LOCUS       sequence-217061-        5769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pScaf phagemid with insert for generating DNA origami scaffold 3024 
            bases in length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds.
  JOURNAL   Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 
            2018 Aug 9.
  PUBMED    30984875
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: " 
            10.1093/synbio/ysy015. Epub 2018 Aug 9"
COMMENT     Sequence Label: pScaf-3024.1
FEATURES             Location/Qualifiers
     source          1..5769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      44..424
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3403..3419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3403..3419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3416..3438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3427..3443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3451..3457,3458..3475,3476..3481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3496..3517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3634..3651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3805..4393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3885..3904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4564..5355,5356..5424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5187..5206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5425..5529)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5597..5615
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5653..5675)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc tacgaagagt tccagcaggg attccaagaa atggccaatg aagattggat
      481 cacctttcgc actaagacct acttgtttga ggagtttctg atgaattggc acgaccgcct
      541 caggaaagtg gaggagcatt ctgtgatgac tgtcaagctc caatctgagg tggacaaata
      601 taagattgtt atccctatcc tgaagtacgt ccgcggagaa cacctgtcac ccgatcactg
      661 gctggatctg ttccgcttgc tgggtctgcc tcgcggcaca tctctggaga aactgctgtt
      721 cggtgacctg ctgagagttg ccgataccat cgtggccaag gctgctgacc tgaaagatct
      781 gaactcacgc gcccagggtg aagtgaccat ccgcgaagca ctcagggaac tggatttgtg
      841 gggcgtgggt gctgtgttca cactgatcga ctatgaggac tcccagagcc gcaccatgaa
      901 gctgatcaag gattggaagg acatcgtcaa ccaggtgggc gacaatagat gcctcctgca
      961 gtccttgaag gactcaccat actataaagg ctttgaagac aaggtcagca tctgggaaag
     1021 gaaactcgcc gaactggacg aatatttgca gaacctcaac catattcaga gaaagtgggt
     1081 ttacctcgaa ccaatctttg gtcgcggagc cctgcccaaa gagcagacca gattcaacag
     1141 ggtggatgaa gatttccgca gcatcatgac agatatcaag aaggacaatc gcgtcacaac
     1201 cttgactacc cacgcaggca ttcgcaactc actgctgacc atcctggacc aattgcagag
     1261 atgccagcgc agcctcaacg agttcctgga ggagaagcgc agcgccttcc ctcgcttcta
     1321 cttcatcgga gacgatgacc tgctggagat cttgggccag tcaaccaatc catccgtgat
     1381 tcagtctcac ctcaagaagc tgtttgctgg tatcaactct gtctgtttcg atgagaagtc
     1441 taagcacatt actgcaatga agtccttgga gggagaagtt gtgccattca agaataaggt
     1501 gcccttgtcc aataacgtcg aaacctggct gaacgatctg gccctggaga tgaagaagac
     1561 cctggagcag ctgctgaagg agtgcgtgac aaccggacgc agctctcagg gagctgtgga
     1621 cccttctctg ttcccatcac agatcctgtg cttggccgaa cagatcaagt ttaccgaaga
     1681 tgtggagaac gcaattaaag atcactccct gcaccagatt gagacacagc tggtgaacaa
     1741 attggagcag tatactaaca tcgacacatc ttccgaggac ccaggtaaca cagagtccgg
     1801 tattctggag ctgaaactga aagcactgat tctcgacatt atccataaca tcgacgtggt
     1861 caagcagctg aaccaaatcc aagtgcacac caccgaagat tgggcctgga agaagcagtt
     1921 gaggttctac atgaagtccg accacacctg ttgcgttcag atggttgaca gcgagttcca
     1981 gtacacctat gagtaccaag gaaatgccag caagctcgtt tacactccac tcactgacaa
     2041 gtgttacctc accttgacac aggctatgaa gatgggcctg ggaggcaacc catacggtcc
     2101 agctggcact ggtaagacag agagcgttaa ggcactcgga ggtctgctgg gcaggcaggt
     2161 cctcgtgttc aactgtgatg aaggaatcga cgttaagtcc atgggaagaa tctttgttgg
     2221 cctcgttaag tgtggagctt ggggttgctt cgacgagttc aacaggctgg aggaatctgt
     2281 gctgagcgcc gtctctatgc agatccagac catccaggac gcattgaaga accacaggac
     2341 cgtctgcgag ctgttgggta aggaagtgga ggtgaactcc aactccggaa tcttcatcac
     2401 aatgaatccc gcaggtaaag gatatggagg aagacagaaa ctcccagaca acctgaagca
     2461 gctgttccgc ccagtggcta tgtcccatcc agacaatgag ctgatcgccg aagtcatcct
     2521 ctattccgag ggattcaaag atgctaaagt tctctccaga aagctcgtgg ccatcttcaa
     2581 tctgtcaaga gaactcctga cacctcagca gcattacgac tggggtctga gagccctcaa
     2641 gaccgtcctg agaggttcag gaaatctcct caggcagctg aacaagagcg gtacaacaca
     2701 gaatgcaaat gagagccaca ttgtcgtcca ggctctgagg ctgaatacca tgtcaaagtt
     2761 cacattcaca gactgcacaa gatttgacgc tctgattaaa gatgtgttcc ctggtattga
     2821 actcaaagaa gtggagtatg acgagctgag cgccgctttg aagcaggtgt ttgaggaggc
     2881 taactatgag attatcccta atcagatcaa gaaagcattg gaactgtatg aacagctgtg
     2941 tcagaggatg ggagtggtga ttgtgggccc atcaggcgca ggtaagagca ctctctggag
     3001 aatgctgaga gcagcactgt gcaagactgg aaaggtggtg aagcaataca ccatgaatcc
     3061 cggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     3301 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3361 aatagtggac tcttgttcca aactggagaa ttcgtaatca tggtcatagc tgtttcctgt
     3421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5521 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     5581 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     5641 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     5701 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5761 ctggcttaa
//
