggcg gagggcgttc aggaatgtgt
      841 cgggatggaa caattcagag aggtccagtg tttcggacaa gagggcctgc ttctctgctt
      901 tatcgaccca attctggatg gccagtgctc tagccacgag gcctctgaga tattgcagag
      961 ggtcttcggg tccctcccac ttggattgcc aggccagagg gcacttctgg ttgagcaggg
     1021 cagaggccaa cttctggact tctgatgaga gcagtgtagt gccgcggatc actttgctca
     1081 aggctgccag tgattgatga actgactgga ccaatctgat ggcgttgaac tgctccaaga
     1141 taataaagga cagaattggt gatccttggc ggtcattggg aggagggacc ttctggtgga
     1201 tcaggttgga gttctggttc agtttcttcc acaggttgag cactgggctg agttcattgc
     1261 tccagatctc gcgatcgaac tttgagcctg cagtaataga gcggcccagg atgcggagtt
     1321 gggaaatcac ctgagatgag atcatgcgct gtgatgagcg tgcaatgttg gcaggcagac
     1381 cgaagaagga tggcttatcg tcttctggaa tcttctcaat gacagccctg taatccagaa
     1441 tgctgcagct ttgtggcagg ctcacgctgt agggaaagat ggacttcttg ttgcgctgat
     1501 tgaacacgtc aatgacgcta gagttgaaga attgcttcaa atagctctgc aggacgcgca
     1561 agtcgaaata gttatcgatg cggcctccgt agatggcgtt ctccagcaga ccatggacaa
     1621 actcccattg cacgtccttg gcaccatcaa agagcctgtc aatgatattg taaccggccc
     1681 tcaggtcgga gagagagaac tcgtagaatt tggtccaacc ttgaggaatg tagttgcgcc
     1741 tctcttggca tgcggcgtga aaccaggcca gagagaacag agcatgtgcg cggtgagtgt
     1801 tatctttctt agagatttgt tcaggtgtcc aagattcata tgtgcgcatg agattcttct
     1861 tgagtccagg tggtgattca taagtgatct tgaggcttga ctggaggagg ataggtgtaa
     1921 agtttgggtg cacttcggct gtcaaccaga gcctgaaagt gtctttgggt tgcaaggtgt
     1981 tcagttcctt ctccaagaca ggcagccagg agacaaccag gtgcaagttc ttcaaacaca
     2041 gccagtcacc attccttgcg cattccttga gcatttggat agccaagtca gcttggccct
     2101 ggcccattgc aacctggtgg taacattctc ctgatctttc ggcgttggcg agctcctgga
     2161 gttcctggga gggatcggct cctggagaaa taatgatcag aatgggctcg atttcgagtg
     2221 tctccttgta cagcctcttg agattgagtg ggagtggaga gacttccttc aaacccaagg
     2281 tcttgcaggc aaagagggcc atagctgact gcaatctatc tggcctgagc acctgcacga
     2341 cgaggatctg ctggaacaag ctgaccttct tagccaagat gctaggaaat tcctgctcac
     2401 acatgctgtt gttgtagtag gtgcgccaca aagcggcgtc ctcgaaacac agagtctggt
     2461 acagggaagg cagagcgatc ttcaaggttg ccacagccca ggatctctct tgatcgatcc
     2521 atgaaggcaa ctggtccctg atcttctgct gggagtcggc tttgcgcaac atgtctccga
     2581 ccaccacgcc agtgaaggta tcccattcgt tctcctggaa caattctgga tgcatacctc
     2641 tgacgaaatg cagggcgaac atcagctggt cggccttgaa caggcagcgg cagatgtatt
     2701 cgtacaccat gtgctgcagg gagctgatca gggattgaat cctctgttct gtgttctcag
     2761 aatcctgctt attctggagt gccctttgaa acaacctcag aaaggcggcc agggagaacc
     2821 tgtacatatt attgatcttg gagaggtcag agataatgaa gtacatcttt gaagcagact
     2881 cagcgagtgg cagatatgcg tcgcgctcct ggtcgagaga gatttgcagc ttataagatt
     2941 ccttcagaga ctcttggatg agggcggaag atgctttagt ctgattcaga gactcgatca
     3001 ggtccttgtt ctccaggatg ttgccctgag aagtagccaa tgtctccagc agagattctt
     3061 cgagtttggc cagttggatc ttcttatcct cctcctgttg gagcagctta gtcttctgtt
     3121 cttccaaatc gggtttctca tgttggatgg tgagggccag gagctggcct ctcagtcctg
     3181 agcgggtggt tgtgaaatta acctctgtga cgatgctggc agcgtctggt ggaatgaatg
     3241 ggttgggatt cctagtgctc aggaacaggc gaaactcctc gttatagtcg atgatcttat
     3301 ctccaatctg gaccacatag cgtgggcctt gggccaccaa atctctgcgc aacagagggt
     3361 acagaactgg ctccacgcca tccatctcct ggatgatcaa ggtcttgccg aagcggacgg
     3421 cgagttccag ggcagtgatg aagttggagt cttgctggtt gatcacttcc agcctggaat
     3481 ccttcaagtg agtcttcagc cactcagtag cctgtgagct gggatcgatg aggaaagggc
     3541 aaacgcggga ttgcaagata accagtgcgt tctcgattga gaggtcatcg ctgggcagac
     3601 cttcagactt ccagatcagc tgctctgact cggtgcagag aaatcttctc aggtcaaact
     3661 tctccaggcc ggcagacttg gtccactcct ccagacatgt ctttctgaga gactcaggtg
     3721 cagcggacaa gtaggtgata aaggctgcgg ccaactgagc cctctttggg agggttgcca
     3781 gctcctcagt aatctcgacg acctgagcat tccatctctt gtgttcgcgg tccagctgat
     3841 taatcaacac ttcagctgct ttgatagtct cctgagcctt ggacacctca gcttccaact
     3901 tagctgcttc tgaggtcctg ctctgaaatt tctccttcag ttctgacacc ttctgaccga
     3961 cagaattgag gagttcttcc agtttcctct ttctatcctc ggtcttcttc aggttgcttt
     4021 ccaggccggc ttgctcagtc tccagagggt ggatgcgttc gagcacgtga gagtactgga
     4081 tattggcctt aacccaggct gccaagggtg cagctgcagt tgatgctctc ttagcgttct
     4141 tgggatcaaa tgaaccttta ttcttgaaca gcagttcctc cacagattcc ctaatttcct
     4201 tggagatgtt cctagcgtcg aaagtagcaa tgtcctccct gacaccgcgc ttggccaaga
     4261 aagacttcat agacacccaa gaggtgtcaa agatacccat caagcgcagg acgccctcca
     4321 ggatgtcgcg gataacgtct ggtggcattc tcagtgacct gatctctgag agagactctg
     4381 gtttgatatt acccacggcg agcttggcct cgttcaccag aggctgcacc tctttgagtt
     4441 cgtcgtcaat cttgttcttt ctctcctcga tcttgaccac ctcctctgcg atgcggtgct
     4501 tcagcctctc cagctctgtc ttctgctcgg aagcatcctg catagacacg gtaatcattt
     4561 gcagggcagc gtcagcttca tcctgcttag tcttcaggag gactgattgt tcgccagcct
     4621 tgcgattcag ttcatccacc agagctttgg cttcgttcag cttgctgact ccggcctgca
     4681 gatgagattg gcgcttcagc agctccttct tctttgagct ggagatggcg gaatagacgt
     4741 ggaggaaggt catgtactgg ctaggagtag ctccgtaagc tttgcaagac tcgtggatca
     4801 gcaggaagct cttgaggaaa tcaggatcga cggagttctt cttcttctcc tctttgcgct
     4861 tcttgtcgtt gtacttctca ccgcctccag tctcgctaaa caacatctcg ggaatcttct
     4921 tcatagaaga attagaccaa ccctccatcc agaggacctg gcacttctta tggagggcgg
     4981 gattgctctc gcaattgatc atgaaattgg aattagcgct gtccataatc agcacaatgt
     5041 ggagattctg ttgaatgcga taggtgaagt aattgaacac tgggccgaag aagccgtcct
     5101 gtgatgcctg atccttcaat gggaggagca gtggctccag ttcctccaaa gtgtacaagc
     5161 cgggcacctc gccagaggac agcagggagt tgatcatctc caggaaggtt gggtggacga
     5221 attgatagtc ctccaagagg aggacgacct gctgggcctc gataccggcg agttgcaaga
     5281 cgtgtttcag atcgttcttg aattgcttga gttcgtagcc tctgctgatc tttggagaga
     5341 acaggacggc tccatgcata tggctgacca gagaagtaat agtgcgtctt ccaactccag
     5401 agcgtccggc cagcagcagt gatccaccag ggaaagacag gacgcgatca attctggaca
     5461 tatactccag gacctcgtgg aacaacagaa tgtccaggtt ctggttgtcg cggccgtagt
     5521 gaatcaggcc cttcttgatc acgtccttca agtcagttga gttcagtttg ccgagaggct
     5581 tgccatgagg tggcaatggt tggcctggag cagcccttgc accggagttg tgcctggctc
     5641 cccatgtcac atagaaggaa tcggacatat tatccaggat atcagagccc caatcacctt
     5701 gaaacacaga ggtgaggata atatcgaaca gatgcagttc cttagcgcca acaatcttat
     5761 ctctaaagag cctgcgggct tcgtaagcca cgatttcgag gacataatcc aatgggtggt
     5821 ttgatgagcc tccctccagg tcatagcgaa acagtcccag aacccattgt gtcagaatgc
     5881 agggtgtgaa gaagtaatgt gagtaatcat cgacggtgaa cttggcgcgg acttgttcgt
     5941 acacctgaac catagagcca gccaagaggt agatcttgct tgatgagccc cagatgctat
     6001 ggttcttcaa attcttgtgg aggacaggtt cgaggtaggc gccatagatg gtctgcaact
     6061 gttcgcgctc gggataatcg attgagcaca gtctcacaat tgatgtaaac ctggtggtga
     6121 gcttatgcct tcccaaccta ccgccggcag acatggaagc cacaatctgg atgttcgcgg
     6181 ccgcctgccc agcagacctc cgagtgcctt aacgctctct gtcttaccag tgccagctgg
     6241 accgtatggg ttgcctccca ggcccatctt catagcctgt gtcaaggtga ggtaacactt
     6301 gtcagtgagt ggagtgtaaa cgagcttgct ggcatttcct tggtactcat aggtgtactg
     6361 gaactcgctg tcaaccatct gaacgcaaca ggtgtggtcg gacttcatgt agaacctcaa
     6421 ctgcttcttc caggcccaat cttcggtggt gtgcacttgg atttggttca gctgcttgac
     6481 cacgtcgatg ttatggataa tgtcgagaat cagtgctttc agtttcagct ccagaatacc
     6541 ggactctgtg ttacctgggt cctcggaaga tgtgtcgatg ttagtatact gctccaattt
     6601 gttcaccagc tgtgtctcaa tctggtgcag ggagtgatct ttaattgcgt tctccacatc
     6661 ttcggtaaac ttgatctgtt cggccaagca caggatctgt gatgggaaca gagaagggtc
     6721 cacagctccc tgagagctgc gtccggttgt cacgcactcc ttcagcagct gctccagggt
     6781 cttcttcatc tccagggcca gatcgttcag ccaggtttcg acgttattgg acaagggcac
     6841 cttattcttg aatggcacaa cttctccctc caaggacttc attgcagtaa tgtgcttaga
     6901 cttctcatcg aaacagacag agttgatacc agcaaacagc ttcttgaggt gagactgaat
     6961 cacggatgga ttggttgact ggcccaagat ctccagcagg tcatcgtctc cgatgaagta
     7021 gaagcgaggg aaggcgctgc gcttctcctc caggaactcg ttgaggctgc gctggcatct
     7081 ctgcaattgg tccaggatgg tcagcagtga gttgcgaatg cctgcgtggg tagtcaaggt
     7141 tgtgacgcga ttgtccttct tgatatctgt catgatgctg cggaaatctt catccaccct
     7201 gttgaatctg gtctgctctt tgggcagggc tccgcgacca aagattggtt cgaggtaaac
     7261 ccactttctc tgaatatggt tgaggttctg caaatattcg tccagttcgg cgagtttcct
     7321 ttcccagatg ctgaccttgt cttcaaagcc tttatagtat ggtgagtcct tcaaggactg
     7381 caggaggcat ctattgtcgc ccacctggtt gacgatgtcc ttccaatcct tgatcagctt
     7441 catggtgcgg ctctgggagt cctcatagtc gatcagtgtg aacacagcac ccacgcccca
     7501 caaatccagt tccctgagtg cttcgcggat ggtcacttca ccctgggcgc gtgagttcag
     7561 atctttcagg tcagcagcct tggccacgat ggtatcggca actctcagca ggtcaccgaa
     7621 cagcagtttc tccagagatg tgccgcgagg cagacccagc aagcggaaca gatccagcca
     7681 gtgatcgggt gacaggtgtt ctccgcggac gtacttcagg atagggataa caatcttata
     7741 tttgtccacc tcagattgga gcttgacagt catcacagaa tgctcctcca ctttcctgag
     7801 gcggtcgtgc caattcatca gaaactcctc aaacaagtag gtcttagtgc gaaaggtgat
     7861 ccaatcttca ttggccattt cttggaatcc ctgctggaac tcttcgtaga aggcccaaat
     7921 ctgagcacag gactcaatat ccttgctgat gctggaggcg agagagaagt taggttcttc
     7981 gaggcggaag tggtgacagt catcgaccag cttcttgcgt gtcacttcca aatcgtcaga
     8041 accggtaata ccagcagcag taacaaattc taacaagacc atgtggtctc tcttttcgtt
     8101 tggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8401 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     8461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10621 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10681 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10741 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10801 ggtgtcgggg ctggcttaa
//
