LOCUS       sequence-217182-       11498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-ATL2
FEATURES             Location/Qualifiers
     source          1..11498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(80..82,83..85,86..796)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(386..405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2578..2602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2633..3221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2686..2706)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3104..3115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3272..3771
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3307..3330)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3704..3723
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3785..4588
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3848..3867)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4458..4477
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             4732..4965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5037..5158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5074..5093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5128..5147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5198..5333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5354..5372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5382..5399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5382..5398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5391..5413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5540..5995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5627..5646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5837..5858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6021..6125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6126..6194,6195..6986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6344..6363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7157..7745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7646..7665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7899..7916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8033..8054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8069..8074,8075..8092,8093..8099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8107..8123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8112..8134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8131..8147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8131..8147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8166..8186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8168..8186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8214..8440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8441..8621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8668..8793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9286..9519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9704..9748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10015..10131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10165..10189
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10320..11498
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10551..11489
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11446..11466
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattagct tggtactaat acgactcact atagggagac ccaagctggc taggtaagct
       61 tggtaccgag ctcggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      121 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      181 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      241 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      301 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      361 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      421 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      481 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      541 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      601 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
      661 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
      721 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
      781 ggacgagctg tacaagggtg gatctggagg ttctggtgga atggcggagg gggacgaggc
      841 agcgcgaggg cagcaaccgc accaggggct gcggcgccgg cgacggacca gcgacccaag
      901 cgccgcggtt aaccacgtct cgtccacgac ctccctaggt gagaattatg aagatgatga
      961 cctagtaaat tctgatgagg ttatgaagaa accatgtcca gtacagattg ttcttgctca
     1021 tgaagatgac cataactttg aacttgatga agaagctttg gagcagatat tgctacagga
     1081 gcacatacga gatcttaaca tagtagtggt atctgtggca ggagcttttc gtaaagggaa
     1141 gtcatttcta ctggacttca tgcttagata catgtataac aaggattctc aaagttggat
     1201 tggtggaaac aatgaaccat tgacaggctt tacatggcga ggtggctgtg aaagagaaac
     1261 aacaggcata caagtttgga atgaagtatt tgtgattgac agacctaatg gaactaaagt
     1321 tgctgtgctg cttatggata cccagggtgc ctttgatagc cagtcaacta tcaaagactg
     1381 tgcaacggtg tttgctctga gcactatgac tagctctgtc caggtatata atctgtctca
     1441 gaatattcaa gaagatgatc ttcaacattt gcaattattt acagagtatg gaagacttgc
     1501 gatggaagaa atctaccaga aaccatttca gacattaatg tttttgattc gagattggag
     1561 ctatccttat gaacattcat atggtttgga aggtggaaag caatttcttg aaaagagatt
     1621 acaggtaaaa caaaatcaac atgaagagct tcagaatgta aggaagcaca tacacaattg
     1681 tttctcaaat cttggttgct tccttttgcc acatcctggt cttaaagttg caactaatcc
     1741 tagttttgat gggagattga aagatattga tgaagacttt aaacgcgagc ttcgaaatct
     1801 ggttccattg ctgcttgccc ctgaaaattt ggtagaaaaa gagataagtg gatctaaagt
     1861 cacttgtaga gatcttgtag aatattttaa ggcttacatc aaaatctatc aaggagaaga
     1921 acttccacat ccaaagtcca tgcttcaggc aacagctgaa gctaataatc ttgctgcagt
     1981 agcaggagca agagatacct attgtaaaag tatggaacag gtatgtggag gggacaagcc
     2041 ttacattgca ccttcagatc tggagcgaaa acacttggat ctcaaggaag tggcgataaa
     2101 acaatttcgt tcagtaaaaa agatgggtgg agatgagttc tgccgtcgtt atcaggacca
     2161 gcttgaagct gaaattgaag aaacctatgc aaattttata aagcacaatg atggcaaaaa
     2221 tatcttctat gctgctcgta ccccagccac actgtttgcg gtcatgtttg ctatgtatat
     2281 aatctcagga ctgactggct tcattggcct aaactctata gctgtcttgt gtaaccttgt
     2341 catggggtta gcactgatat ttctttgtac ttgggcatat gttaaatact ctggggagtt
     2401 cagagaaatt ggaacagtga ttgatcagat tgctgaaaca ctatgggaac aggtattgaa
     2461 gcccctgggt gataatttga tggaggaaaa cataaggcag tctgtaacaa actctatcaa
     2521 agcaggcctg actgaccagg tgtctcatca tgccagatta aagacagact gatctagacc
     2581 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg acaatcaacc
     2641 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2701 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2761 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2821 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2881 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2941 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3001 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     3061 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3121 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3181 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggacctg caggattctg
     3241 cagatatccg gactagtgat ctaattctac cgggtagggg aggcgctttt cccaaggcag
     3301 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     3361 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     3421 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     3481 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     3541 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     3601 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     3661 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     3721 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     3781 caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3841 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3901 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3961 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4021 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4081 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4141 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4201 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4261 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4321 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4381 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4441 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4501 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4561 ctatcgcctt cttgacgagt tcttctgaac gcgtcgcccg ccccacgacc cgcagcgccc
     4621 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga
     4681 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4741 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4801 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4861 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4921 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     4981 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5041 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5101 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5161 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5221 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     5341 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     5401 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     5461 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     5521 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5761 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5821 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5881 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5941 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6001 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6061 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ttgctaatcc
     7381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     8161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     8221 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     8281 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     8341 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     8401 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     8461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8641 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8701 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8761 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8821 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8881 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8941 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     9001 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     9061 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     9121 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     9181 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9241 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9301 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9361 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9421 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9481 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9541 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9601 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9661 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9721 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9781 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9841 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9901 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9961 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
    10021 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    10081 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgtccag
    10141 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc caccatggga
    10201 accgacattg attattgact aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt
    10261 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
    10321 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
    10381 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
    10441 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
    10501 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
    10561 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
    10621 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
    10681 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
    10741 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
    10801 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
    10861 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
    10921 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
    10981 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
    11041 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
    11101 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
    11161 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
    11221 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
    11281 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
    11341 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
    11401 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
    11461 tggttcaaag tttttttctt ccatttcagg tgtcgtga
//
