LOCUS       sequence-217077-        1362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express in mammalian cells the endocytic protein Rab7a fused to 
            mEmerald by transfection or by generating AAV virions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Pantelis Tsoulfas
  TITLE     Eukaryotic expression plasmids for expression and visualization of 
            early endocytic markers Rab5 and Rab7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-UbC-mEmerald Rab7a
FEATURES             Location/Qualifiers
     source          1..1362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggactcagat ctcgagccat gacctctagg aagaaagtgt tgctgaaggt tatcatcctg
      781 ggagattctg gagtcgggaa gacatcactc atgaaccagt atgtgaataa gaaattcagc
      841 aatcagtaca aagccacaat aggagctgac tttctgacca aggaggtgat ggtggatgac
      901 aggctagtca caatgcagat atgggacaca gcaggacagg aacggttcca gtctctcggt
      961 gtggccttct acagaggtgc agactgctgc gttctggtat ttgatgtgac tgcccccaac
     1021 acattcaaaa ccctagatag ctggagagat gagtttctca tccaggccag tccccgagat
     1081 cctgaaaact tcccatttgt tgtgttggga aacaagattg acctcgaaaa cagacaagtg
     1141 gccacaaagc gggcacaggc ctggtgctac agcaaaaaca acattcccta ctttgagacc
     1201 agtgccaagg aggccatcaa cgtggagcag gcgttccaga cgattgcacg gaatgcactt
     1261 aagcaggaaa cggaggtgga gctgtacaac gaatttcctg aacctatcaa actggacaag
     1321 aatgaccggg ccaaggcctc ggcagaaagc tgcagttgct ga
//
