LOCUS       sequence-21724-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: pActPL-VP16AD
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..69
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             82..315
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 cgatctgcgg ccgcaaacat ggataaagcg gaattaattc ccgagcctcc aaaaaagaag
       61 agaaaggtcg aattgggtac cgcccccccg accgatgtca gcctggggga cgagctccac
      121 ttagacggcg aggacgtggc gatggcgcat gccgacgcgc tagacgattt cgatctggac
      181 atgttggggg acggggattc cccgggtccg ggatttaccc cccacgactc cgccccctac
      241 ggcgctctgg atatggccga cttcgagttt gagcagatgt ttaccgatgc ccttggaatt
      301 gacgagtacg gtggggctag cggaggaggt ggtggaggtg gaggtggagg tactagtctg
      361 gagatcgagg ccgccttcct ggagcgcgag aacaccgccc tggagacccg cgtggccgag
      421 ctgcgccagc gcgtgcagcg cctgcgcaac cgcgtgagcc agaaccgcac ccgctacggc
      481 cccctgggcg gcggcaagta aggcgcgcca taaccgatcc gtcgaccatg aagatcaaga
      541 tcattgcccc gccagagcgc aagtactctg tctggatcgg
//
