LOCUS       sequence-217176-        6203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 503 / 569, Emission: 
            514 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6203)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos-M-ALK1-N-13
FEATURES             Location/Qualifiers
     source          1..6203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2182..2862
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFMYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2984..3105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3021..3040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3075..3094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3112..3567)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3249..3270)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3461..3480
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3594..3698
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3700..4057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3700..3891
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3723..3743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3908..4043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4092..4886
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4092..4886
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4146..4165)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4756..4775
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5074..5093)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5118..5165
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5494..6082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5983..6002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact
      601 cagatctcga gctcaagctt cgaattcacc gccatgacct tgggctcccc caggaaaggc
      661 cttctgatgc tgctgatggc cttggtgacc cagggagacc ctgtgaagcc gtctcggggc
      721 ccgctggtga cctgcacgtg tgagagccca cattgcaagg ggcctacctg ccggggggcc
      781 tggtgcacag tagtgctggt gcgggaggag gggaggcacc cccaggaaca tcggggctgc
      841 gggaacttgc acagggagct ctgcaggggg cgccccaccg agttcgtcaa ccactactgc
      901 tgcgacagcc acctctgcaa ccacaacgtg tccctggtgc tggaggccac ccaacctcct
      961 tcggagcagc cgggaacaga tggccagctg gccctgatcc tgggccccgt gctggccttg
     1021 ctggccctgg tggccctggg tgtcctgggc ctgtggcatg tccgacggag gcaggagaag
     1081 cagcgtggcc tgcacagcga gctgggagag tccagtctca tcctgaaagc atctgagcag
     1141 ggcgacagca tgttggggga cctcctggac agtgactgca ccacagggag tggctcaggg
     1201 ctccccttcc tggtgcagag gacagtggca cggcaggttg ccttggtgga gtgtgtggga
     1261 aaaggccgct atggcgaagt gtggcggggc ttgtggcacg gtgagagtgt ggccgtcaag
     1321 atcttctcct cgagggatga acagtcctgg ttccgggaga ctgagatcta taacacagtg
     1381 ttgctcagac acgacaacat cctaggcttc atcgcctcag acatgacctc ccgcaactcg
     1441 agcacgcagc tgtggctcat cacgcactac cacgagcacg gctccctcta cgactttctg
     1501 cagagacaga cgctggagcc ccatctggct ctgaggctag ctgtgtccgc ggcatgcggc
     1561 ctggcgcacc tgcacgtgga gatcttcggt acacagggca aaccagccat tgcccaccgc
     1621 gacttcaaga gccgcaatgt gctggtcaag agcaacctgc agtgttgcat cgccgacctg
     1681 ggcctggctg tgatgcactc acagggcagc gattacctgg acatcggcaa caacccgaga
     1741 gtgggcacca agcggtacat ggcacccgag gtgctggacg agcagatccg cacggactgc
     1801 tttgagtcct acaagtggac tgacatctgg gcctttggcc tggtgctgtg ggagattgcc
     1861 cgccggacca tcgtgaatgg catcgtggag gactatagac cacccttcta tgatgtggtg
     1921 cccaatgacc ccagctttga ggacatgaag aaggtggtgt gtgtggatca gcagaccccc
     1981 accatcccta accggctggc tgcagacccg gtcctctcag gcctagctca gatgatgcgg
     2041 gagtgctggt acccaaaccc ctctgcccga ctcaccgcgc tgcggatcaa gaagacacta
     2101 caaaaaatta gcaacagtcc agagaagcct aaagggattc aaggcagcgc aggcggaagc
     2161 ggggatccac cggtcgccac catgagtgcg attaagccag acatgaagat caaactccgt
     2221 atggaaggca acgtaaacgg gcaccacttt gtgatcgacg gagatggtac aggcaagcct
     2281 tttgagggaa aacagagtat ggatcttgaa gtcaaagagg gcggacctct gccttttgcc
     2341 tttgatatcc tgaccactgc attcatgtac ggcaacaggg tattcgccaa atatccagac
     2401 aacatacaag actattttaa gcagtcgttt cctaaggggt attcgtggga acgaagcttg
     2461 actttcgaag acgggggcat ttgcattgcc agaaacgaca taacaatgga aggggacact
     2521 ttctataata aagttcgatt ttatggtacc aactttcccg ccaatggtcc agttatgcag
     2581 aagaagacgc tgaaatggga gccctccact gagaaaatgt atgtgcgtga tggagtgctg
     2641 acgggtgata ttcatatggc tttgttgctt gaaggaaatg cccattaccg atgtgacttc
     2701 agaactactt acaaagctaa ggagaagggt gtcaagttac caggctacca ctttgtggac
     2761 cactgcattg agattttaag ccatgacaaa gattacaaca aggttaagct gtatgagcat
     2821 gctgttgctc attctggatt gcctgacaat gccagacgat aagcggccgc gactctagat
     2881 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2941 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3001 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3061 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     3121 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3181 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3241 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3301 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3361 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3421 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3481 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3541 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     3721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3961 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4021 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4081 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4141 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4201 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4261 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4321 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4381 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4441 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4501 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4561 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     4621 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4681 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4741 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4801 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4861 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4921 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4981 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5041 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5101 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5161 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     5221 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5281 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     5341 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5401 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5461 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5521 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5581 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5641 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5701 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5761 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5821 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5881 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5941 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6001 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6061 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6121 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6181 tattaccgcc atgcattagt tat
//
