LOCUS       sequence-217059-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 8064 
            bases in length (v1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-8064.1
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8453..8469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8453..8469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8466..8488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8477..8493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8501..8507,8508..8525,8526..8531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8546..8567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8684..8701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8935..8954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9614..10405,10406..10474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10475..10579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10647..10665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10703..10725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ttttttagat ctaatgcggc atacctcagt ggcgtggagt gcaggtatac
      481 agattaatcc ggcagcgtcc gtcgttgttg atattgctta tgaaggctcc ggcagtggcg
      541 actggcgtac tgacggattc atcgttgggg tcggttataa attctgatta gccaggtaac
      601 acagtgttat gacagcccgc cggaaccggt gggctttttt gtggggtgaa tatggcagta
      661 aagatttcag gagtcctgaa agacggcaca ggaaaaccgg tacagaactg caccattcag
      721 ctgaaagcca gacgtaacag caccacggtg gtggtgaaca cggtgggctc agagaatccg
      781 gatgaagccg ggcgttacag catggatgtg gagtacggtc agtacagtgt catcctgcag
      841 gttgacggtt ttccaccatc gcacgccggg accatcaccg tgtatgaaga ttcacaaccg
      901 gggacgctga atgattttct ctgtgccatg acggaggatg atgcccggcc ggaggtgctg
      961 cgtcgtcttg aactgatggt ggaagaggtg gcgcgtaacg cgtccgtggt ggcacagagt
     1021 acggcagacg cgaagaaatc agccggcgat gccagtgcat cagctgctca ggtcgcggcc
     1081 cttgtgactg atgcaactga ctcagcacgc gccgccagca cgtccgccgg acaggctgca
     1141 tcgtcagctc aggaagcgtc ctccggcgca gaagcggcat cagcaaaggc cactgaagcg
     1201 gaaaaaagtg ccgcagccgc agagtcctca aaaaacgcgg cggccaccag tgccggtgcg
     1261 gcgaaaacgt cagaaacgaa tgctgcagcg tcacaacaat cagccgccac gtctgcctcc
     1321 accgcggcca cgaaagcgtc agaggccgcc acttcagcac gagatgcggt ggcctcaaaa
     1381 gaggcagcaa aatcatcaga aacgaacgca tcatcaagtg ccggtcgtgc agcttcctcg
     1441 gcaacggcgg cagaaaattc tgccagggcg gcaaaaacgt ccgagacgaa tgccaggtca
     1501 tctgaaacag cagcggaacg gagcgcctct gccgcggcag acgcaaaaac agcggcggcg
     1561 gggagtgcgt caacggcatc cacgaaggcg acagaggctg cgggaagtgc ggtatcagca
     1621 tcgcagagca aaagtgcggc agaagcggcg gcaatacgtg caaaaaattc ggcaaaacgt
     1681 gcagaagata tagcttcagc tgtcgcgctt gaggatgcgg acacaacgag aaaggggata
     1741 gtgcagctca gcagtgcaac caacagcacg tctgaaacgc ttgctgcaac gccaaaggcg
     1801 gttaaggtgg taatggatga aacgaacaga aaagcccact ggacagtccg gcactgaccg
     1861 gaacgccaac agcaccaacc gcgctcaggg gaacaaacaa tacccagatt gcgaacaccg
     1921 cttttgtact ggccgcgatt gcagatgtta tcgacgcgtc acctgacgca ctgaatacgc
     1981 tgaatgaact ggccgcagcg ctcgggaatg atccagattt tgctaccacc atgactaacg
     2041 cgcttgcggg taaacaaccg aagaatgcga cactgacggc gctggcaggg ctttccacgg
     2101 cgaaaaataa attaccgtat tttgcggaaa atgatgccgc cagcctgact gaactgactc
     2161 aggttggcag ggatattctg gcaaaaaatt ccgttgcaga tgttcttgaa taccttgggg
     2221 ccggtgagaa ttcggccttt ccggcaggtg cgccgatccc gtggccatca gatatcgttc
     2281 cgtctggcta cgtcctgatg caggggcagg cgtttgacaa atcagcctac ccaaaacttg
     2341 ctgtcgcgta tccatcgggt gtgcttcctg atatgcgagg ctggacaatc aaggggaaac
     2401 ccgccagcgg tcgtgctgta ttgtctcagg aacaggatgg aattaagtcg cacacccaca
     2461 gtgccagtgc atccggtacg gatttgggga cgaaaaccac atcgtcgttt gattacggga
     2521 cgaaaacaac aggcagtttc gattacggca ccaaatcgac gaataacacg ggggctcatg
     2581 ctcacagtct gagcggttca acaggggccg cgggtgctca tgcccacaca agtggtttaa
     2641 ggatgaacag ttctggctgg agtcagtatg gaacagcaac cattacagga agtttatcca
     2701 cagttaaagg aaccagcaca cagggtattg cttatttatc gaaaacggac agtcagggca
     2761 gccacagtca ctcattgtcc ggtacagccg tgagtgccgg tgcacatgcg catacagttg
     2821 gtattggtgc gcaccagcat ccggttgtta tcggtgctca tgcccattct ttcagtattg
     2881 gttcacacgg acacaccatc accgttaacg ctgcgggtaa cgcggaaaac accgtcaaaa
     2941 acattgcatt taactatatt gtgaggcttg cataatggca ttcagaatga gggatctagt
     3001 gatgaagaat gaacatcccg cgttcttccc tccgaacagg acgatattgt aaattcactt
     3061 aattacgagg gcattgcagt aattgagttg cagttttacc actttcctga cagtgacaga
     3121 ctgcgtgttg gctctgtcac agactaaata gtttgaatga ttagcagtta tggtgatcag
     3181 tcaaccacca gggaataatc cttcatatta ttatcgtgct tcaccaacgc tgcctcaatt
     3241 gctctgaatg cttccagaga caccttatgt tctatacatg caattacaac atcagggtaa
     3301 ctcatagaaa tggtgctatt aagcatattt tttacacgaa tcagatccac ggagggatca
     3361 tcagcagatt gttctttatt cattttgtcg ctccatgcgc ttgctcttca tctagcggtt
     3421 aaaatattac ttcaaatctt tctgtatgaa gatttgagca cgttggcctt acatacatct
     3481 gtcggttgta tttccctcca gaatgccagc aggaccgcac tttgttacgc aaccaatact
     3541 attaagtgaa aacattccta atatttgaca taaatcatca acaaaacaca aggaggtcag
     3601 accagattga aacgataaaa acgataatgc aaactacgcg ccctcgtatc acatggaagg
     3661 ttttaccaat ggctcaggtt gccattttta aagaaatatt cgatcaagtg cgaaaagatt
     3721 tagactgtga attgttttat tctgaactaa aacgtcacaa cgtctcacat tatatttact
     3781 atctagccac agataatatt cacatcgtgt tagaaaacga taacaccgtg ttaataaaag
     3841 gacttaaaaa ggttgtaaat gttaaattct caagaaacac gcatcttata gaaacgtcct
     3901 atgataggtt gaaatcaaga gaaatcacat ttcagcaata cagggaaaat cttgctaaag
     3961 caggagtttt ccgatgggtt acaaatatcc atgaacataa aagatattac tatacctttg
     4021 ataattcatt actatttact gagagcattc agaacactac acaaatcttt ccacgctaaa
     4081 tcataacgtc cggtttcttc cgtgtcagca ccggggcgtt ggcataatgc aatacgtgta
     4141 cgcgctaaac cctgtgtgca tcgttttaat tattcccgga cactcccgca gagaagttcc
     4201 ccgtcagggc tgtggacata gttaatccgg gaatacaatg acgattcatc gcacctgaca
     4261 tacattaata aatattaaca atatgaaatt tcaactcatt gtttagggtt tgtttaattt
     4321 tctacacata cgattctgcg aacttcaaaa agcatcggga ataacaccat gaaaaaaatg
     4381 ctactcgcta ctgcgctggc cctgcttatt acaggatgtg ctcaacagac gtttactgtt
     4441 caaaacaaac cggcagcagt agcaccaaag gaaaccatca cccatcattt cttcgtttct
     4501 ggaattgggc agaagaaaac tgtcgatgca gccaaaattt gtggcggcgc agaaaatgtt
     4561 gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat tactttaggc
     4621 atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga gttgcccatc
     4681 gatatgggca actctatctg cactgctcat taatatactt ctgggttcct tccagttgtt
     4741 tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg gtgtctgcca
     4801 gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc actgactgac
     4861 agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc agagcatcat
     4921 tttcagcttt agcatcagct aactccttcg tgtattttgc atcgagcgca gcaacatcac
     4981 gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct ctggcatttt
     5041 tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca gcccatgaca
     5101 ggcagacgat gatgcagata accagagcgg agataatcgc ggtgactctg ctcatacatc
     5161 aatctctctg accgttccgc ccgcttcttt gaattttgca atcaggctgt cagccttatg
     5221 ctcgaactga ccataaccag cgcccggcag tgaagcccag atattgctgc aacggtcgat
     5281 tgcctgacgg atatcaccac gatcaatcat aggtaaagcg ccacgctcct taatctgctg
     5341 caatgccaca gcgtcctgac ttttcggaga gaagtctttc aggccaagct gcttgcggta
     5401 ggcatcccac caacgggaaa gaagctggta gcgtccggcg cctgttgatt tgagttttgg
     5461 gtttagcgtg acaagtttgc gagggtgatc ggagtaatca gtaaatagct ctccgcctac
     5521 aatgacgtca taaccatgat ttctggggat ctagcctgca gtaatagagc ggcccaggat
     5581 gcggagttgg gaaatcacct gagatgagat catgcgctgt gatgagcgtg caatgttggc
     5641 aggcagaccg aagaaggatg gcttatcgtc ttctggaatc ttctcaatga cagccctgta
     5701 atccagaatg ctgcagcttt gtggcaggct cacgctgtag ggaaagatgg acttcttgtt
     5761 gcgctgattg aacacgtcaa tgacgctaga gttgaagaat tgcttcaaat agctctgcag
     5821 gacgcgcaag tcgaaatagt tatcgatgcg gcctccgtag atggcgttct ccagcagacc
     5881 atggacaaac tcccattgca cgtccttggc accatcaaag agcctgtcaa tgatattgta
     5941 accggccctc aggtcggaga gagagaactc gtagaatttg gtccaacctt gaggaatgta
     6001 gttgcgcctc tcttggcatg cggcgtgaaa ccaggccaga gagaacagag catgtgcgcg
     6061 gtgagtgtta tctttcttag agatttgttc aggtgtccaa gattcatatg tgcgcatgag
     6121 attcttcttg agtccaggtg gtgattcata agtgatcttg aggcttgact ggaggaggat
     6181 aggtgtaaag tttgggtgca cttcggctgt caaccagagc ctgaaagtgt ctttgggttg
     6241 caaggtgttc agttccttct ccaagacagg cagccaggag acaaccaggt gcaagttctt
     6301 caaacacagc cagtcaccat tccttgcgca ttccttgagc atttggatag ccaagtcagc
     6361 ttggccctgg cccattgcaa cctggtggta acattctcct gatctttcgg cgttggcgag
     6421 ctcctggagt tcctgggagg gatcggctcc tggagaaata atgatcagaa tgggctcgat
     6481 ttcgagtgtc tccttgtaca gcctcttgag attgagtggg agtggagaga cttccttcaa
     6541 acccaaggtc ttgcaggcaa agagggccat agctgactgc aatctatctg gcctgagcac
     6601 ctgcacgacg aggatctgct ggaacaagct gaccttctta gccaagatgc taggaaattc
     6661 ctgctcacac atgctgttgt tgtagtaggt gcgccacaaa gcggcgtcct cgaaacacag
     6721 agtctggtac agggaaggca gagcgatctt caaggttgcc acagcccagg atctctcttg
     6781 atcgatccat gaaggcaact ggtccctgat cttctgctgg gagtcggctt tgcgcaacat
     6841 gtctccgacc accacgccag tgaaggtatc ccattcgttc tcctggaaca attctggatg
     6901 catacctctg acgaaatgca gggcgaacat cagctggtcg gccttgaaca ggcagcggca
     6961 gatgtattcg tacaccatgt gctgcaggga gctgatcagg gattgaatcc tctgttctgt
     7021 gttctcagaa tcctgcttat tctggagtgc cctttgaaac aacctcagaa aggcggccag
     7081 ggagaacctg tacatattat tgatcttgga gaggtcagag ataatgaagt acatctttga
     7141 agcagactca gcgagtggca gatatgcgtc gcgctcctgg tcgagagaga tttgcagctt
     7201 ataagattcc ttcagagact cttggatgag ggcggaagat gctttagtct gattcagaga
     7261 ctcgatcagg tccttgttct ccaggatgtt gccctgagaa gtagccaatg tctccagcag
     7321 agattcttcg agtttggcca gttggatctt cttatcctcc tcctgttgga gcagcttagt
     7381 cttctgttct tccaaatcgg gtttctcatg ttggatggtg agggccagga gctggcctct
     7441 cagtcctgag cgggtggttg tgaaattaac ctctgtgacg atgctggcag cgtctggtgg
     7501 aatgaatggg ttgggattcc tagtgctcag gaacaggcga aactcctcgt tatagtcgat
     7561 gatcttatct ccaatctgga ccacatagcg tgggccttgg gccaccaaat ctctgcgcaa
     7621 cagagggtac agaactggct ccacgccatc catctcctgg atgatcaagg tcttgccgaa
     7681 gcggacggcg agttccaggg cagtgatgaa gttggagtct tgctggttga tcacttccag
     7741 cctggaatcc ttcaagtgag tcttcagcca ctcagtagcc tgtgagctgg gatcgatgag
     7801 gaaagggcaa acgcgggatt gcaagataac cagtgcgttc tcgattgaga ggtcatcgct
     7861 gggcagacct tcagacttcc agatcagctg ctctgactcg gtgcagagaa atcttctcag
     7921 gtcaaacttc tccaggccgg cagacttggt ccactcctcc agacatgtct ttctgagaga
     7981 ctcaggtgca gcggacaagt aggtgataaa ggctgcggcc aactgagccc tctttgggag
     8041 ggttgccagc tcctcagtaa tctcgacgac ctgagcattc catctcttgt gttcgcggtc
     8101 cggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8401 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     8461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10621 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10681 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10741 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10801 ggtgtcgggg ctggcttaa
//
