LOCUS       sequence-217195-        6004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa169 to C-terminus of Flp recombinase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1217..1243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2051..2071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2081..2305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2545..2564)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2610..2665
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2664..2683)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3026..3042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3026..3042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3039..3061)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3050..3066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3074..3080,3081..3098,3099..3104))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3119..3140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3225..3359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3379..3513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3416..3435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3470..3489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3581..3598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3752..4340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3832..3851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4511..5302,5303..5371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5134..5153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5372..5476)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5507..5886
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg cgccaactca agacgagttc acccaactct cccaatcaat
      721 cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc tagctcagcg
      781 aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata ggccacagat
      841 ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc gagtgggatg
      901 cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag agagattaga
      961 tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg aacataggct
     1021 gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag aagaagaaag
     1081 gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta attcggagga
     1141 agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac ttgcttcgat
     1201 cactgaagct atgaactacc catacgatgt tccagattac gcttccggag ggtctggctc
     1261 cggatcaagt ggtggcagcg gtaccagcag gttcaccaag accaagaccc tgtaccagtt
     1321 cctgttcctg gccacattca tcaactgcgg caggttcagc gacatcaaga acgtggaccc
     1381 caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt gcctggtgac
     1441 cgagaccaag acaagcgtgt ccaggcacat ctactttttc agcgccagag gcaggatcga
     1501 ccccctggtg tacctggacg agttcctgag gaacagcgag cccgtgctga agagagtgaa
     1561 caggaccggc aacagcagca gcaacaagca ggagtaccag ctgctgaagg acaacctggt
     1621 gcgcagctac aacaaggccc tgaagaagaa cgccccctac cccatcttcg ctatcaagaa
     1681 cggccctaag agccacatcg gcaggcacct gatgaccagc tttctgagca tgaagggcct
     1741 gaccgagctg acaaacgtgg tgggcaactg gagcgacaag agggcctccg ccgtggccag
     1801 gaccacctac acccaccaga tcaccgccat ccccgaccac tacttcgccc tggtgtccag
     1861 gtactacgcc tacgacccca tcagcaagga gatgatcgcc ctgaaggacg agaccaaccc
     1921 catcgaggag tggcagcaca tcgagcagct gaagggcagc gccgagggca gcatcagata
     1981 ccccgcctgg aacggcatca tcagccagga ggtgctggac tacctgagca gctacatcaa
     2041 caggcggatc cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     2101 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2161 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2221 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2281 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2341 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     2401 agaatccaag cgcggccgcc caggatacat agattaccac aactccgagc ccttccaccg
     2461 ctagcgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2521 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2641 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2701 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     2761 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2821 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2881 atttttttct ttaacatccc taaaattttt cttacatgtt ttactagcca gatttttcct
     2941 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     3001 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3061 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3121 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3181 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3301 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3361 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3421 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3481 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     3541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4021 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4081 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4141 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4201 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4261 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4321 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4381 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4441 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4501 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4561 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4621 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4681 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4741 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4801 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4861 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4921 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4981 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5041 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5101 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5161 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5221 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5281 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5341 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5401 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5461 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca ttgattattg
     5521 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5581 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5641 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     5701 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     5761 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     5821 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     5881 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     5941 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     6001 gggg
//
