LOCUS       sequence-217162-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRS314 snu114-15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Brenner TJ, Guthrie C
  TITLE     Genetic analysis reveals a role for the C terminus of the 
            Saccharomyces cerevisiae GTPase Snu114 during spliceosome 
            activation.
  JOURNAL   Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044.
            Epub 2005 May 23.
  PUBMED    15911574
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.042044"; journalName: "Genetics"; date: 
            "2005-07"; volume: "170"; issue: "3"; pages: "1063-80"
COMMENT     Sequence Label: pRS314 snu114-15
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..58
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    175..196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(211..216,217..234,235..241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     254..276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     273..289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     273..289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        310..328
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3920..3939)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3921..3939)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3946..3963)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3946..3962)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3955..3977)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4103..4558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4190..4209)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4400..4421
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4661..5335)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(5336..5616)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(5637..5656)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5756..5778
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5816..5834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5875..6378
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6415..6519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6520..6588,6589..7380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6738..6757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7551..8139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8040..8059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
       61 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      241 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      301 caagctcgaa attaaccctc actaaaggga acaaaagctg gtaccgggcc ccccctcgag
      361 gtagcgtata ctctggcatt tttcatttat ttgacttatt tttatttatt ttattgtaca
      421 atggcaaaca aatacacgga ctggtatgtt cttcgtcaaa ttgatgatct gttcttttgg
      481 cttatcctta tgggatgatt aatgattgtt aaagcctcat tgcggaaatt ttaatgccgg
      541 aaaggcctga aaaatttcaa agtggcagaa aacaaggctt tggggcatta cttgccaacg
      601 gctgacgatt gctagctgct tttacagaag cgatacacaa tagctcctaa catagcaaaa
      661 tggaaggtga cgatttattc gatgagtttg gaaatttgat cggagttgat ccttttgact
      721 cggatgaaga agaaagtgtg ctggatgagc aagaacaata tcaaaccaat acgtttgagg
      781 ggagcggcaa caacaatgag attgagagca gacaacttac ctcgttagga agtaaaaagg
      841 aacttggaat ttccttagag cacccttatg gtaaggaagt ggaagtatta atggaaacta
      901 agaacacaca gtcaccacag actccactgg tagagccggt tacagaacga acaaagttgc
      961 aagagcatac aatttttaca caattaaaaa aaaacattcc gaagactaga tataatcgag
     1021 actatatgtt atcaatggct aacattccgg agaggataat aaatgttggt gtcattggac
     1081 ctcttcactc aggtatcacc tctttgatgg atctcctagt aatcgactca cacaaacgca
     1141 taccagatat gtctaagaat gtagaacttg gatggaaacc gctaagatac ctggataatt
     1201 tgaaacaaga gattgaccgg ggtctttcca ttaaacttaa tggttccact ctactctgca
     1261 ccgatttaga atccaagtct agaatgataa actttttgga tgcaccaggg catgttaatt
     1321 ttatggatga aacggcagtc gcacttgcag caagtgatct ggttttaatc gtgatagatg
     1381 ttgtagaagg tgttacattt gtggtagagc aattgataaa acaaagtatt aaaaataatg
     1441 tagcaatgtg ttttgttata aataaactcg acagattgat tctagattta aagttgccac
     1501 caatggacgc atacttgaag ttaaatcata taattgcaaa tatcaattcc tttacgaagg
     1561 gaaacgtgtt ttcaccaata gacaataaca taatatttgc gtctacaaag ctgggcttca
     1621 cattcactat caaggaattt gtttcttatt attacgccca ctccatacct tcttctaaga
     1681 tagacgactt taccacaagg ctatggggta gcgtatatta ccataaaggt aatttcagga
     1741 caaaaccatt tgaaaatgta gagaagtacc cgactttcgt tgaatttatc ctcatcccgc
     1801 tttataaaat attttcctac gctttatcta tggagaaaga taaattaaag aatttgttaa
     1861 ggtctaactt tagagttaat ttaagtcaag aggccctcca gtacgatccg caaccttttt
     1921 taaaacacgt tctacagtta atatttaggc aacaaaccgg tcttgtagat gcgataacca
     1981 gatgttatca accatttgaa ttattcgaca ataagacagc tcatctttca attccaggaa
     2041 aatctacacc agaaggcact ctttgggctc atgtactgaa aactgtggat tacggtggag
     2101 cagagtggtc ccttgtacgg atatactctg gacttttgaa gagaggggat acagtacgta
     2161 tacttgatac atcacaatct gaatctcgcc aaaaaagaca gttgcatgat atctcaaaga
     2221 cggaaacctc caacgaagat gaagatgaag atgacgaaac gccttcatgt gaggtcgaag
     2281 aaattggctt attaggtggc agatatgtct atccggttca tgaagcacac aaaggacaga
     2341 tagtattaat aaaaggtatt tcaagtgcat atattaaatc cgcgacgttg tactctgtaa
     2401 agagtaagga ggacatgaaa cagctaaaat ttttcaaacc tttagattat attaccgagg
     2461 cagttttcaa aattgttctt caacctttac tgccaaggga attacccaag ttattggatg
     2521 ccctgaacaa aatttcgaaa tactacccag gcgttattat taaagttgag gaatctggag
     2581 aacatgtaat actaggaaat ggagagctgt atatggactg tttgttgtat gatctaagag
     2641 cgagttatgc aaagatagaa attaaaatat ctgatccttt aactgttttt tctgaaagtt
     2701 gctcaaatga atcgttcgct tcaatacctg taagtaattc gatatctcgc cttggtgagg
     2761 aaaatttacc aggtctgtcg attagtgtag ccgctgaacc aatggactct aaaatgattc
     2821 aggatttgag caggaacaca ttgggaaagg gtcaaaactg cttggatatt gacggaataa
     2881 tggacaaccc aaggaaacta tccaaaatat tgagaaccga gtatgggtgg gattcgttgg
     2941 catcaagaaa cgtttggtct ttttataatg gcaacgtgtt gattaatgac actttgccag
     3001 atgaaatcag tcctgaatta ttatccaaat ataaggaaca aataatacaa ggattttact
     3061 gggctgtaaa agaggggcct ttggcagaag aaccaattta tggtgtacag tataaattgt
     3121 tatcgatctc agtaccttcc gacgtaaaca ttgacgttat gaaaagtcaa attattccgc
     3181 taatgaaaaa agcctgttac gttggcttac tgacagcaat cccaatttta ttggaaccca
     3241 tctatgaagt tgacatcacg gtccacgccc ccttgctgcc aatagtagag gaacttatga
     3301 agaagagacg tggaagcagg atatacaaaa caataaaagt ggcagggaca ccattgttgg
     3361 aggttcgtgg acaagttccg gttattgaat ctgcaggatt cgagacagat ttgagattat
     3421 ctacgaatgg tcttggtatg tgtcagctgt acttttggca caagatatgg aggaaggttc
     3481 ctggtgatgt tttggataaa gatgcgttta ttccaaaatt gaaacccgca cctatcaaca
     3541 gtttaagtcg tgatttcgtg atgaaaacaa gaaggcggaa gggtatttct acaggtggat
     3601 ttatgtcaaa tgatggtcct acgcttgaaa agtatataag cgctgaatta tacgctcaat
     3661 taagagaaaa tggcttagta ccgtgaaaat cttggcgcat aagaattaag caatatgtcc
     3721 acaatatttt tattcagcag aatttacata tgacttcgta tttagtgata taactactca
     3781 tcgggactga aaatttttta tttctgccta aatacagatg agatgagctt tatagaattt
     3841 tacgctgtta aaaagttaaa cggtaacaaa gcacagttag ttgaacaacg gtatatagag
     3901 aatggcgagc tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     3961 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4021 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4081 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4141 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4201 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4261 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4321 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4381 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4441 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4501 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     4561 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gatcggcaag
     4621 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga
     4681 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat
     4741 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac
     4801 cagctaacat aaaatgtaag ctttcggggc tctcttgcct tccaacccag tcagaaatcg
     4861 agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg
     4921 aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc
     4981 ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca
     5041 gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat
     5101 tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa
     5161 gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata
     5221 taatacccag caagtcagca tcggaatcta gagcacattc tgcggcctct gtgctctgca
     5281 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
     5341 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
     5401 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttaatcgg caaaaaaaga
     5461 aaagctccgg atcaagattg tacgtaaggt gacaagctat ttttcaataa agaatatctt
     5521 ccactactgc catctggcgt cataactgca aagtacacat atattacgat gctgttctat
     5581 taaatgcttc ctatattata tatatagtaa tgtcgtgatc tatggtgcac tctcagtaca
     5641 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5701 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5761 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5821 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacggatcgc
     5881 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
     5941 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
     6001 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
     6061 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
     6121 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
     6181 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
     6241 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
     6301 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
     6361 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
     6421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6481 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6541 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6661 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6721 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6841 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6901 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7081 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7141 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7201 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7261 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7381 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7561 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7621 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7681 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7741 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7801 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7861 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7921 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7981 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8041 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8101 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8161 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8221 gattctgtgg ataaccgtat taccgccttt ga
//
