LOCUS       sequence-217210-        6119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6119)
  TITLE     plasmids for expression of yeast spliceosomal proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-Slu7
FEATURES             Location/Qualifiers
     source          1..6119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(22..27,28..43,44..50)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    58..74
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             97..750
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     708..730
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             757..780
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(2008..2030)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2175..2193)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2261..2365
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2366..2434,2435..3226)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2584..2603)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3397..3985
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3886..3905
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4139..4156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4229..4306
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4307..5389
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4326..4345)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5438..5443,5444..5461,5462..5468)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5476..5492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5500..5516
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5500..5516
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5512..6039
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(5532..5549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5532..5548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5541..5563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg
       61 tgagcggata acaatttcac acaggaaaca gtattcatgt cccctatact aggttattgg
      121 aaaattaagg gccttgtgca acccactcga cttcttttgg aatatcttga agaaaaatat
      181 gaagagcatt tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg
      241 ggtttggagt ttcccaatct tccttattat attgatggtg atgttaaatt aacacagtct
      301 atggccatca tacgttatat agctgacaag cacaacatgt tgggtggttg tccaaaagag
      361 cgtgcagaga tttcaatgct tgaaggagcg gttttggata ttagatacgg tgtttcgaga
      421 attgcatata gtaaagactt tgaaactctc aaagttgatt ttcttagcaa gctacctgaa
      481 atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat atttaaatgg tgatcatgta
      541 acccatcctg acttcatgtt gtatgacgct cttgatgttg ttttatacat ggacccaatg
      601 tgcctggatg cgttcccaaa attagtttgt tttaaaaaac gtattgaagc tatcccacaa
      661 attgataagt acttgaaatc cagcaagtat atagcatggc ctttgcaggg ctggcaagcc
      721 acgtttggtg gtggcgacca tcctccaaaa tcggatctgg aagttctgtt ccaggggccc
      781 ctgggatccc cggaattcat gaataataac agcagaaaca acgaaaatcg aagcactatt
      841 aacagaaata aaaggcaact acaacaagca aaagaaaaaa atgaaaatat tcatatcccc
      901 aggtatatta gaaatcaacc atggtactat aaggataccc ccaaagaaca agaagggaag
      961 aagcccggca atgatgatac gagcactgca gaaggaggag aaaaaagcga ctacttggtg
     1021 catcataggc aaaaagcaaa agggggtgct ttagatattg acaataattc agaaccaaaa
     1081 attggtatgg gtataaagga tgagttcaaa ctaatcagac cccagaagat gtccgtccga
     1141 gattctcatt cgctgtcatt ttgtaggaat tgtggggaag cagggcataa ggagaaagac
     1201 tgcatggaaa aacctcgtaa gatgcagaag cttgttcccg atttaaattc acaaaaaaat
     1261 aatggcacag ttttagtacg agctactgat gatgactggg actccagaaa agatagatgg
     1321 tacggttact cagggaaaga atacaatgaa ctgataagta agtgggagcg tgataaaaga
     1381 aataaaataa aaggaaaaga caaatcccaa actgatgaaa cactatggga tacagatgaa
     1441 gagatagaac taatgaagtt agaactttac aaggattccg taggttcatt gaagaaagat
     1501 gatgctgata attctcagtt gtataggaca tcaacgagat tgagagaaga taaggctgct
     1561 tacttgaacg acataaattc aacggagagt aattatgatc ctaaatcaag attgtacaaa
     1621 actgaaacac tgggcgcagt tgatgaaaaa tcaaaaatgt tccgcagaca tttgacaggt
     1681 gaaggcctaa aattaaacga attgaaccag tttgctagat ctcacgctaa ggaaatgggt
     1741 atacgtgatg aaattgagga taaggaaaaa gtacaacatg ttttagtcgc caatcctact
     1801 aaatatgaat atctgaagaa aaaacgggaa caagaagaaa ccaagcagcc caagattgtc
     1861 agcattggag atctggaagc taggaaagta gatggtacaa agcaatctga ggaacaacgg
     1921 aaccacttaa aagatttata tggttgagcg gccgcatcgt gactgactga cgatctgcct
     1981 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2041 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2101 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtataattc
     2161 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2221 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2581 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     2641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2881 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     2941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3541 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4021 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4081 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4141 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4201 gtgcggtatt tcacaccgca taaattccga caccatcgaa tggcgcaaaa cctttcgcgg
     4261 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
     4321 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     4381 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     4441 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     4501 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     4561 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     4621 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     4681 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     4741 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     4801 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     4861 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     4921 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     4981 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     5041 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     5101 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     5161 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     5221 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     5281 aagaaaaacc acgctagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5341 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     5401 ttaatgtaag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     5461 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     5521 attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5581 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5641 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg
     5701 tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat
     5761 actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac
     5821 gtgacctatc ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt
     5881 tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt
     5941 tttgatggcg ttggaattag cttatcgact gcacggtgca ccaatgcttc tggcgtcagg
     6001 cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg
     6061 ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctgg
//
