LOCUS       sequence-217198-        6223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2270..2290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2300..2524
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2764..2783)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2829..2884
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2883..2902)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3245..3261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3245..3261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3258..3280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3269..3285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3293..3299,3300..3317,3318..3323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3338..3359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3444..3578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3598..3732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3635..3654)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3689..3708
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3800..3817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3971..4559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4051..4070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4730..5521,5522..5590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5353..5372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5591..5695)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5726..6105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga tcgagaacca gctgagcctg ctgggcgact tttctggcgt
      721 gcggcccgac gatgtgaaaa ccgccattca ggccgcccag aaaaagggca tcaacgtggc
      781 cgagaacgag cagttcaagg ccgccttcga gcatctgctg aacgagttca agaagcggga
      841 agagagatac agccccaaca ccctgcggcg gctggaaagc gcctggacct gcttcgtgga
      901 ttggtgcctg gccaaccaca gacacagcct gcctgccacc cccgataccg tggaagcctt
      961 cttcatcgag cgggccgagg aactgcaccg gaacaccctg agcgtgtaca gatgggccat
     1021 cagccgggtg cacagagtgg ccggatgccc tgatccctgc ctggacatct acgtggaaga
     1081 tcggctgaag gccattgccc ggaagaaagt gcgggaaggc gaggccgtga agcaggccag
     1141 ccctttcaac gagcagcatc tgctgaagct gaccagcctg tggtacagaa gctccggagg
     1201 gtctggctcc ggatcaagtg gtggcagcgg taccatggcc gccagcgacg aagtgaacct
     1261 gatcgagagc agaaccgtgg tgcccctgaa cacctgggtg ctgatctcca acttcaaggt
     1321 ggcctacaac atcctgcgga ggcccgacgg caccttcaac agacacctgg ccgagtacct
     1381 ggaccggaaa gtgaccgcca acgccaaccc tgtggacggc gtgttcagct tcgacgtgct
     1441 gatcgaccgg cggatcaacc tgctgagccg ggtgtacaga cccgcctacg ccgatcagga
     1501 acagcccccc tctatcctgg atctggaaaa gcccgtggat ggcgacatcg tgcccgtgat
     1561 cctgttcttc cacggcggca gctttgccca cagcagcgcc aatagcgcca tctacgacac
     1621 cctgtgcaga cggctcgtgg gcctgtgcaa atgcgtggtg gtgtccgtga actaccgcag
     1681 agcccccgag aacccttacc cctgcgccta cgatgatggc tggatcgccc tgaactgggt
     1741 caacagcaga agctggctga agtccaagaa agacagcaag gtgcacatct ttctggccgg
     1801 cgatagcagc ggcggcaata tcgcccataa cgtggccctg agagccggcg agtctggcat
     1861 cgatgtgctg ggcaatatcc tgctgaaccc catgttcggc ggcaacgagc ggaccgagag
     1921 cgagaagtct ctggacggca agtacttcgt gaccgtgcgg gaccgggact ggtactggaa
     1981 ggcctttctg cccgagggcg aggacagaga gcaccccgcc tgcaatccct tcagccccag
     2041 aggcaaaagc ctggaaggcg tgtccttccc aaagtccctg gtggtggtgg ccggcctgga
     2101 cctgatcaga gattggcagc tggcctatgc cgagggcctg aagaaagccg gccaggaagt
     2161 gaagctgatg cacctggaaa aggccaccgt gggcttctac ctgctgccca acaacaacca
     2221 cttccacaac gtgatggacg agatcagcgc cttcgtgaac gccgagtgcc ccaagaaaaa
     2281 gcggaaggtg tgagaattcc tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2341 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2401 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2461 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2521 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2581 agcgtgtacc gctgggagtt cgtagacgga aacaaacgca gaatccaagc gcggccgccc
     2641 aggatacata gattaccaca actccgagcc cttccaccgc tagcgcactc ctcaggtgca
     2701 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2761 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2821 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2881 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2941 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     3001 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     3061 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     3121 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     3181 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     3241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     3421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccagttccg cccattctcc
     3481 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3541 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     3601 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3661 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3721 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5701 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca
     5761 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5821 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5881 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5941 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     6001 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6061 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
     6121 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
     6181 attttttaat tattttgtgc agcgatgggg gcgggggggg ggg
//
