LOCUS       sequence-217161-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  snu114-14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114-14
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    90..111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(126..131,132..149,150..156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     169..191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     188..204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     188..204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        225..243
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3835..3854)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3836..3854)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3861..3878)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3861..3877)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3870..3892)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4018..4473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4105..4124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4315..4336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4576..5250)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5251..5531)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5552..5571)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5671..5693
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5731..5749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5790..6293
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6330..6434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6435..6503,6504..7295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6653..6672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7466..8054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7955..7974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8208..8225
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
       61 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      121 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      181 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
      241 agggaacaaa agctggtacc gggccccccc tcgaggtagc gtatactctg gcatttttca
      301 tttatttgac ttatttttat ttattttatt gtacaatggc aaacaaatac acggactggt
      361 atgttcttcg tcaaattgat gatctgttct tttggcttat ccttatggga tgattaatga
      421 ttgttaaagc ctcattgcgg aaattttaat gccggaaagg cctgaaaaat ttcaaagtgg
      481 cagaaaacaa ggctttgggg cattacttgc caacggctga cgattgctag ctgcttttac
      541 agaagcgata cacaatagct cctaacatag caaaatggaa ggtgacgatt tattcgatga
      601 gtttggaaat ttgatcggag ttgatccttt tgactcggat gaagaagaaa gtgtgctgga
      661 tgagcaagaa caatatcaaa ccaatacgtt tgaggggagc ggcaacaaca atgagattga
      721 gagcagacaa cttacctcgt taggaagtaa aaaggaactt ggaatttcct tagagcaccc
      781 ttatggtaag gaagtggaag tattaatgga aactaagaac acacagtcac cacagactcc
      841 actggtagag ccggttacag aacgaacaaa gttgcaagag catacaattt ttacacaatt
      901 aaaaaaaaac attccgaaga ctagatataa tcgagactat atgttatcaa tggctaacat
      961 tccggagagg ataataaatg ttggtgtcat tggacctctt cactcaggta agacctcttt
     1021 gatggatctc ctagtaatcg actcacacaa acgcatacca gatatgtcta agaatgtaga
     1081 acttggatgg aaaccgctaa gatacctgga taatttgaaa caagagattg accggggtct
     1141 ttccattaaa cttaatggtt ccactctact ctgcaccgat ttagaatcca agtctagaat
     1201 gataaacttt ttggatgcac cagggcatgt taattttatg gatgaaacgg cagtcgcact
     1261 tgcagcaagt gatctggttt taatcgtgat agatgttgta gaaggtgtta catttgtggt
     1321 agagcaattg ataaaacaaa gtattaaaaa taatgtagca atgtgttttg ttataaataa
     1381 actcgacaga ttgattctag atttaaagtt gccaccaatg gacgcatact tgaagttaaa
     1441 tcatataatt gcaaatatca attcctttac gaagggaaac gtgttttcac caatagacaa
     1501 taacataata tttgcgtcta caaagctggg cttcacattc actatcaagg aatttgtttc
     1561 ttattattac gcccactcca taccttcttc taagatagac gactttacca caaggctatg
     1621 gggtagcgta tattaccata aaggtaattt caggacaaaa ccatttgaaa atgtagagaa
     1681 gtacccgact ttcgttgaat ttatcctcat cccgccttat aaaatatttt cctacgcttt
     1741 atctatggag aaagataaat taaagaattt gttaaggtct aactttagag ttaatttaag
     1801 tcaagaggcc ctccagtacg atccgcaacc ttttttaaaa cacgttctac agttaatatt
     1861 taggcaacaa accggtcttg tagatgcgat aaccagatgt tatcaaccat ttgaattatt
     1921 cgacaataag acagctcatc tttcaattcc aggaaaatct acaccagaag gcactctttg
     1981 ggctcatgta ctgaaaactg tggattacgg tggagcagag tggtcccttg tacggatata
     2041 ctctggactt ttgaagagag gggatacagt acgtatactt gatacatcac aatctgaatc
     2101 tcgccaaaaa agacagttgc atgatatctc aaagacggaa acctccaacg aagatgaaga
     2161 tgaagatgac gaaacgcctt catgtgaggt cgaagaaatt ggcttattag gtggcagata
     2221 tgtctatccg gttcatgaag cacacaaagg acagatagta ttaataaaag gtatttcaag
     2281 tgcatatatt aaatccgcga cgttgtactc tgtaaagagt aaggaggaca tgaaacagct
     2341 aaaatttttc aaacctttag attatattac cgaggcagtt ttcaaaattg ttcttcaacc
     2401 tttactgcca agggaattac ccaagttatt ggatgccctg aacaaaattt cgaaatacta
     2461 cccaggcgtt attattaaag ttgaggaatc tggagaacat gtaatactag gaaatggaga
     2521 gctgtatatg gactgtttgt tgtatgatct aagagcgagt tatgcaaaga tagaaattaa
     2581 aatatctgat cctttaactg ttttttctga aagttgctca aatgaatcgt tcgcttcaat
     2641 acctgtaagt aattcgatat ctcgccttgg tgaggaaaat ttaccaggtc tgtcgattag
     2701 tgtagccgct gaaccaatgg actctaaaat gattcaggat ttgagcagga acacattggg
     2761 aaagggtcaa aactgcttgg atattgacgg aataatggac aacccaagga aactatccaa
     2821 aatattgaga accgagtatg ggtgggattc gttggcatca agaaacgttt ggtcttttta
     2881 taatggcaac gtgttgatta atgacacttt gccagatgaa atcagtcctg aattattatc
     2941 caaatataag gaacaaataa tacaaggatt ttactgggct gtaaaagagg ggcctttggc
     3001 agaagaacca atttatggtg tacagtataa attgttatcg atctcagtac cttccgacgt
     3061 aaacattgac gttatgaaaa gtcaaattat tccgctaatg aaaaaagcct gttacgttgg
     3121 cttactgaca gcaatcccaa ttttattgga acccatctat gaagttgaca tcacggtcca
     3181 cgcccccttg ctgccaatag tagaggaact tatgaagaag agacgtggaa gcaggatata
     3241 caaaacaata aaagtggcag ggacaccatt gttggaggtt cgtggacaag ttccggttat
     3301 tgaatctgca ggattcgaga cagatttgag attatctacg aatggtcttg gtatgtgtca
     3361 gctgtacttt tggcacaaga tatggaggaa ggttcctggt gatgttttgg ataaagatgc
     3421 gtttattcca aaattgaaac ccgcacctat caacagttta agtcgtgatt tcgtgatgaa
     3481 aacaagaagg cggaagggta tttctacagg tggatttatg tcaaatgatg gtcctacgct
     3541 tgaaaagtat ataagcgctg aattatacgc tcaattaaga gaaaatggct tagtaccgtg
     3601 aaaatcttgg cgcataagaa ttaagcaata tgtccacaat atttttattc agcagaattt
     3661 acatatgact tcgtatttag tgatataact actcatcggg actgaaaatt ttttatttct
     3721 gcctaaatac agatgagatg agctttatag aattttacgc tgttaaaaag ttaaacggta
     3781 acaaagcaca gttagttgaa caacggtata tagagaatgg cgagctccaa ttcgccctat
     3841 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3901 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3961 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     4021 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4081 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4141 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4201 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4261 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4321 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4441 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     4501 tctgtgcggt atttcacacc gcatagatcg gcaagtgcac aaacaatact taaataaata
     4561 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc
     4621 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct
     4681 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctttc
     4741 ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa gttcacctgt
     4801 cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg aagctgcact
     4861 gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca aaccgaggaa
     4921 ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa tgccgtaatc
     4981 attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa ccaagtattt
     5041 cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc ttgcaataac
     5101 cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt cagcatcgga
     5161 atctagagca cattctgcgg cctctgtgct ctgcaagccg caaactttca ccaatggacc
     5221 agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt gcttaatcac
     5281 gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt
     5341 ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc tccggatcaa gattgtacgt
     5401 aaggtgacaa gctatttttc aataaagaat atcttccact actgccatct ggcgtcataa
     5461 ctgcaaagta cacatatatt acgatgctgt tctattaaat gcttcctata ttatatatat
     5521 agtaatgtcg tgatctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5581 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5641 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5701 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5761 atgtcatgat aataatggtt tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg
     5821 tatcttttaa tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt
     5881 gtatttggat tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa
     5941 aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga
     6001 caagaaaagc agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac
     6061 aggattttcg tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg
     6121 agagcaggaa gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac
     6181 atcttcggaa aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat
     6241 ttatatattt atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg
     6301 acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6361 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6421 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6481 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6541 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6601 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6661 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6721 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6781 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6841 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6901 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6961 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7021 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7081 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7141 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7201 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7261 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8161 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8221 gcgaggaagc ggaagagcgc ccaatacgca aa
//
