LOCUS       sequence-21719-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Freeman KW, Gangula RD, Welm BE, Ozen M, Foster BA, Rosen JM, 
            Ittmann M, Greenberg NM, Spencer DM
  TITLE     Conditional activation of fibroblast growth factor receptor (FGFR) 
            1, but not FGFR2, in prostate cancer cells leads to increased 
            osteopontin induction, extracellular signal-regulated kinase 
            activation, and in vivo proliferation.
  JOURNAL   Cancer Res. 2003 Oct 1. 63(19):6237-43.
  PUBMED    14559809
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2003 Oct 1. 63(19):6237-43."
COMMENT     Sequence Label: pSH1/M-FGFR3-Fv-Fvls-E
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..62)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(87..407)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(414..734)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tggatccggt catcaatcag gaattcttag tcgagtgcgt agtctggtac gtcgtacgga
       61 tagtcgactc cggatccacc gccagattcc agttttagaa gctccacatc gaagacgaga
      121 gtggcatgtg gtgggatgat gcctgggtgc ccagtggcac cataggcata atctggagat
      181 atagtcagtt tggctctctg acccacactc atctgggcaa ccccttcttc ccagcctcgg
      241 atcacctcct gcttgcctag cataaactta aagggcttgt ttctgtcccg ggaggaatca
      301 actttctttc catcttcaag catcccggtg tagtgcacca cgcaggtctg gccgcgcttg
      361 gggaaggtgc gcccgtctcc tggggagata gtctccacct gcactccctc gacttccagt
      421 tttagaagct ccacatcgaa gacgagagtg gcatgtggtg ggatgatgcc tgggtgccca
      481 gtggcaccat aggcataatc tggagatata gtcagtttgg ctctctgacc cacactcatc
      541 tgggcaaccc cttcttccca gcctcggatc acctcctgct tgcctagcat aaacttaaag
      601 ggcttgtttc tgtcccggga ggaatcaact ttctttccat cttcaagcat cccggtgtag
      661 tgcaccacgc aggtctggcc gcgcttgggg aaggtgcgcc cgtctcctgg ggagatagtc
      721 tccacctgca ctccctcgac cgtccgangg tcccccgtta ctgggtggac ctggggggta
      781 gcaggtcatg ggtgaacacc gagtcatctc ccggacgagc tgganctagg cg
//
