LOCUS       sequence-217088-       15428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-inducible LIMD1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Ibar C, Kirichenko E, Keepers B, Enners E, Fleisch K, Irvine KD
  TITLE     Tension-dependent regulation of mammalian Hippo signaling through 
            LIMD1.
  JOURNAL   J Cell Sci. 2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700.
  PUBMED    29440237
REFERENCE   2  (bases 1 to 15428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700."
COMMENT     Sequence Label: pTRIPZ-EGFP:LIMD1
FEATURES             Location/Qualifiers
     source          1..15428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2993..2995,2996..2998,2999..3709)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2993..2995,2996..2998,2999..3709)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3038..3059)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3299..3318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3646..3667
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           5821..5914
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5940..6052
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(6147..6165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6178..7389
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7226..7245
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7402..7435)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7442..8149
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(8150..8183)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    8206..8778
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8373..8390)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8600..8619
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8781..9383
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9280..9300
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9399..9987
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9452..9472)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9870..9881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10194..10427
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10528..10545)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10534..10758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10804..11232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10891..10910)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11101..11122
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11241..11261)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11246..11575
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11426..11561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11488..11507
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12783..12904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12953..12969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12953..12969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12966..12988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12977..12993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13001..13007,13008..13025,13026..13031))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13046..13067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13184..13201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13355..13943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13435..13454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14114..14905,14906..14974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14737..14756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14975..15079)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    15127..15261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caatggtgag
     3001 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3061 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3121 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3181 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3241 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3301 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3361 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3421 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3481 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3541 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3601 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3661 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
     3721 cgagatggat aagtatgacg acctgggcct ggaggccagt aaattcatcg aggacctgaa
     3781 catgtatgag gcctctaagg atgggctctt ccgagtggac aagggtgcag gcaacaaccc
     3841 cgagtttgag gaaactcgca gggtgttcgc caccaagatg gccaaaatcc acctccagca
     3901 gcagcagcag cagctcctgc aggaggagac tctgcccagg gggagtagag gccctgtcaa
     3961 tggagggggc cgcctgggcc cacaggcccg ttgggaagtt gtgggcagca agctgactgt
     4021 ggatggtgct gccaagcctc ctcttgctgc ctcgacaggg gcacctgggg cagtcaccac
     4081 cctcgctgct gggcagcccc cgtacccacc gcaggagcag agatccaggc catacctgca
     4141 tggcacgagg catggcagcc aggactgtgg ttccagggag agcctggcga cttctgagat
     4201 gtctgctttc caccagccag gcccctgtga ggatccttcc tgcctcactc atggagacta
     4261 ttatgacaac ctctccttgg caagcccaaa gtggggtgac aaaccaggag tgtcccccag
     4321 catcggcctg agtgtaggga gtgggtggcc tagctccccg gggagtgacc caccactgcc
     4381 caaaccctgc ggggaccatc ccctaaatca ccgacagctc tccctgagct ccagcaggtc
     4441 ttctgagggt agcctcggtg gtcagaatag tggcattggt ggccgcagca gcgagaagcc
     4501 aacaggcctt tggtccactg cctcctccca gcgggtgagc cctggcctgc cttccccaaa
     4561 cttggagaac ggagcaccag ctgtggggcc tgttcagccc aggactcctt ctgtgtcagc
     4621 acccttggcc ctgagctgcc ccaggcaagg aggtcttcca agatcaaact cggggctggg
     4681 gggtgaggtt tcaggtgtga tgtccaaacc caatgtggac ccccaaccct ggttccagga
     4741 tgggcccaaa tcttaccttt ccagttctgc cccgtcatcc tcgccagctg gcctggacgg
     4801 ttcacagcag ggtgcggtcc ctgggctggg gccgaagcct ggctgcacag accttggcac
     4861 tggtcccaag ctcagcccca ccagtcttgt ccatccagtg atgtccaccc tgcctgagtt
     4921 atcttgtaaa gagggtcccc tgggctggtc ttctgatggt agcctgggat ctgtgctcct
     4981 ggacagcccc agctccccta gggtaaggct gccctgccag cccctcgtcc caggtcctga
     5041 gctgagaccc tctgctgctg agttgaaatt agaagccctc acccaacgtc tggagcgaga
     5101 gatggatgct cacccgaagg ctgattactt tggagcctgt gtgaaatgca gcaaaggggt
     5161 gtttggggct ggccaggcct gtcaggccat ggggaacctc taccatgaca catgcttcac
     5221 ctgtgcagct tgcagccgga agctgagagg aaaagccttt tattttgtca acggcaaagt
     5281 gttttgtgaa gaagacttcc tgtactctgg tttccagcag tcggctgaca ggtgttttct
     5341 ttgtggacat ctgatcatgg acatgatcct gcaagccctg gggaagtcct accaccccgg
     5401 ctgtttccgc tgtgtcatct gtaatgagtg tttggatggg gtgcccttca ccgtggactc
     5461 agagaacaag atctactgtg tccgagatta ccacaaggtg ctggccccca agtgtgcagc
     5521 ctgtgggctt cccatccttc cacctgaggg ctcagatgag accatccgtg tcgtgtccat
     5581 ggacagagac taccacgtgg agtgttacca ctgcgaggac tgtggtctgg agctcaatga
     5641 tgaagatggc caccgctgtt atccgctgga ggaccacctg ttctgtcact cctgccacgt
     5701 gaagaggctg gagaagagac cctcatctac agcccttcac cagcaccact tcagtctaga
     5761 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aatagtttgt
     5821 ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg
     5881 ggattacttc ttcaggttaa cccaacagaa ggctcgaggt aaccggatcc tgatcagaat
     5941 tcaaggggct actttaggag caattatctt gtttactaaa actgaatacc ttgctatctc
     6001 tttgatacat ttttacaaag ctgaattaaa atggtataaa ttaaatcact tttttcaatt
     6061 ggaagactaa tgcggccggc cattactccg tctcgtgtct tgttgcatat gtctgctggt
     6121 ttgtttgatg ttgtttgcgg gcgggcccta tagtgagtcg tattacctag gacgcgtggc
     6181 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     6241 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     6301 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     6361 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     6421 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     6481 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     6541 ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc
     6601 cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa
     6661 gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag
     6721 caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt
     6781 cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc
     6841 taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac
     6901 gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat
     6961 ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg
     7021 acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg
     7081 taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga
     7141 caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt
     7201 ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg
     7261 gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata
     7321 tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt
     7381 ttgttagacg ctagcggatc cataacttcg tatagtatac attatacgaa gttatgccac
     7441 catgtctagg ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt
     7501 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     7561 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     7621 ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg
     7681 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca
     7741 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     7801 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     7861 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     7921 acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca
     7981 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     8041 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     8101 cccagccgat gcccttgacg attttgacct tgacatgctc cccgggtaaa taacttcgta
     8161 tagtatacat tatacgaagt tatggatccg cggccgcaaa ttccgcccct ctccctcccc
     8221 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     8281 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     8341 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     8401 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     8461 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     8521 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     8581 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     8641 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     8701 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     8761 ttgaaaaaca cgataatacc atggccaccg agtacaagcc cacggtgcgc ctcgccaccc
     8821 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     8881 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     8941 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     9001 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggct
     9061 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     9121 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     9181 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     9241 gcgctggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     9301 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     9361 tgacccgcaa gcccggtgcc tgagttcgcg tctggaacaa tcaacctctg gattacaaaa
     9421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9481 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9541 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9601 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9661 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9721 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9781 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     9841 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9901 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9961 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
    10021 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
    10081 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
    10141 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
    10201 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
    10261 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10321 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10381 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
    10441 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
    10501 ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
    10561 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
    10621 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
    10681 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
    10741 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
    10801 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10861 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10921 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10981 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    11041 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    11101 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    11161 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    11221 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
    11281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
    11341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    11401 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    11461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
    11521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    11581 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact
    11641 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
    11701 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
    11761 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
    11821 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat taattcagcg
    11881 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
    11941 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
    12001 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
    12061 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
    12121 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
    12181 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
    12241 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
    12301 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
    12361 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
    12421 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
    12481 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
    12541 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
    12601 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc
    12661 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    12721 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    12781 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    12841 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12901 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    12961 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    13021 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    13081 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    13141 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    13201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    13261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    13321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    13381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    13441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    13501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    13561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    13621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    13681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    13741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    13801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    13861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    13921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    13981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    14041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    14101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    14161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    14221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    14281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    14341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    14401 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    14461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    14521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    14581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    14641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    14701 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    14761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    14821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    14881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    14941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    15001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    15061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    15121 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    15181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    15241 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    15301 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    15361 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    15421 ttagtagt
//
