LOCUS       sequence-217074-       16146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of SAD B19 L and 
            mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16146)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 16146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pLV-TTBL
FEATURES             Location/Qualifiers
     source          1..16146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2547..2565,2566..2588,2589..2607,2608..2630,2631..2649,
                     2650..2672,2673..2691,2692..2714,2715..2733,2734..2756,
                     2757..2775,2776..2798,2799..2817)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2850..2874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2960..3580
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             3734..3790
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3791..4489
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4499..4555
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6200..6211
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10949..11537
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11002..11022)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11420..11431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11947..12127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12153..12170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12159..12383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12429..12857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12516..12535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12726..12747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12866..12886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12871..13201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13052..13187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13114..13133
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13249..13296
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13315..13689
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13819..13940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13856..13875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13910..13929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13989..14005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13989..14005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14002..14024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14013..14029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14037..14043,14044..14061,14062..14067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14082..14103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14220..14237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14391..14979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14471..14490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15150..15941,15942..16010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15773..15792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16011..16115)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcgt tggctcgacc
     2521 cgggtaccga gctcgacttt cacttttctc tatcactgat agggagtggt aaactcgact
     2581 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
     2641 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
     2701 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
     2761 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
     2821 gtaaaggatc cctcgaccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     2881 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     2941 ggccctgcag gccgccacca tgtccagact ggacaagagc aaagtcatca actctgcctt
     3001 ggagctcctg aatgaagttg gcattgaggg cttgaccacc aggaagctgg cccagaagct
     3061 gggtgtggag cagcctaccc tgtactggca tgtgaagaac aagagggctc tgcttgatgc
     3121 cctggccatt gagatgttgg acaggcacca cacccacttc tgccctctgg aaggggagtc
     3181 ctggcaggac ttcctgagga acaatgccaa gagcttcaga tgtgccttgc tctcccaccg
     3241 ggatggtgcc aaagttcact tgggcaccag gcctacagag aagcagtatg agaccctgga
     3301 gaaccagctg gcattcctgt gccaacaagg cttctccctg gaaaatgcct tgtatgccct
     3361 ctctgctgtg ggccacttca ccttgggctg tgtgctggag gaccaggagc accaagttgc
     3421 caaggaggag agggagaccc ccaccactga ctccatgcca ccactgctgc ggcaagctat
     3481 tgagttgttt gaccaccaag gggctgagcc tgcattcctt tttggcctgg aactgatcat
     3541 ctgtggcctg gaaaagcagc tgaaatgtga gtctggctct cacgtgccca aaaagagaaa
     3601 gcacgtgcct gctgatgcct tggatgattt tgacctggac atgctgcctg ctgatgccct
     3661 ggatgacttt gatttggaca tgctccctgc tgatgcactt gatgattttg acctggatat
     3721 gctgggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa
     3781 ccctggacct atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     3841 caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     3901 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     3961 cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc
     4021 cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     4081 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     4141 caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac
     4201 actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag
     4261 aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac
     4321 atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta
     4381 cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc
     4441 agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc
     4501 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgct
     4561 cgatcctgga gaggtctatg atgaccctat tgacccaatc gagttagagg ctgaacccag
     4621 aggaaccccc attgtcccca acatcttgag gaactctgac tacaatctca actctccttt
     4681 gatagaagat cctgctagac taatgttaga atggttaaaa acagggaata gaccttatcg
     4741 gatgactcta acagacaatt gctccaggtc tttcagagtt ttgaaagatt atttcaagaa
     4801 ggtagatttg ggttctctca aggtgggcgg aatggctgca cagtcaatga tttctctctg
     4861 gttatatggt gcccactctg aatccaacag gagccggaga tgtataacag acttggccca
     4921 tttctattcc aagtcgtccc ccatagagaa gctgttgaat ctcacgctag gaaatagagg
     4981 gctgagaatc cccccagagg gagtgttaag ttgccttgag agggttgatt atgataatgc
     5041 atttggaagg tatcttgcca acacgtattc ctcttacttg ttcttccatg taatcacctt
     5101 atacatgaac gccctagact gggatgaaga aaagaccatc ctagcattat ggaaagattt
     5161 aacctcagtg gacatcggga aggacttggt aaagttcaaa gaccaaatat ggggactgct
     5221 gatcgtgaca aaggactttg tttactccca aagttccaat tgtctttttg acagaaacta
     5281 cacacttatg ctaaaagatc ttttcttgtc tcgcttcaac tccttaatgg tcttgctctc
     5341 tcccccagag ccccgatact cagatgactt gatatctcaa ctatgccagc tgtacattgc
     5401 tggggatcaa gtcttgtcta tgtgtggaaa ctccggctat gaagtcatca aaatattgga
     5461 gccatatgtc gtgaatagtt tagtccagag agcagaaaag tttaggcctc tcattcattc
     5521 cttgggagac tttcctgtat ttataaaaga caaggtaagt caacttgaag agacgttcgg
     5581 tccctgtgca agaaggttct ttagggctct ggatcaattc gacaacatac atgacttggt
     5641 ttttgtgttt ggctgttaca ggcattgggg gcacccatat atagattatc gaaagggtct
     5701 gtcaaaacta tatgatcagg ttcaccttaa aaaaatgata gataagtcct accaggagtg
     5761 cttagcaagc gacctagcca ggaggatcct tagatggggt tttgataagt actccaagtg
     5821 gtatctggat tcaagattcc tagcccgaga ccaccccttg actccttata tcaaaaccca
     5881 aacatggcca cccaaacata ttgtagactt ggtgggggat acatggcaca agctcccgat
     5941 cacgcagatc tttgagattc ctgaatcaat ggatccgtca gaaatattgg atgacaaatc
     6001 acattctttc accagaacga gactagcttc ttggctgtca gaaaaccgag gggggcctgt
     6061 tcctagcgaa aaagttatta tcacggccct gtctaagccg cctgtcaatc cccgagagtt
     6121 tctgaggtct atagacctcg gaggattgcc agatgaagac ttgataattg gcctcaagcc
     6181 aaaggaacgg gaattgaaga ttgaaggtcg attctttgct ctaatgtcat ggaatctaag
     6241 attgtatttt gtcatcactg aaaaactctt ggccaactac atcttgccac tttttgacgc
     6301 gctgactatg acagacaacc tgaacaaggt gtttaaaaag ctgatcgaca gggtcaccgg
     6361 gcaagggctt ttggactatt caagggtcac atatgcattt cacctggact atgaaaagtg
     6421 gaacaaccat caaagattag agtcaacaga ggatgtattt tctgtcctag atcaagtgtt
     6481 tggattgaag agagtgtttt ctagaacaca cgagtttttt caaaaggcct ggatctatta
     6541 ttcagacaga tcagacctca tcgggttacg ggaggatcaa atatactgct tagatgcgtc
     6601 caacggccca acctgttgga atggccagga tggcgggcta gaaggcttac ggcagaaggg
     6661 ctggagtcta gtcagcttat tgatgataga tagagaatct caaatcagga acacaagaac
     6721 caaaatacta gctcaaggag acaaccaggt tttatgtccg acatacatgt tgtcgccagg
     6781 gctatctcaa gaggggctcc tctatgaatt ggagagaata tcaaggaatg cactttcgat
     6841 atacagagcc gtcgaggaag gggcatctaa gctagggctg atcatcaaga aagaagagac
     6901 catgtgtagt tatgacttcc tcatctatgg aaaaacccct ttgtttagag gtaacatatt
     6961 ggtgcctgag tccaaaagat gggccagagt ctcttgcgtc tctaatgacc aaatagtcaa
     7021 cctcgccaat ataatgtcga cagtgtccac caatgcgcta acagtggcac aacactctca
     7081 atctttgatc aaaccgatga gggattttct gctcatgtca gtacaggcag tctttcacta
     7141 cctgctattt agcccaatct taaagggaag agtttacaag attctgagcg ctgaagggga
     7201 gagctttctc ctagccatgt caaggataat ctatctagat ccttctttgg gagggatatc
     7261 tggaatgtcc ctcggaagat tccatatacg acagttctca gaccctgtct ctgaagggtt
     7321 atccttctgg agagagatct ggttaagctc ccaagagtcc tggattcacg cgttgtgtca
     7381 agaggctgga aacccagatc ttggagagag aacactcgag agcttcactc gccttctaga
     7441 agatccgacc accttaaata tcagaggagg ggccagtcct accattctac tcaaggatgc
     7501 aatcagaaag gctttatatg acgaggtgga caaggtggaa aattcagagt ttcgagaggc
     7561 aatcctgttg tccaagaccc atagagataa ttttatactc ttcttaatat ctgttgagcc
     7621 tctgtttcct cgatttctca gtgagctatt cagttcgtct tttttgggaa tccccgagtc
     7681 aatcattgga ttgatacaaa actcccgaac gataagaagg cagtttagaa agagtctctc
     7741 aaaaacttta gaagaatcct tctacaactc agagatccac gggattagtc ggatgaccca
     7801 gacacctcag agggttgggg gggtgtggcc ttgctcttca gagagggcag atctacttag
     7861 ggagatctct tggggaagaa aagtggtagg cacgacagtt cctcaccctt ctgagatgtt
     7921 gggattactt cccaagtcct ctatttcttg cacttgtgga gcaacaggag gaggcaatcc
     7981 tagagtttct gtatcagtac tcccgtcctt tgatcagtca tttttttcac gaggccccct
     8041 aaagggatac ttgggctcgt ccacctctat gtcgacccag ctattccatg catgggaaaa
     8101 agtcactaat gttcatgtgg tgaagagagc tctatcgtta aaagaatcta taaactggtt
     8161 cattactaga gattccaact tggctcaagc tctaattagg aacattatgt ctctgacagg
     8221 ccctgatttc cctctagagg aggcccctgt cttcaaaagg acggggtcag ccttgcatag
     8281 gttcaagtct gccagataca gcgaaggagg gtattcttct gtctgcccga acctcctctc
     8341 tcatatttct gttagtacag acaccatgtc tgatttgacc caagacggga agaactacga
     8401 tttcatgttc cagccattga tgctttatgc acagacatgg acatcagagc tggtacagag
     8461 agacacaagg ctaagagact ctacgtttca ttggcacctc cgatgcaaca ggtgtgtgag
     8521 acccattgac gacgtgaccc tggagacctc tcagatcttc gagtttccgg atgtgtcgaa
     8581 aagaatatcc agaatggttt ctggggctgt gcctcacttc cagaggcttc ccgatatccg
     8641 tctgagacca ggagattttg aatctctaag cggtagagaa aagtctcacc atatcggatc
     8701 agctcagggg ctcttatact caatcttagt ggcaattcac gactcaggat acaatgatgg
     8761 aaccatcttc cctgtcaaca tatacggcaa ggtttcccct agagactatt tgagagggct
     8821 cgcaagggga gtattgatag gatcctcgat ttgcttcttg acaagaatga caaatatcaa
     8881 tattaataga cctcttgaat tggtctcagg ggtaatctca tatattctcc tgaggctaga
     8941 taaccatccc tccttgtaca taatgctcag agaaccgtct cttagaggag agatattttc
     9001 tatccctcag aaaatccccg ccgcttatcc aaccactatg aaagaaggca acagatcaat
     9061 cttgtgttat ctccaacatg tgctacgcta tgagcgagag ataatcacgg cgtctccaga
     9121 gaatgactgg ctatggatct tttcagactt tagaagtgcc aaaatgacgt acctatccct
     9181 cattacttac cagtctcatc ttctactcca gagggttgag agaaacctat ctaagagtat
     9241 gagagataac ctgcgacaat tgagttcttt gatgaggcag gtgctgggcg ggcacggaga
     9301 agatacctta gagtcagacg acaacattca acgactgcta aaagactctt tacgaaggac
     9361 aagatgggtg gatcaagagg tgcgccatgc agctagaacc atgactggag attacagccc
     9421 caacaagaag gtgtcccgta aggtaggatg ttcagaatgg gtctgctctg ctcaacaggt
     9481 tgcagtctct acctcagcaa acccggcccc tgtctcggag cttgacataa gggccctctc
     9541 taagaggttc cagaaccctt tgatctcggg cttgagagtg gttcagtggg caaccggtgc
     9601 tcattataag cttaagccta ttctagatga tctcaatgtt ttcccatctc tctgccttgt
     9661 agttggggac gggtcagggg ggatatcaag ggcagtcctc aacatgtttc cagatgccaa
     9721 gcttgtgttc aacagtcttt tagaggtgaa tgacctgatg gcttccggaa cacatccact
     9781 gcctccttca gcaatcatga ggggaggaaa tgatatcgtc tccagagtga tagatcttga
     9841 ctcaatctgg gaaaaaccgt ccgacttgag aaacttggca acctggaaat acttccagtc
     9901 agtccaaaag caggtcaaca tgtcctatga cctcattatt tgcgatgcag aagttactga
     9961 cattgcatct atcaaccgga tcaccctgtt aatgtccgat tttgcattgt ctatagatgg
    10021 accactctat ttggtcttca aaacttatgg gactatgcta gtaaatccaa actacaaggc
    10081 tattcaacac ctgtcaagag cgttcccctc ggtcacaggg tttatcaccc aagtaacttc
    10141 gtctttttca tctgagctct acctccgatt ctccaaacga gggaagtttt tcagagatgc
    10201 tgagtacttg acctcttcca cccttcgaga aatgagcctt gtgttattca attgtagcag
    10261 ccccaagagt gagatgcaga gagctcgttc cttgaactat caggatcttg tgagaggatt
    10321 tcctgaagaa atcatatcaa atccttacaa tgagatgatc ataactctga ttgacagtga
    10381 tgtagaatct tttctagtcc acaagatggt tgatgatctt gagttacaga ggggaactct
    10441 gtctaaagtg gctatcatta tagccatcat gatagttttc tccaacagag tcttcaacgt
    10501 ttccaaaccc ctaactgacc cctcgttcta tccaccgtct gatcccaaaa tcctgaggca
    10561 cttcaacata tgttgcagta ctatgatgta tctatctact gctttaggtg acgtccctag
    10621 cttcgcaaga cttcacgacc tgtataacag acctataact tattacttca gaaagcaagt
    10681 cattcgaggg aacgtttatc tatcttggag ttggtccaac gacacctcag tgttcaaaag
    10741 ggtagcctgt aattctagcc tgagtctgtc atctcactgg atcaggttga tttacaagat
    10801 agtgaagact accagactcg ttggcagcat caaggatcta tccagagaag tggaaagaca
    10861 ccttcatagg tacaacaggt ggatcaccct agaggatatc agatctagat catccctact
    10921 agactacagt tgcctgtgat gagctagcaa tcaacctctg gattacaaaa tttgtgaaag
    10981 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    11041 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    11101 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    11161 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    11221 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    11281 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    11341 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    11401 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    11461 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    11521 ttgggccgcc tccccgcctg ttcgaattcg agctcggtac ctttaagacc aatgacttac
    11581 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
    11641 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
    11701 cctgattgac agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    11761 tgctacaagc tagtaccagt tgagccagat aagatagaag aggccaataa aggagagaac
    11821 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    11881 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggac
    11941 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    12001 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    12061 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    12121 tctagcaggg ccgctttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
    12181 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    12241 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    12301 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    12361 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    12421 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    12481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    12541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
    12601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    12661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    12721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    12781 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
    12841 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
    12901 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    12961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    13021 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    13081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    13141 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    13201 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
    13261 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
    13321 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
    13381 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
    13441 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
    13501 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
    13561 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
    13621 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
    13681 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
    13741 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
    13801 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    13861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    13921 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    13981 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    14041 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    14101 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    14161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    14221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    14281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    14341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    14401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    14461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    14521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    14581 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    14641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    14701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    14761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    14821 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    14881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    14941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    15001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    15061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    15121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    15181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    15241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    15301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    15361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    15421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    15481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    15541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    15601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    15661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    15721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    15781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    15841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    15901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    15961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    16021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    16081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    16141 gacgtc
//
