LOCUS       sequence-21715-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Fan L, Freeman KW, Khan T, Pham E, Spencer DM
  TITLE     Improved artificial death switches based on caspases and FADD.
  JOURNAL   Hum Gene Ther. 1999 Sep 20. 10(14):2273-85.
  PUBMED    10515447
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 1999 Sep 20. 10(14):2273-85."
COMMENT     Sequence Label: pSH1/M-Fv'-Fvls-E
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..58)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(83..403)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tccggtcatc aatcaggaat tcttagtcga gtgcgtagtc tggtacgtcg tacggatagt
       61 cgactccgga tccaccgcca gattccagtt ttagaagctc cacatcgaag acgagagtgg
      121 catgtggtgg gatgatgcct gggtgcccag tggcaccata ggcataatct ggagatatag
      181 tcagtttggc tctctgaccc acactcatct gggcaacccc ttcttcccag cctcggatca
      241 cctcctgctt gcctagcata aacttaaagg gcttgtttct gtcccgggag gaatcaactt
      301 tctttccatc ttcaagcatc ccggtgtagt gcaccacgca ggtctggccg cgcttgggga
      361 aggtgcgccc gtctcctggg gagatagtct ccacctgcac tccctcgact tcgagcttca
      421 acagttcgac gtcaaacacc aaggtagcgt gagggggaat aattccggga tgtccggtag
      481 cgccgtatgc gtagtcgggg ctgatggtga gcttagcgcg ttggccgacg gacatttgag
      541 ccacgccctc ctcccatccc ctaatgactt cttgttttcc caacatgaat ttgaaaggtt
      601 tattgcgatc tctactactg tccacctttt tgccgtcctc caacatgcct gtataatgga
      661 cgacacatgt ttgtcccctt ttaggaaatg ttctgccatc g
//
