LOCUS       sequence-217128-        9042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent, functional, glutamate receptor subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE
  TITLE     Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid
            receptor subunits that are permissive for the insertion of green 
            fluorescent protein.
  JOURNAL   Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12.
  PUBMED    16765522
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience."; date: "2006-08-25"; volume: "141(2)"; pages: 
            "837-49. Epub 2006 Jun 12"
COMMENT     Sequence Label: pR2Q024y
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      15..603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     504..523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     757..774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    891..912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(927..932,933..950,951..957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    965..981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     970..992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     989..1005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     989..1005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1034..1413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1414..1617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1568..1588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1614..1638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1848..1866
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1848..1865
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1879..1898
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1956..1972
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1956..1972
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    2861..2879
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             2888..3598
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(2927..2948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3188..3207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3535..3556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3616..3634
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     regulatory      6362..6371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6375..6509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6412..6431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6466..6485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6578..6935
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6578..6769
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6601..6621)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6786..6921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6955..6972)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6955..6971)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6964..6986)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7184..7639
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7271..7290)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7481..7502
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7656..7675)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7775..7797
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7835..7853)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7921..8025
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8026..8094,8095..8886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8244..8263)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
       61 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      901 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      961 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattaa
     1021 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
     1081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     1141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     1441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     1561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     1621 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     1681 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
     1741 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
     1801 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     1861 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
     1921 acctcggttc tatcgattga attccccggg gatcctctag aactagtgga tcccccgggc
     1981 tgcaggaatt cggcacgagg ccagtcctcg ggacttcggg agcagggaca ggacgcaagg
     2041 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt
     2101 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa
     2161 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact
     2221 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg
     2281 actgattttt ggtgtctctt ctaacagcat acagataggg gggctatttc caaggggcgc
     2341 tgatcaagaa tacagtgcat ttcgggtagg gatggttcag ttttccactt cggagttcag
     2401 actgacaccc catatcgaca atttggaggt agccaacagt ttcgcagtca ccaatgcttt
     2461 ctgctcccag ttttcaagag gagtctacgc aatttttgga ttttatgaca agaagtctgt
     2521 aaataccatc acatcattct gtgggacact ccatgtgtcc ttcatcacac ctagcttccc
     2581 aacagatggc acacatccat ttgtcatcca gatgcgacct gacctcaaag gagcactcct
     2641 tagcttgatt gagtactacc aatgggacaa gttcgcatac ctctatgaca gtgacagagg
     2701 cttatcaaca ctgcaagctg ttctggattc tgctgcagag aagaagtggc aggtgactgc
     2761 tatcaatgtg gggaacatca acaatgacaa gaaagatgag acctacagat cgctctttca
     2821 agatctggag ttaaaaaaag aacggcgtgt aatcctggac ctgtctctta tacacatctg
     2881 gcgcgccagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2941 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3001 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3061 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     3121 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3181 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3241 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3301 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3361 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3421 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3481 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3541 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     3601 tgggcgcgcc cgggcagatg tgtataagag acagatcctg gactgtgaaa gggataaagt
     3661 aaatgacatt gtggaccagg ttattaccat tggaaaacat gttaaagggt accattatat
     3721 cattgcaaat ctgggattca ctgatgggga cctgctgaaa attcagtttg gaggagcaaa
     3781 tgtctctgga tttcagattg tagactacga tgattccctg gtgtctaaat ttatagaaag
     3841 atggtcaaca ctggaagaga aagaataccc tggagcacac acagcgacaa ttaagtatac
     3901 ttcggccctg acgtatgatg ctgtccaagt gatgactgaa gcattccgta accttcggaa
     3961 gcagaggatt gaaatatccc ggagaggaaa tgcaggggat tgtttggcca acccagctgt
     4021 gccctgggga caaggggtcg aaatagaaag ggccctcaag caggttcaag ttgaaggcct
     4081 ctctggaaat ataaagtttg accagaatgg aaaacgaata aactacacaa ttaacatcat
     4141 ggagctcaaa acaaatggac cccggaagat tgggtactgg agtgaagtgg ataaaatggt
     4201 tgtcacccta actgagctcc catcaggaaa tgacacgtct gggcttgaaa acaagactgt
     4261 ggtggtcacc acaatattgg aatctccata tgttatgatg aagaaaaatc atgaaatgct
     4321 tgaagggaat gagcgttacg agggctactg tgttgactta gctgcagaaa ttgccaaaca
     4381 ctgtgggttc aagtacaagc tgactattgt tggggatggc aagtatgggg ccagggatgc
     4441 cgacaccaaa atttggaatg gtatggttgg agagcttgtc tacgggaaag ctgacattgc
     4501 aattgctcca ttaactatca ctctcgtgag agaagaggtg attgacttct ccaagccctt
     4561 catgagtctt ggaatctcta tcatgatcaa gaagcctcag aagtccaaac caggagtgtt
     4621 ttcctttctt gatcctttag cctatgagat ctggatgtgc attgtgtttg cctacattgg
     4681 ggtcagtgta gttttattcc tggtcagcag atttagcccc tacgagtggc acactgagga
     4741 atttgaagat ggaagagaaa cacaaagtag tgaatcaact aatgaatttg ggatttttaa
     4801 tagtctctgg ttttccttgg gtgcctttat gcagcaggga tgcgatattt cgccaagatc
     4861 cctctctggg cgcattgttg gaggtgtgtg gtggttcttt accctgatca taatctcctc
     4921 ctacacggct aacttagctg ccttcctgac tgtagagagg atggtgtctc ccatcgaaag
     4981 tgctgaggat ctgtctaagc aaacagaaat tgcttatgga acattagact ctggctccac
     5041 taaagagttt ttcaggagat ctaaaatcgc agtgtttgat aaaatgtgga cttatatgag
     5101 gagtgcagag ccctctgtgt ttgtgaggac taccgcagaa ggagtagcca gagtccggaa
     5161 atccaaagga aagtatgcct acttgctgga gtccacaatg aacgagtaca tcgagcagag
     5221 gaagccttgt gacaccatga aagtgggagg aaacttggat tccaaaggct acggcatcgc
     5281 cacacctaaa ggatcctcat taggaacccc agtaaatctt gcagtattga aactcagtga
     5341 gcaaggcgtc ttagacaagc tgaaaaacaa atggtggtac gataaaggtg aatgtggagc
     5401 caaggactcg ggaagtaagg aaaagaccag tgccctcagt ctgagcaacg ttgctggagt
     5461 attctacatc cttgtcgggg gccttggttt ggcaatgctg gtggctttga ttgagttctg
     5521 ttacaagtca agggccgagg cgaaacgaat gaaggtggca aagaatccac agaatattaa
     5581 cccatcttcc tcgcagaatt cccagaattt tgcaacttat aaggaaggtt acaacgtata
     5641 tggcatcgag agtgttaaaa tttaggggat gaccttgagt gatgtcatga ggagcaaggc
     5701 aaggctgtca attacaggaa gtactggaga aaatggacgt gttatgactc cagaatttcc
     5761 caaagcagtg catgctgtcc cttacgtgag tcctggcatg ggaatgaatg tcagtgtgac
     5821 tgatctctcg tgattgatag gaaccttctg agtgccttac acaatggttt ccttgtgtgt
     5881 ttattgtcaa agtggtgaga ggcatccgat atcttgaagg cttttctttc agccaagaat
     5941 tcttaactat gtggagttca ccttgaattg taaggaaaga taaattacaa acagagcatc
     6001 attttctact ccgatatcag aggaagcgtg gtggacatgc acagctaaca tggaaatact
     6061 atcatttaac tgaagtcttt gtacagacaa caaacccgtt tccgcagcca ctattgttag
     6121 tctcttgatt cataatgact taagcacact tgacatcaac tgcatcaaga tgtgacctgt
     6181 tttataaata aaaaggaaaa aaacatttaa aataaaaaaa tatttttagg tattttcaca
     6241 gacaaactgg cttttaaata aatttgcttc cgtactggtt ggttaagaca aatacaaaaa
     6301 caaaaacaaa acaaaacaaa aaccaaaaaa aaaaaaaaaa aactcgacct gcagaagctt
     6361 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6421 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6481 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     6541 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     6601 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6661 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     6961 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7021 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7081 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     7141 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     7201 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7261 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7321 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7381 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7441 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7501 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     7561 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7621 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7681 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7741 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7801 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7861 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7921 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7981 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8041 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8101 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8161 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8221 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8281 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8341 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8401 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8461 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8521 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8581 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8641 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8701 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8761 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8821 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8881 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8941 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9001 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa ag
//
