LOCUS       sequence-217097-       10586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  prp8-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Umen JG, Guthrie C
  TITLE     Mutagenesis of the yeast gene PRP8 reveals domains governing the 
            specificity and fidelity of 3' splice site selection.
  JOURNAL   Genetics. 1996 Jun;143(2):723-39.
  PUBMED    8725222
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1996-06"; volume: "143(2)"; pages: "723-39"
COMMENT     Sequence Label: pRS303 prp8-1
FEATURES             Location/Qualifiers
     source          1..10586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     93..110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    227..248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(263..268,269..286,287..293)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    301..317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     306..328
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     325..341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     325..341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        362..380
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6641..6660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6642..6660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6667..6684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6667..6683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6676..6698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6824..7279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6911..6930)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7121..7142
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7539..8201)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8202..8389)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8536..8555)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8655..8677
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8715..8733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8801..8905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8906..8974,8975..9766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9124..9143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9937..10525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10426..10445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
       61 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
      121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
      181 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      241 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      301 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg
      361 aaattaaccc tcactaaagg gaacaaaagc tggtaccggg ccccccctcg aggtcgacaa
      421 ggacacaata tgttaaattt attaatccac agaaagggcc taacttattt acatctcgac
      481 tacaatttca atttaaaacc aactaaaacc ttaaccacaa aagaacgtaa aaagtcaaga
      541 cttggaaact catttcattt gatgcgtgaa ttgctaaaaa tgatgaaact gattgtagat
      601 acacatgtgc agtttaggtt aggaaatgta gatgccttcc agttagcaga tggtatacat
      661 tatattttga accacatagg acaattgacc ggtatttatc gttataaata taaagtaatg
      721 catcaaatcc gcgcatgtaa agatctgaaa catatcatat attataagtt taataaaaat
      781 ttgggaaaag gacctggttg tggattttgg caacctgcgt ggagagtgtg gctaaatttc
      841 ttaagaggca ctataccact actcgaaaga tatattggaa accttatcac acgtcaattt
      901 gaaggccgtt ctaatgaaat tgtcaaaact acaactaaac aaagacttga cgcctactac
      961 gatcttgagt tacgaaactc ggtcatggat gacattttag aaatgatgcc tgagagtatt
     1021 cgacagaaaa aggcaagaac tatactgcaa catttaagtg aggcttggag atgttggaag
     1081 gctaatattc catgggatgt tcctggtatg ccagccccaa tcaaaaaaat aattgaacgg
     1141 tatataaaat ccaaagctga tgcctgggta tctgcagctc attacaatcg agaacgtata
     1201 aagcgtggag ctcacgtcga aaagactatg gttaaaaaaa atctggggcg tttaacgaga
     1261 ctttggatca aaaatgagca ggaacgtcaa aggcaaatac agaaaaacgg tcccgaaatt
     1321 actcccgaag aggctacgac catattttct gttatggttg agtggcttga atcaaggagc
     1381 ttttcaccaa taccatttcc acctttaact tacaaaaatg atactaagat attagttctt
     1441 gccctcgaag acctaaaaga tgtctacgca tcaaaagtac gtttaaatgc atctgaaagg
     1501 gaagaacttg cgttgataga ggaggcttat gataatcctc acgatacttt gaacagaatc
     1561 aaaaagtact tgttgaccca gcgagttttt aagcctgttg atataaccat gatggaaaac
     1621 tatcaaaaca tttcccctgt ttattcagtt gatcccttgg aaaagattac cgatgcatat
     1681 cttgatcagt atttatggta cgaagctgac caacgaaagc ttttcccaaa ctggattaaa
     1741 ccaagtgatt cagagatacc acctcttctg gtatacaagt ggactcaggg tataaacaac
     1801 ctatctgaaa tttgggatgt atccagaggc caatctgcag ttttgcttga gactactttg
     1861 ggtgaaatgg ccgaaaaaat tgactttact ttacttaata gattacttcg cctgattgta
     1921 gatcctaata ttgctgacta tatcactgca aaaaataatg ttgttatcaa ctttaaagat
     1981 atgagtcacg tcaacaaata tggcttaata cgcgggttga agttcgcttc tttcatattc
     2041 caatattacg gactagttat agatctttta ctattgggtc aggaaagggc tacagatttg
     2101 gctggtccag ctaacaatcc aaatgaattt atgcaattca agagcaaaga agtagaaaag
     2161 gcacatccga tcagacttta caccagatat ttagatcgta tatatatgct ttttcacttt
     2221 gaagaagatg agggagagga attgactgat gaatacttgg cagagaatcc agatccaaac
     2281 tttgaaaaca gcatcggata caataataga aaatgctggc ccaaagattc gcgcatgagg
     2341 ctaatacgtc aggacgttaa cctagggcga gcagtgtttt gggaaattca aagtagggtg
     2401 ccaacttcgt taacgtcaat taaatgggaa aatgcttttg tttccgtata cagtaaaaac
     2461 aatccgaact tactcttctc tatgtgtggt tttgaagtca gaatattacc aagacaaaga
     2521 atggaagagg tggtttctaa cgatgaaggt gtttgggatt tagttgacga aagaaccaag
     2581 caaagaactg caaaagcata tttaaaggta tctgaagaag aaatcaaaaa atttgacagc
     2641 agaataagag gcatattgat ggcctccggt tcaaccactt ttactaaagt ggccgctaaa
     2701 tggaacactt cactgatatc ccttttcaca tactttagag aagccattgt tgccacggag
     2761 ccgttattag atatcctggt gaaaggagaa acaagaattc aaaaccgtgt caaacttggt
     2821 ttaaattcga aaatgccaac aagatttcca cctgctgttt tttatacacc aaaagaactt
     2881 ggtgggttgg gtatgattag tgcttctcat attttaattc ctgcatctga cttaagttgg
     2941 tcaaaacaga ccgatactgg aattacgcat tttcgtgctg gtatgacgca cgaggatgaa
     3001 aaattaatcc caactatctt ccgttacatc actacttggg aaaatgaatt tttagactct
     3061 caaagggtgt gggccgagta tgcaacgaaa aggcaggaag ctattcagca gaataggagg
     3121 ttagcttttg aagaacttga aggatcgtgg gatcgtggta tacctcgtat tagcacttta
     3181 tttcaaagag atcgacatac tctagcgtat gacagaggtc acagaatccg tagggaattt
     3241 aaacagtact ctctagagag aaacagcccc ttttggtgga caaactccca ccatgacggt
     3301 aaattatgga atttaaatgc ctatagaact gatgtgattc aagccctagg aggtattgaa
     3361 acaattttgg aacacacttt atttaagggg actggtttta attcatggga aggtttgttt
     3421 tgggaaaagg cgtccgggtt tgaggattcc atgcaattta agaagctaac tcatgctcaa
     3481 agaactggtt taagccaaat tccaaatcgt agatttacct tatggtggtc accaactatt
     3541 aacagagcaa atgtctatgt cggtttcttg gtgcaattgg atctgactgg tatcttcctc
     3601 catggtaaaa ttcctacgtt aaaaatatct ttaattcaaa tattccgtgc tcatttgtgg
     3661 caaaaaatac atgaaagtat agtttttgat atttgtcaaa ttctcgatgg tgagctagat
     3721 gttttgcaaa ttgaatctgt gactaaagaa acagtgcatc cccgcaaatc gtacaagatg
     3781 aattcatctg ccgctgacat aactatggaa agtgttcatg aatgggaagt ttcaaaaccc
     3841 tccctattac atgaaacaaa tgactcattt aagggattga ttaccaacaa aatgtggttt
     3901 gatgtacaat tgagatacgg tgattatgat tctcatgata tatctcgtta tgtgagggca
     3961 aaattcctcg attatacaac agataatgtc agcatgtatc cctccccaac aggtgttatg
     4021 atcggtatcg atttggctta taacatgtat gatgcctatg gtaattggtt caacggatta
     4081 aaacctttga tacaaaatag tatgagaaca attatgaaag ctaacccagc attatatgtc
     4141 cttcgtgaac gtattagaaa aggtcttcaa atatatcaat ctagtgtaca ggaaccattt
     4201 ttaaactcct caaactacgc cgagttattc aataacgaca ttaaactctt cgttgacgac
     4261 actaatgtgt atagagttac tgtccacaag acttttgaag ggaacgttgc tacaaaagca
     4321 attaatggtt gcattttcac tttaaatcca aagactgggc atctgtttct gaaaattatc
     4381 catacctctg tatgggccgg tcaaaaacgt ttaagccaat tggccaaatg gaaaacagct
     4441 gaggaagtta gtgctcttgt gagatctttg cccaaagaag agcagccaaa acaaattatc
     4501 gtcacaagaa aggcaatgct tgatccttta gaggttcaca tgcttgactt tcctaacata
     4561 gcaatcaggc caacagagct gcgactacca ttttcagctg caatgtcaat agataaactt
     4621 tctgatgttg tgatgaaagc tactgaacct cagatggttc tgtttaacat ttatgatgat
     4681 tggttagacc gtatttcatc gtacactgca ttttctagac ttacactact tttaagagct
     4741 ttaaagacga acgaggaaag tgccaaaatg attctactca gtgaccccac gatcactatc
     4801 aaatcgtacc atttatggcc ttcattcact gatgaacaat ggatcactat cgaatctcaa
     4861 atgagagatt tgattttgac tgagtacggc agaaaataca atgtcaatat atctgcctta
     4921 actcaaacgg aaattaaaga tattatattg ggtcaaaata tcaaggcacc atctgtgaag
     4981 agacaaaaaa tggctgaatt agaagctgcc aggtcagaaa agcagaacga cgaagaagct
     5041 gcaggtgcat caactgttat gaagactaaa actatcaatg cgcaaggtga ggagatagtt
     5101 gtagtagctt ccgctgatta cgaaagtcaa acgtttagtt caaagaatga atggagaaaa
     5161 tctgcaattg ctaatacact attatatttg aggttaaaaa acatttatgt ttcggctgat
     5221 gatttcgtgg aggaacagaa tgtttacgtc cttccaaaaa atttattaaa gaagtttatt
     5281 gagatatccg atgtgaaaat ccaggtggcg gcctttattt atggtatgtc agcaaaagat
     5341 catccgaagg tgaaagaaat caagactgtt gtgttggtac ctcaattagg ccacgtagga
     5401 tccgttcaga taagcaacat ccctgacatc ggagaccttc cggatactga aggccttgaa
     5461 ctactcggtt ggatccatac ccagacagaa gaacttaaat tcatggctgc gtctgaagta
     5521 gcaactcatt ctaaactttt tgcggataag aaaagagact gcattgatat ttcaattttt
     5581 tccaccccgg gatcagtatc attaagcgct tataacctga ccgacgaagg ttaccaatgg
     5641 ggtgaagaaa ataaggatat aatgaacgtg ctatcggaag ggtttgagcc tactttcagc
     5701 acgcatgccc aattacttct ctctgaccgt atcacagata attttataat tccatctgga
     5761 aacgtttgga actacacatt tatgggaaca gcatttaacc aagaaggtga ctacaatttt
     5821 aaatacggca ttcctttgga attctataat gagatgcatc gtccagtaca ctttttacag
     5881 tttagcgaat tggcggggga cgaagagtta gaggccgaac aaatcgatgt atttagctga
     5941 atatattccc caataaactg gaatctgtta tttcatagat atatatgtaa gtataagttt
     6001 gtatgtataa tattcaacgc attttttatg cccgtcgtag ttgctaccta ccacacgatg
     6061 atgtattata atttcggaac tacttcaaag ctacgctttc cgcactatga aaagctataa
     6121 gtatgtgaaa attggtcatt gactgtgatt ctatttcaaa aagtaaccaa aggagataga
     6181 catcgttcgg acagaaaatg gtccgttgtc actcccattc cattatgtcc ttcatataga
     6241 aattggatat tcttgttttc atttcgcgca ataaagcaat tccgtgcggc agaaagaact
     6301 tatatatatt taactgatct tacgctattt atggcaaaac ttgtgttaca tttttgaaga
     6361 taaagttaca atcattacgg cagcctcaaa acaaaattgg gagaaaacat actcaagtga
     6421 gtactcattt tgtgcaagca aacactgaca attgaagaga tccacaggac gggtgtggtc
     6481 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
     6541 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat
     6601 agcgctagag cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta
     6661 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     6721 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     6781 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     6841 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6901 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6961 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7021 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7081 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7141 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7201 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7261 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7321 cacaccgcat agatccgtcg agttcaagag aaaaaaaaag aaaaagcaaa aagaaaaaag
     7381 gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg tcatcttcag
     7441 tatcatactg ttcgtataca tacttactga cattcatagg tatacatata tacacatgta
     7501 tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac acctttggtg
     7561 gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac cgcaagagcc
     7621 ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc aacgtggagg
     7681 gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc gcaagagaga
     7741 tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct gttcgaaaga
     7801 tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc aaaccttttt
     7861 actccacgca cggcccctag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
     7921 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
     7981 ccggaatgct tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg
     8041 ttaatcactt gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag
     8101 atcgagtgct ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt
     8161 tcatttgtaa tacgctttac tagggctttc tgctctgtca tctttgcctt cgtttatctt
     8221 gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat
     8281 tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa
     8341 aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga ggaggccaag
     8401 agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg actaatgccg
     8461 tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa acgggaattt
     8521 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     8581 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     8641 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     8701 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     8761 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8821 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8881 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8941 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9001 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9061 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9121 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9181 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9241 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9301 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9361 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9421 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9481 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9541 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9601 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9661 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9721 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     9961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10561 cttttgctgg ccttttgctc acatgt
//
