LOCUS       sequence-21716-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Fan L, Freeman KW, Khan T, Pham E, Spencer DM
  TITLE     Improved artificial death switches based on caspases and FADD.
  JOURNAL   Hum Gene Ther. 1999 Sep 20. 10(14):2273-85.
  PUBMED    10515447
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 1999 Sep 20. 10(14):2273-85."
COMMENT     Sequence Label: pSH1/Sn-E-Fv'Fvls-E
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..68)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(93..413)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 gntnngnngg atcggtcatc aatcaggaat tcttagtcga gtgcgtagtc tggtacgtcg
       61 tacggatagt cgactccgga tccaccgcca gattccagtt ttagaagctc cacatcgaag
      121 acgagagtgg catgtggtgg gatgatgcct gggtgcccag tggcaccata ggcataatct
      181 ggagatatag tcagtttggc tctctgaccc acactcatct gggcaacccc ttcttcccag
      241 cctcggatca cctcctgctt gcctagcata aacttaaagg gcttgtttct gtcccgggag
      301 gaatcaactt tctttccatc ttcaagcatc ccggtgtagt gcaccacgca ggtctggccg
      361 cgcttgggga aggtgcgccc gtctcctggg gagatagtct ccacctgcac tccctcgact
      421 tcgagcttca acagttcgac gtcaaacacc aaggtagcgt gagggggaat aattccggga
      481 tgtccggtag cgccgtatgc gtagtcgggg ctgatggtga gcttagcgcg ttggccgacg
      541 gacatttgag ccacgccctc ctcccatccc ctaatgactt cttgttttcc caacatgaat
      601 ttgaaaggtt tattgcgatc tctactanct ncnnnttttt tnncgcctca nnaaangcgc
      661 ctgtataatg aacgacacat gtttgtcccc ttttaggaaa tgttctgcca tcgccgggac
      721 taatggttnc nattgaaacc ccctcgagtg cgtagtctgg aantcgtacg anatcnatcc
      781 atggngccgn gccgcttanc nnnatgaggt aaaacctttn annaaaaanc tncnangnct
      841 aaaaangcan ncnngggaat tttttt
//
