LOCUS       sequence-217086-        9783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of Sox10 (generated 
            from plasmid #20321).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: FUW-TetON-Sox10
FEATURES             Location/Qualifiers
     source          1..9783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        455..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     608..628
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             671..851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    898..1023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1516..1749
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1934..1978
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2276..2393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2446..2464,2465..2487,2488..2506,2507..2529,2530..2548,
                     2549..2571,2572..2590,2591..2613,2614..2632,2633..2655,
                     2656..2674,2675..2697,2698..2716)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2749..2786
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2783..2807
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4311..4899
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4364..4384)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4782..4793)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4902..4918)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4903..4919)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5424..5604
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5630..5647)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5636..5860
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5906..6334
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5993..6012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6203..6224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6343..6363)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6348..6677
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6528..6663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6590..6609
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6725..6772
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6791..7165
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7295..7416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7332..7351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7386..7405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7465..7481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7465..7481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7478..7500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7489..7505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7513..7519,7520..7537,7538..7543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7558..7579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7696..7713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7867..8455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7947..7966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8626..9417,9418..9486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9249..9268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9487..9591)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9666..9685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      661 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      841 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      901 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      961 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1021 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1081 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1141 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1201 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1261 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1321 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1381 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1441 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1501 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1561 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1621 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1681 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1741 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1801 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1861 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1921 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1981 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2041 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2101 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2161 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2221 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2401 attacaggga cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata
     2461 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2521 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2581 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2641 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2701 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagt aggcgtgtac
     2761 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2821 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg
     2881 aattcatggc ggaggagcag gacctatcgg aggtggagct gagccccgtg ggctcggagg
     2941 agccccgctg cctgtccccg gggagcgcgc cctcgctagg gcccgacggc ggcggcggcg
     3001 gatcgggcct gcgagccagc ccggggccag gcgagctggg caaggtcaag aaggagcagc
     3061 aggacggcga ggcggacgat gacaagttcc ccgtgtgcat ccgcgaggcc gtcagccagg
     3121 tgctcagcgg ctacgactgg acgctggtgc ccatgcccgt gcgcgtcaac ggcgccagca
     3181 aaagcaagcc gcacgtcaag cggcccatga acgccttcat ggtgtgggct caggcagcgc
     3241 gcaggaagct cgcggaccag tacccgcacc tgcacaacgc tgagctcagc aagacgctgg
     3301 gcaagctctg gaggctgctg aacgaaagtg acaagcgccc cttcatcgag gaggctgagc
     3361 ggctccgtat gcagcacaag aaagaccacc cggactacaa gtaccagccc aggcggcgga
     3421 agaacgggaa ggccgcccag ggcgaggcgg agtgccccgg tggggaggcc gagcaaggtg
     3481 ggaccgccgc catccaggcc cactacaaga gcgcccactt ggaccaccgg cacccaggag
     3541 agggctcccc catgtcagat gggaaccccg agcacccctc aggccagagc catggcccac
     3601 ccacccctcc aaccaccccg aagacagagc tgcagtcggg caaggcagac ccgaagcggg
     3661 acgggcgctc catgggggag ggcgggaagc ctcacatcga cttcggcaac gtggacattg
     3721 gtgagatcag ccacgaggta atgtccaaca tggagacctt tgatgtggct gagttggacc
     3781 agtacctgcc gcccaatggg cacccaggcc atgtgagcag ctactcagca gccggctatg
     3841 ggctgggcag tgccctggcc gtggccagtg gacactccgc ctggatctcc aagccaccag
     3901 gcgtggctct gcccacggtc tcaccacctg gtgtggatgc caaagcccag gtgaagacag
     3961 agaccgcggg gccccagggg cccccacact acaccgacca gccatccacc tcacagatcg
     4021 cctacacctc cctcagcctg ccccactatg gctcagcctt cccctccatc tcccgccccc
     4081 agtttgacta ctctgaccat cagccctcag gaccctatta tggccactcg ggccaggcct
     4141 ctggcctcta ctcggccttc tcctatatgg ggccctcgca gcggcccctc tacacggcca
     4201 tctctgaccc cagcccctca gggccccagt cccacagccc cacacactgg gagcagccag
     4261 tatatacgac actgtcccgg ccctaagaat tcgatatcaa gcttatcgat aatcaacctc
     4321 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4381 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4441 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4501 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4561 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4621 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4681 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4741 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4801 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4861 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     4921 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     4981 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     5041 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5101 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5161 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     5221 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     5281 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     5341 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     5401 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5581 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     5641 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5701 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5761 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5821 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5881 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5941 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6001 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6061 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6121 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6181 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6241 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6301 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6421 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6481 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6541 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6601 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6661 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     6721 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     6781 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     6841 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     6901 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     6961 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     7021 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     7081 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     7141 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     7201 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7261 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7441 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9601 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9661 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9721 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9781 gac
//
