LOCUS       sequence-217159-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRS314-SNU114.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314-SNU114
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     82..99
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    216..237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(252..257,258..275,276..282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    290..306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     295..317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     314..330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     314..330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        351..369
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3961..3980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3962..3980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3987..4004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3987..4003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3996..4018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4144..4599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4231..4250)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4441..4462
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4702..5376)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5377..5657)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5678..5697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5797..5819
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5857..5875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5916..6419
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6456..6560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6561..6629,6630..7421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6779..6798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7592..8180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8081..8100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
       61 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
      121 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
      181 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      241 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
      301 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct
      361 cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtagcgtat actctggcat
      421 ttttcattta tttgacttat ttttatttat tttattgtac aatggcaaac aaatacacgg
      481 actggtatgt tcttcgtcaa attgatgatc tgttcttttg gcttatcctt atgggatgat
      541 taatgattgt taaagcctca ttgcggaaat tttaatgccg gaaaggcctg aaaaatttca
      601 aagtggcaga aaacaaggct ttggggcatt acttgccaac ggctgacgat tgctagctgc
      661 ttttacagaa gcgatacaca atagctccta acatagcaaa atggaaggtg acgatttatt
      721 cgatgagttt ggaaatttga tcggagttga tccttttgac tcggatgaag aagaaagtgt
      781 gctggatgag caagaacaat atcaaaccaa tacgtttgag gggagcggca acaacaatga
      841 gattgagagc agacaactta cctcgttagg aagtaaaaag gaacttggaa tttccttaga
      901 gcacccttat ggtaaggaag tggaagtatt aatggaaact aagaacacac agtcaccaca
      961 gactccactg gtagagccgg ttacagaacg aacaaagttg caagagcata caatttttac
     1021 acaattaaaa aaaaacattc cgaagactag atataatcga gactatatgt tatcaatggc
     1081 taacattccg gagaggataa taaatgttgg tgtcattgga cctcttcact caggtaagac
     1141 ctctttgatg gatctcctag taatcgactc acacaaacgc ataccagata tgtctaagaa
     1201 tgtagaactt ggatggaaac cgctaagata cctggataat ttgaaacaag agattgaccg
     1261 gggtctttcc attaaactta atggttccac tctactctgc accgatttag aatccaagtc
     1321 tagaatgata aactttttgg atgcaccagg gcatgttaat tttatggatg aaacggcagt
     1381 cgcacttgca gcaagtgatc tggttttaat cgtgatagat gttgtagaag gtgttacatt
     1441 tgtggtagag caattgataa aacaaagtat taaaaataat gtagcaatgt gttttgttat
     1501 aaataaactc gacagattga ttctagattt aaagttgcca ccaatggacg catacttgaa
     1561 gttaaatcat ataattgcaa atatcaattc ctttacgaag ggaaacgtgt tttcaccaat
     1621 agacaataac ataatatttg cgtctacaaa gctgggcttc acattcacta tcaaggaatt
     1681 tgtttcttat tattacgccc actccatacc ttcttctaag atagacgact ttaccacaag
     1741 gctatggggt agcgtatatt accataaagg taatttcagg acaaaaccat ttgaaaatgt
     1801 agagaagtac ccgactttcg ttgaatttat cctcatcccg ctttataaaa tattttccta
     1861 cgctttatct atggagaaag ataaattaaa gaatttgtta aggtctaact ttagagttaa
     1921 tttaagtcaa gaggccctcc agtacgatcc gcaacctttt ttaaaacacg ttctacagtt
     1981 aatatttagg caacaaaccg gtcttgtaga tgcgataacc agatgttatc aaccatttga
     2041 attattcgac aataagacag ctcatctttc aattccagga aaatctacac cagaaggcac
     2101 tctttgggct catgtactga aaactgtgga ttacggtgga gcagagtggt cccttgtacg
     2161 gatatactct ggacttttga agagagggga tacagtacgt atacttgata catcacaatc
     2221 tgaatctcgc caaaaaagac agttgcatga tatctcaaag acggaaacct ccaacgaaga
     2281 tgaagatgaa gatgacgaaa cgccttcatg tgaggtcgaa gaaattggct tattaggtgg
     2341 cagatatgtc tatccggttc atgaagcaca caaaggacag atagtattaa taaaaggtat
     2401 ttcaagtgca tatattaaat ccgcgacgtt gtactctgta aagagtaagg aggacatgaa
     2461 acagctaaaa tttttcaaac ctttagatta tattaccgag gcagttttca aaattgttct
     2521 tcaaccttta ctgccaaggg aattacccaa gttattggat gccctgaaca aaatttcgaa
     2581 atactaccca ggcgttatta ttaaagttga ggaatctgga gaacatgtaa tactaggaaa
     2641 tggagagctg tatatggact gtttgttgta tgatctaaga gcgagttatg caaagataga
     2701 aattaaaata tctgatcctt taactgtttt ttctgaaagt tgctcaaatg aatcgttcgc
     2761 ttcaatacct gtaagtaatt cgatatctcg ccttggtgag gaaaatttac caggtctgtc
     2821 gattagtgta gccgctgaac caatggactc taaaatgatt caggatttga gcaggaacac
     2881 attgggaaag ggtcaaaact gcttggatat tgacggaata atggacaacc caaggaaact
     2941 atccaaaata ttgagaaccg agtatgggtg ggattcgttg gcatcaagaa acgtttggtc
     3001 tttttataat ggcaacgtgt tgattaatga tactttgcca gatgaaatca gtcctgaatt
     3061 attatccaaa tataaggaac aaataataca aggattttac tgggctgtaa aagaggggcc
     3121 tttggcagaa gaaccaattt atggtgtaca gtataaattg ttatcgatct cagtaccttc
     3181 cgacgtaaac attgacgtta tgaaaagtca aattattccg ctaatgaaaa aagcctgtta
     3241 cgttggctta ctgacagcaa tcccaatttt attggaaccc atctatgaag ttgacatcac
     3301 ggtccacgcc cccttgctgc caatagtaga ggaacttatg aagaagagac gtggaagcag
     3361 gatatacaaa acaataaaag tggcagggac accattgttg gaggttcgtg gacaagttcc
     3421 ggttattgaa tctgcaggat tcgagacaga tttgagatta tctacgaatg gtcttggtat
     3481 gtgtcagctg tacttttggc acaagatatg gaggaaggtt cctggtgatg ttttggataa
     3541 agatgcgttt attccaaaat tgaaacccgc acctatcaac agtttaagtc gtgatttcgt
     3601 gatgaaaaca agaaggcgga agggtatttc tacaggtgga tttatgtcaa atgatggtcc
     3661 tacgcttgaa aagtatataa gcgctgaatt atacgctcaa ttaagagaaa atggcttagt
     3721 accgtgaaaa tcttggcgca taagaattaa gcaatatgtc cacaatattt ttattcagca
     3781 gaatttacat atgacttcgt atttagtgat ataactactc atcgggactg aaaatttttt
     3841 atttctgcct aaatacagat gagatgagct ttatagaatt ttacgctgtt aaaaagttaa
     3901 acggtaacaa agcacagtta gttgaacaac ggtatataga gaatggcgag ctccaattcg
     3961 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4141 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4201 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4261 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4321 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4381 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4441 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4501 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4561 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
     4621 tacgcatctg tgcggtattt cacaccgcat agatcggcaa gtgcacaaac aatacttaaa
     4681 taaatactac tcagtaataa cctatttctt agcatttttg acgaaatttg ctattttgtt
     4741 agagtctttt acaccatttg tctccacacc tccgcttaca tcaacaccaa taacgccatt
     4801 taatctaagc gcatcaccaa cattttctgg cgtcagtcca ccagctaaca taaaatgtaa
     4861 gctttcgggg ctctcttgcc ttccaaccca gtcagaaatc gagttccaat ccaaaagttc
     4921 acctgtccca cctgcttctg aatcaaacaa gggaataaac gaatgaggtt tctgtgaagc
     4981 tgcactgagt agtatgttgc agtcttttgg aaatacgagt cttttaataa ctggcaaacc
     5041 gaggaactct tggtattctt gccacgactc atctccatgc agttggacga tatcaatgcc
     5101 gtaatcattg accagagcca aaacatcctc cttaggttga ttacgaaaca cgccaaccaa
     5161 gtatttcgga gtgcctgaac tatttttata tgcttttaca agacttgaaa ttttccttgc
     5221 aataaccggg tcaattgttc tctttctatt gggcacacat ataataccca gcaagtcagc
     5281 atcggaatct agagcacatt ctgcggcctc tgtgctctgc aagccgcaaa ctttcaccaa
     5341 tggaccagaa ctacctgtga aattaataac agacatactc caagctgcct ttgtgtgctt
     5401 aatcacgtat actcacgtgc tcaatagtca ccaatgccct ccctcttggc cctctccttt
     5461 tcttttttcg accgaattaa ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt
     5521 gtacgtaagg tgacaagcta tttttcaata aagaatatct tccactactg ccatctggcg
     5581 tcataactgc aaagtacaca tatattacga tgctgttcta ttaaatgctt cctatattat
     5641 atatatagta atgtcgtgat ctatggtgca ctctcagtac aatctgctct gatgccgcat
     5701 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5761 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     5821 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     5881 aggttaatgt catgataata atggtttctt agacggatcg cttgcctgta acttacacgc
     5941 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta
     6001 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga
     6061 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt
     6121 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa
     6181 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa
     6241 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc
     6301 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt
     6361 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg
     6421 aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6781 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8221 gctggccttt tgctcacatg ttctttcctg cg
//
