LOCUS       sequence-21721-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Li B, Desai SA, MacCorkle-Chosnek RA, Fan L, Spencer DM
  TITLE     A novel conditional Akt 'survival switch' reversibly protects cells 
            from apoptosis.
  JOURNAL   Gene Ther. 2002 Feb . 9(4):233-44.
  PUBMED    11896462
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Ther. 
            2002 Feb . 9(4):233-44."
COMMENT     Sequence Label: pSH1/M-FRBl2-E
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..42)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(595..636)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaattcttag tcgagtgcgt agtctggtac gtcgtacgga tagtcgacct ttgagattcg
       61 tcggaacaca tgataataga ggtcccaggc ttggaggagg tccttgacat tccctgattt
      121 catgtacttc ctgcaccact cttgggcctc cattaaatct cgaccatagg cctgattaaa
      181 ggatgtttcc ttcagagtct gggggccccg ttccatcata gcatgcaagg gctccagcac
      241 ctcaaacatg cctttcacgt tcctttcccc aaagtacaaa cgagatgcct cttccaggcc
      301 ttcatgccac atctcctcga cctttgagat tcgtcggaac acatgataat agaggtccca
      361 ggcttggagg aggtccttga cattccctga tttcatgtac ttcctgcacc actcttgggc
      421 ctccattaaa tctcgaccat aggcctgatt aaaggatgtt tccttcagag tctgggggcc
      481 ccgttccatc atagcatgca agggctccag cacctcaaac atgcctttca cgttcctttc
      541 cccaaagtac aaacgagatg cctcttccag gccttcatgc cacatctcct cgaggcgctg
      601 gctggggtcc ttaggcttgc tcttgctact ccccatggtg gccgcggccg ctctagccct
      661 cgatcgaggg ctagagcagc ttttagagca gaagtaacac ttccgtaca
//
