LOCUS       sequence-217049-        8299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 5544 
            bases in length (v2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-5544.2
FEATURES             Location/Qualifiers
     source          1..8299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5933..5949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5933..5949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5946..5968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5957..5973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5981..5987,5988..6005,6006..6011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6026..6047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6164..6181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6335..6923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6415..6434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7094..7885,7886..7954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7717..7736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7955..8059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8127..8145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8183..8205)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ttttttagat cttccggtga tgagcgatat cccggcactg tcagatttga
      481 tcaccagtat ggtggccagc ggctatgact accggcgcga cgatgatgcg ggcttgtgga
      541 gttcagccga tctgacttat gtcattacct atgaaatgtg aggacgctat gcctgtacca
      601 aatcctacaa tgccggtgaa aggtgccggg accaccctgt gggtttataa ggggagcggt
      661 gacccttacg cgaatccgct ttcagacgtt gactggtcgc gtctggcaaa agttaaagac
      721 ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca gctatctcga tgatgaagat
      781 gcagactgga ctgcgaccgg gcaggggcag aaatctgccg gagataccag cttcacgctg
      841 gcgtggatgc ccggagagca ggggcagcag gcgctgctgg cgtggtttaa tgaaggcgat
      901 acccgtgcct ataaaatccg cttcccgaac ggcacggtcg atgtgttccg tggctgggtc
      961 agcagtatcg gtaaggcggt gacggcgaag gaagtgatca cccgcacggt gaaagtcacc
     1021 aatgtgggac gtccgtcgat ggcagaagat cgcagcacgg taacagcggc aaccggcatg
     1081 accgtgacgc ctgccagcac ctcggtggtg aaagggcaga gcaccacgct gaccgtggcc
     1141 ttccagccgg agggcgtaac cgacaagagc tttcgtgcgg tgtctgcgga taaaacaaaa
     1201 gccaccgtgt cggtcagtgg tatgaccatc accgtgaacg gcgttgctgc aggcaaggtc
     1261 aacattccgg ttgtatccgg taatggtgag tttgctgcgg ttgcagaaat taccgtcacc
     1321 gccagttaat ccggagagtc agcgatgttc ctgaaaaccg aatcatttga acataacggt
     1381 gtgaccgtca cgctttctga actgtcagcc ctgcagcgca ttgagcatct cgccctgatg
     1441 aaacggcagg cagaacaggc ggagtcagac agcaaccgga agtttactgt ggaagacgcc
     1501 atcagaaccg gcgcgtttct ggtggcgatg tccctgtggc ataaccatcc gcagaagacg
     1561 cagatgccgt ccatgaatga agccgttaaa cagattgagc aggaagtgct taccacctgg
     1621 cccacggagg caatttctca tgctgaaaac gtggtgtacc ggctgtctgg tatgtatgag
     1681 tttgtggtga ataatgcccc tgaacagaca gaggacgccg ggcccgcaga gcctgtttct
     1741 gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa actggcgcgt gagatggggc
     1801 gacccgactg gcgtgccatg cttgccggga tgtcatccac ggagtatgcc gactggcacc
     1861 gcttttacag tacccattat tttcatgatg ttctgctgga tatgcacttt tccgggctga
     1921 cgtacaccgt gctcagcctg tttttcagcg atccggatat gcatccgctg gatttcagtc
     1981 tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg
     2041 cagggcttgc cggaggtgtc cgctttggcc cggacgggaa tgaagttatc cccgcttccc
     2101 cggatgtggc ggacatgacg gaggatgacg taatgctgat gacagtatca gaagggatcg
     2161 caggaggagt ccggtatggc tgaaccggta ggcgatctgg tcgttgattt gagtctggat
     2221 gcggccagat ttgacgagca gatggccaga gtcaggcgtc atttttctgg tacggaaagt
     2281 gatgcgaaaa aaacagcggc agtcgttgaa cagtcgctga gccgacaggc gctggctgca
     2341 cagaaagcgg ggatttccgt cgggcagtat aaagccgcca tgcgtatgct gcctgcacag
     2401 ttcaccgacg tggccacgca gcttgcaggc gggcaaagtc cgtggctgat cctgctgcaa
     2461 cagggggggc aggtgaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc
     2521 ggtgcgatca ccctgccgat ggtgggggcc acctcgctgg cggtggcgac cggtgcgctg
     2581 gcgtatgcct ggtatcaggg caactcaacc ctgtccgatt tcaacaaaac gctggtcctt
     2641 tccggcaatc aggcgggact gacggcagat cgtatgctgg tcctgtccag agccgggcag
     2701 gcggcagggc tgacgtttaa ccagaccagc gagtcactca gcgcactggt taaggcgggg
     2761 gtaagcggtg aggctcagat tgcgtccatc agccagagtg tggcgcgttt ctcctctgca
     2821 tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga agctgaccac agacccgacg
     2881 tcggggctga cggcgatggc tcgccagttc cataacgtgt cggcggagca gattgcgtat
     2941 gttgctcagt tgcagcgttc cggcgatgaa gccggggcat tgcaggcggc gaacgaggcc
     3001 gcaacgaaag ggtttgatga ccagacggat ctagtgatga agaatgaaca tcccgcgttc
     3061 ttccctccga acaggacgat attgtaaatt cacttaatta cgagggcatt gcagtaattg
     3121 agttgcagtt ttaccacttt cctgacagtg acagactgcg tgttggctct gtcacagact
     3181 aaatagtttg aatgattagc agttatggtg atcagtcaac caccagggaa taatccttca
     3241 tattattatc gtgcttcacc aacgctgcct caattgctct gaatgcttcc agagacacct
     3301 tatgttctat acatgcaatt acaacatcag ggtaactcat agaaatggtg ctattaagca
     3361 tattttttac acgaatcaga tccacggagg gatcatcagc agattgttct ttattcattt
     3421 tgtcgctcca tgcgcttgct cttcatctag cggttaaaat attacttcaa atctttctgt
     3481 atgaagattt gagcacgttg gccttacata catctgtcgg ttgtatttcc ctccagaatg
     3541 ccagcaggac cgcactttgt tacgcaacca atactattaa gtgaaaacat tcctaatatt
     3601 tgacataaat catcaacaaa acacaaggag gtcagaccag attgaaacga taaaaacgat
     3661 aatgcaaact acgcgccctc gtatcacatg gaaggtttta ccaatggctc aggttgccat
     3721 ttttaaagaa atattcgatc aagtgcgaaa agatttagac tgtgaattgt tttattctga
     3781 actaaaacgt cacaacgtct cacattatat ttactatcta gccacagata atattcacat
     3841 cgtgttagaa aacgataaca ccgtgttaat aaaaggactt aaaaaggttg taaatgttaa
     3901 attctcaaga aacacgcatc ttatagaaac gtcctatgat aggttgaaat caagagaaat
     3961 cacatttcag caatacaggg aaaatcttgc taaagcagga gttttccgat gggttacaaa
     4021 tatccatgaa cataaaagat attactatac ctttgataat tcattactat ttactgagag
     4081 cattcagaac actacacaaa tctttccacg ctaaatcata acgtccggtt tcttccgtgt
     4141 cagcaccggg gcgttggcat aatgcaatac gtgtacgcgc taaaccctgt gtgcatcgtt
     4201 ttaattattc ccggacactc ccgcagagaa gttccccgtc agggctgtgg acatagttaa
     4261 tccgggaata caatgacgat tcatcgcacc tgacatacat taataaatat taacaatatg
     4321 aaatttcaac tcattgttta gggtttgttt aattttctac acatacgatt ctgcgaactt
     4381 caaaaagcat cgggaataac accatgaaaa aaatgctact cgctactgcg ctggccctgc
     4441 ttattacagg atgtgctcaa cagacgttta ctgttcaaaa caaaccggca gcagtagcac
     4501 caaaggaaac catcacccat catttcttcg tttctggaat tgggcagaag aaaactgtcg
     4561 atgcagccaa aatttgtggc ggcgcagaaa atgttgttaa aacagaaacc cagcaaacat
     4621 tcgtaaatgg attgctcggt tttattactt taggcattta tactccgctg gaagcgcgtg
     4681 tgtattgctc acaataattg catgagttgc ccatcgatat gggcaactct atctgcactg
     4741 ctcattaata tacttctggg ttccttccag ttgtttttgc atagtgatca gcctctctct
     4801 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg ggggaggctg cattatccac
     4861 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4921 acgaccagcg gcaacatcat cacgcagagc atcattttca gctttagcat cagctaactc
     4981 cttcgtgtat tttgcatcga gcgcagcaac atcacgctga cgcatctgca tgtcagtaat
     5041 tgccgcgttc gccagcttca gttctctggc atttttgtcg cgctgggctt tgtaggtaat
     5101 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     5161 agcggagata atcgcggtga ctctgctcat acatcaatct ctctgaccgt tccgcccgct
     5221 tctttgaatt ttgcaatcag gctgtcagcc ttatgctcga actgaccata accagcgccc
     5281 ggcagtgaag cccagatatt gctgcaacgg tcgattgcct gacggatatc accacgatca
     5341 atcataggta aagcgccacg ctccttaatc tgctgcaatg ccacagcgtc ctgacttttc
     5401 ggagagaagt ctttcaggcc aagctgcttg cggtaggcat cccaccaacg ggaaagaagc
     5461 tggtagcgtc cggcgcctgt tgatttgagt tttgggttta gcgtgacaag tttgcgaggg
     5521 tgatcggagt aatcagtaaa tagctctccg cctacaatga cgtcataacc atgatttctg
     5581 gggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     5941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7981 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8041 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8101 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     8161 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8221 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8281 ggtgtcgggg ctggcttaa
//
