LOCUS       sequence-217067-        8289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For tetracycline-inducible expression of myc-BirA*-NES-mCE Delta-NLS
            (""myc-BirA*-cCE"") in mammalian cells. For BioID studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
  TITLE     RNA-binding proteins and heat-shock protein 90 are constituents of 
            the cytoplasmic capping enzyme interactome.
  JOURNAL   J Biol Chem. 2018 Oct 26;293(43):16596-16607. doi: 
            10.1074/jbc.RA118.004973. Epub 2018 Aug 30.
  PUBMED    30166341
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004973"; journalName: "J Biol Chem"; date: 
            "2018-10-26- 26"; volume: "293"; issue: "43"; pages: "16596-16607"
COMMENT     Sequence Label: pcDNA3.1/TO-myc-BirA*-cCE
FEATURES             Location/Qualifiers
     source          1..8289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    612..630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    633..651
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     695..714
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        695..713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      731..740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             740..769
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(773..1120,1121..1123,1124..1732)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1745..1771
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(3672..3689)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3678..3902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3948..4376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4035..4054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4245..4266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4385..4405)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4390..4719
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4570..4705
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4632..4651
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4786..5580
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4840..4859)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5450..5469
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5754..5875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5791..5810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5845..5864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5924..5940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5924..5940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5937..5959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5948..5964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5972..5978,5979..5996,5997..6002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6017..6038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6155..6172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6326..6914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6406..6425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7085..7876,7877..7945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7708..7727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7946..8050)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8125..8144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atctggctaa
      661 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
      721 aagctggcta gccaccatgg aacaaaaact catctcagaa gaggatctcg acaaggacaa
      781 caccgtgccc ctgaagctga tcgccctgct ggccaacggc gagttccact ctggcgagca
      841 gctgggagag accctgggaa tgagcagagc cgccatcaac aagcacatcc agacactgag
      901 agactgggga gtggacgtgt tcaccgtgcc tggcaagggc tacagcctgc ctgagcctat
      961 ccagctgctg aacgccaagc agatcctggg acagctggat ggcggaagcg tggccgtgct
     1021 gcctgtgatc gactccacca atcagtacct gctggacaga atcggagagc tgaagtccgg
     1081 cgacgcctgc atcgccgagt accagcaggc tggcagagga ggcagaggac ggaagtggtt
     1141 cagcccattc ggagccaacc tgtacctgtc catgttctgg agactggagc agggacctgc
     1201 tgctgccatc ggactgagtc tggtgatcgg aatcgtgatg gccgaggtgc tgagaaagct
     1261 gggagccgac aaggtgagag tgaagtggcc taatgacctg tacctccagg accgcaagct
     1321 ggctggcatc ctggtggagc tgacaggcaa gacaggcgat gccgctcaga tcgtgatcgg
     1381 agccggaatc aacatggcca tgagaagagt ggaggagagc gtggtgaacc agggctggat
     1441 caccctgcag gaggctggca tcaacctgga ccggaacacc ctggccgcca tgctgatcag
     1501 agagctgaga gccgctctgg agctgttcga gcaggaggga ctggctcctt acctgagcag
     1561 atgggagaag ctggacaact tcatcaacag acctgtgaag ctgatcatcg gcgacaagga
     1621 aatcttcggc atctccagag gaatcgacaa gcagggagct ctgctgctgg agcaggacgg
     1681 aatcatcaag ccctggatgg gcggagaaat ctccctgaga agcgcagaga agctcgagct
     1741 tcagctacca ccgcttgaga gacttactct tgatatggcc atggaggccg cttacaacaa
     1801 gatcccgccg cggtggctga actgtccgcg gcgcggtcag ccggtggcag gaagattctt
     1861 acctctgaag acgatgctgg ggccaagata tgatagtcaa gttgctgaag aaaaccggtt
     1921 ccatcccagt atgctctcaa attatctaaa gagcctgaag gttaaaatga gcttgttggt
     1981 ggatctgaca aatacttcaa ggttctatga cagaaatgac atagaaaaag aaggaatcaa
     2041 gtatatcaaa cttcagtgta aaggacatgg cgaatgcccc accactgaga atactgagac
     2101 attcattcgc ctgtgtgaga ggtttaatga aagaagccca cctgaactta taggtgttca
     2161 ctgcacccat ggcttcaacc gcactggttt ccttatatgt gcctttttgg tggaaaaaat
     2221 ggattggagt attgaagcag cagttgctac atttgcccaa gccagaccac caggaatcta
     2281 caagggtgat tatttgaagg aactttttcg tcgctatgga gatatagaag aagcaccacc
     2341 accacctgta ttgccagatt ggtgttttga agatgaggat gaagaagatg aggatgaaga
     2401 tggaaaaaag gactcagaac cagggtcaag tgcatccttt agtaaaagga gaaaagagcg
     2461 gttaaaactg ggagctattt tcttggaagg cataactgtt aaaggtgtaa ctcaagtaac
     2521 aactcaaccc aagttaggag aggtgcagca gaaatgtcat cagttctgtg gctgggaagg
     2581 gtctgggttc cctggagcac agcctgtctc catggacaag caaaatatta gacttttaga
     2641 gcagaagcct tataaagtaa gctggaaagc agacggcact cgttacatga tgttgattga
     2701 tggcacaaat gaagttttta tgattgacag agataattcc gtttttcatg tttcaaatct
     2761 ggaatttcca tttcgtaaag atcttcgcat gcatttatca aatactcttt tggatgggga
     2821 aatgatcatt gacaaagtaa atggacaggc cgtcccaaga tatttgatct atgacataat
     2881 taagttcaat gcacaaccag ttggagactg tgattttaat attcgtctac agtgtattga
     2941 acgtgaaatt ataagtccac gacatgaaaa aatgaagact ggactcattg acaaaacaca
     3001 ggaaccattt agtgtcagac ctaaacaatt ttttgacatc aatatttcaa gaaagcttct
     3061 ggaaggaaat tttgccaaag aagtcagcca tgaaatggat ggacttattt ttcagcctat
     3121 tggaaaatac aagcctggtc gatgtgatga cattttgaaa tggaaacctc ccagtctaaa
     3181 ctctgtggat tttcgactta agataacaag aatgggagga gaagggttgc ttccacagaa
     3241 tgttggcctt ctctatgttg gaggttatga aagacccttt gcacaaatca aggtgacaaa
     3301 agaactaaaa cagtatgaca acaaaattat agaatgcaaa tttgagaaca atagctgggt
     3361 cttcatgaga cagaggatag acaaaagttt cccaaatgcc tacaacacag ccatggctgt
     3421 gtgcaatagc atctcgaacc ctgtcaccaa ggagatgctg tttgaattca ttgacagatg
     3481 tgcagcagcc gcccagggac agcccctgga ccctgacacg gagctcatgc cgcccccacc
     3541 gcccaaaaga ctgcatcggc caacctgatg actcgagtgt acaccactgt gctggatatc
     3601 tgcagaattc caccacactg gactagtgga tccgagctcg gtaccaagct taagtttaaa
     3661 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3721 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3781 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3841 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3901 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3961 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4021 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4081 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4141 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4201 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4261 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4321 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4621 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4741 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4801 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4861 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4921 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4981 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5041 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5101 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5161 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5221 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5281 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5341 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5401 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5461 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5521 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5581 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5641 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5701 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5881 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5941 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6001 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7081 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7141 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7201 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7261 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7321 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7381 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7441 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7501 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7561 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7621 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7681 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7741 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7801 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7861 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7921 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7981 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8041 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8101 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8161 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8221 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8281 gctgcttcg
//
