LOCUS       sequence-217242-       10586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  prp8-101.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Umen JG, Guthrie C
  TITLE     Mutagenesis of the yeast gene PRP8 reveals domains governing the 
            specificity and fidelity of 3' splice site selection.
  JOURNAL   Genetics. 1996 Jun;143(2):723-39.
  PUBMED    8725222
REFERENCE   2  (bases 1 to 10586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "1996-06"; volume: "143(2)"; pages: "723-39"
COMMENT     Sequence Label: pRS303 prp8-101
FEATURES             Location/Qualifiers
     source          1..10586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..61
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    178..199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(214..219,220..237,238..244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    252..268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     257..279
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     276..292
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     276..292
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        313..331
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6592..6611)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6593..6611)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6618..6635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6618..6634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6627..6649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6775..7230
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6862..6881)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7072..7093
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7490..8152)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(8153..8340)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(8487..8506)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8606..8628
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8666..8684)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8752..8856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8857..8925,8926..9717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9075..9094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9888..10476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10377..10396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
       61 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      121 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      181 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      241 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      301 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg gccccccctc
      361 gaggtcgaca aggacacaat atgttaaatt tattaatcca cagaaagggc ctaacttatt
      421 tacatctcga ctacaatttc aatttaaaac caactaaaac cttaaccaca aaagaacgta
      481 aaaagtcaag acttggaaac tcatttcatt tgatgcgtga attgctaaaa atgatgaaac
      541 tgattgtaga tacacatgtg cagtttaggt taggaaatgt agatgccttc cagttagcag
      601 atggtataca ttatattttg aaccacatag gacaattgac cggtatttat cgttataaat
      661 ataaagtaat gcatcaaatc cgcgcatgta aagatctgaa acatatcata tattataagt
      721 ttaataaaaa tttgggaaaa ggacctggtt gtggattttg gcaacctgcg tggagagtgt
      781 ggctaaattt cttaagaggc actataccac tactcgaaag atatattgga aaccttatca
      841 cacgtcaatt tgaaggccgt tctaatgaaa ttgtcaaaac tacaactaaa caaagacttg
      901 acgcctacta cgatcttgag ttacgaaact cggtcatgga tgacatttta gaaatgatgc
      961 ctgagagtat tcgacagaaa aaggcaagaa ctatactgca acatttaagt gaggcttgga
     1021 gatgttggaa ggctaatatt ccatgggatg ttcctggtat gccagcccca atcaaaaaaa
     1081 taattgaacg gtatataaaa tccaaagctg atgcctgggt atctgcagct cattacaatc
     1141 gagaacgtat aaagcgtgga gctcacgtcg aaaagactat ggttaaaaaa aatctggggc
     1201 gtttaacgag actttggatc aaaaatgagc aggaacgtca aaggcaaata cagaaaaacg
     1261 gtcccgaaat tactcccgaa gaggctacga ccatattttc tgttatggtt gagtggcttg
     1321 aatcaaggag cttttcacca ataccatttc cacctttaac ttacaaaaat gatactaaga
     1381 tattagttct tgccctcgaa gacctaaaag atgtctacgc atcaaaagta cgtttaaatg
     1441 catctgaaag ggaagaactt gcgttgatag aggaggctta tgataatcct cacgatactt
     1501 tgaacagaat caaaaagtac ttgttgaccc agcgagtttt taagcctgtt gatataacca
     1561 tgatggaaaa ctatcaaaac atttcccctg tttattcagt tgatcccttg gaaaagatta
     1621 ccgatgcata tcttgatcag tatttatggt acgaagctga ccaacgaaag cttttcccaa
     1681 actggattaa accaagtgat tcagagatac cacctcttct ggtatacaag tggactcagg
     1741 gtataaacaa cctatctgaa atttgggatg tatccagagg ccaatctgca gttttgcttg
     1801 agactacttt gggtgaaatg gccgaaaaaa ttgactttac tttacttaat agattacttc
     1861 gcctgattgt agatcctaat attgctgact atatcactgc aaaaaataat gttgttatca
     1921 actttaaaga tatgagtcac gtcaacaaat atggcttaat acgcgggttg aagttcgctt
     1981 ctttcatatt ccaatattac ggactagtta tagatctttt actattgggt caggaaaggg
     2041 ctacagattt ggctggtcca gctaacaatc caaatgaatt tatgcaattc aagagcaaag
     2101 aagtagaaaa ggcacatccg atcagacttt acaccagata tttagatcgt atatatatgc
     2161 tttttcactt tgaagaagat gagggagagg aattgactga tgaatacttg gcagagaatc
     2221 cagatccaaa ctttgaaaac agcatcggat acaataatag aaaatgctgg cccaaagatt
     2281 cgcgcatgag gctaatacgt caggacgtta acctagggcg agcagtgttt tgggaaattc
     2341 aaagtagggt gccaacttcg ttaacgtcaa ttaaatggga aaatgctttt gtttccgtat
     2401 acagtaaaaa caatccgaac ttactcttct ctatgtgtgg ttttgaagtc agaatattac
     2461 caagacaaag aatggaagag gtggtttcta acgatgaagg tgtttgggat ttagttgacg
     2521 aaagaaccaa gcaaagaact gcaaaagcat atttaaaggt atctgaagaa gaaatcaaaa
     2581 aatttgacag cagaataaga ggcatattga tggcctccgg ttcaaccact tttactaaag
     2641 tggccgctaa atggaacact tcactgatat cccttttcac atactttaga gaagccattg
     2701 ttgccacgga gccgttatta gatatcctgg tgaaaggaga aacaagaatt caaaaccgtg
     2761 tcaaacttgg tttaaattcg aaaatgccaa caagatttcc acctgctgtt ttttatacac
     2821 caaaagaact tggtgggttg ggtatgatta gtgcttctca tattttaatt cctgcatctg
     2881 acttaagttg gtcaaaacag accgatactg gaattacgca ttttcgtgct ggtatgacgc
     2941 acgaggatga aaaattaatc ccaactatct tccgttacat cactacttgg gaaaatgaat
     3001 ttttagactc tcaaagggtg tgggccgagt atgcaacgaa aaggcaggaa gctattcagc
     3061 agaataggag gttagctttt gaagaacttg aaggatcgtg ggatcgtggt atacctcgta
     3121 ttagcacttt atttcaaaga gatcgacata ctctagcgta tgacagaggt cacagaatcc
     3181 gtagggaatt taaacagtac tctctagaga gaaacagccc cttttggtgg acaaactccc
     3241 accatgacgg taaattatgg aatttaaatg cctatagaac tgatgtgatt caagccctag
     3301 gaggtattga aacaattttg gaacacactt tatttaaggg gactggtttt aattcatggg
     3361 aaggtttgtt ttgggaaaag gcgtccgggt ttgaggattc catgcaattt aagaagctaa
     3421 ctcatgctca aagaactggt ttaagccaaa ttccaaatcg tagatttacc ttatggtggt
     3481 caccaactat taacagagca aatgtctatg tcggtttctt ggtgcaattg gatctgactg
     3541 gtatcttcct ccatggtaaa attcctacgt taaaaatatc tttaattcaa atattccgtg
     3601 ctcatttgtg gcaaaaaata catgaaagta tagtttttga tatttgtcaa attctcgatg
     3661 gtgagctaga tgttttgcaa attgaatctg tgactaaaga aacagtgcat ccccgcaaat
     3721 cgtacaagat gaattcatct gccgctgaca taactatgga aagtgttcat gaatgggaag
     3781 tttcaaaacc ctccctatta catgaaacaa atgactcatt taagggattg attaccaaca
     3841 aaatgtggtt tgatgtacaa ttgagatacg gtgattatga ttctcatgat atatctcgtt
     3901 atgtgagggc aaaattcctc gattatacaa cagataatgt cagcatgtat ccctccccaa
     3961 caggtgttat gatcggtatc gatttggctt ataacatgta tgatgcctat ggtaattggt
     4021 tcaacggatt aaaacctttg atacaaaata gtatgagaac aattatgaaa gctaacccag
     4081 cattatatgt ccttcgtgaa cgtattagaa aaggtcttca aatatatcaa tctagtgtac
     4141 aggaaccatt tttaaactcc tcaaactatg ccgagttatt caataacgac attaaactct
     4201 tcgttgacga cactaatgtg tatagagtta ctgtccacaa gacttttgaa gggaacgttg
     4261 ctacaaaagc aattaatggt tgcattttca ctttaaatcc aaagactggg catctgtttc
     4321 tgaaaattat ccatacctct gtatgggccg gtcaaaaacg tttaagccaa ttggccaaat
     4381 ggaaaacagc tgaggaagtt agtgctcttg tgagatcttt gcccaaagaa gagcagccaa
     4441 aacaaattat cgtcacaaga aaggcaatgc ttgatccttt agaggttcac atgcttgact
     4501 ttcctaacat agcaatcagg ccaacaaagc tgcgactacc attttcagct gcaatgtcaa
     4561 tagataaact ttctgatgtt gtgatgaaag ctactgaacc tcagatggtt ctgtttaaca
     4621 tttatgatga ttggttagac cgtatttcat cgtacactgc attttctaga cttacactac
     4681 ttttaagagc tttaaagacg aacgaggaaa gtgccaaaat gattctactc agtgacccca
     4741 cgatcactat caaatcgtac catttatggc cttcattcac tgatgaacaa tggatcacta
     4801 tcgaatctca aatgagagat ttgattttga ctgagtacgg cagaaaatac aatgtcaata
     4861 tatctgcctt aactcaaacg gaaattaaag atattatatt gggtcaaaat atcaaggcac
     4921 catctgtgaa gagacaaaaa atggctgaat tagaagctgc caggtcagaa aagcagaacg
     4981 acgaagaagc tgcaggtgca tcaactgtta tgaaaactaa aactatcaat gcgcaaggtg
     5041 aggagatagt tgtagtagct tccgctgatt acgaaagtca aacgtttagt tcaaagaatg
     5101 aatggagaaa atctgcaatt gctaatacac tattatattt gaggttaaaa aacatttatg
     5161 tttcggctga tgatttcgtg gaggaacaga atgtttacgt ccttccaaaa aatttattaa
     5221 agaagtttat tgagatatcc gatgtgaaaa tccaggtggc ggcctttatt tatggtatgt
     5281 cagcaaaaga tcatccgaag gtgaaagaaa tcaagactgt tgtgctggta cctcaattag
     5341 gccacgtagg atccgttcag ataagcaaca tccctgacat cggagacctt ccggatactg
     5401 aaggccttga actactcggt tggatccata cccagacaga agaacttaaa ttcatggctg
     5461 cgtctgaagt agcaactcat tctaaacttt ttgcggataa gaaaagagac tgcattgata
     5521 tttcaatttt ttccaccccg ggatcagtat cattaagcgc ttataacctg accgacgaag
     5581 gttaccaatg gggtgaagaa aataaggata taatgaacgt gctatcggaa gggtttgagc
     5641 ctactttcag cacgcatgcc caattacttc tctctgaccg tatcacaggt aattttataa
     5701 ttccatctgg aaacgtttgg aactacacat ttatgggaac agcatttaac caagaaggtg
     5761 actacaattt taaatacggc attcctttgg aattctataa tgagatgcat cgtccagtac
     5821 actttttaca gtttagcgaa ttggcggggg acgaagagtt agaggccgaa caaatcgatg
     5881 tatttagctg aatatattcc ccaataaact ggaatctgtt atttcataga tatatatgta
     5941 agtataagtt tgtatgtata atattcaacg cattttttat gcccgtcgta gttgctacct
     6001 accacacgat gatgtattat aatttcggaa ctacttcaaa gctacgcttt ccgcactatg
     6061 aaaagctata agtatgtgaa aattggtcat tgactgtgat tctatttcaa aaagtaacca
     6121 aaggagatag acatcgttcg gacagaaaat ggtccgttgt cactcccatt ccattatgtc
     6181 cttcatatag aaattggata ttcttgtttt catttcgcgc aataaagcaa ttccgtgcgg
     6241 cagaaagaac ttatatatat ttaactgatc ttacgctatt tatggcaaaa cttgtgttac
     6301 atttttgaag ataaagttac aatcattacg gcagcctcaa aacaaaattg ggagaaaaca
     6361 tactcaagtg agtactcatt ttgtgcaagc aaacactgac aattgaagag atccacagga
     6421 cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga
     6481 ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca
     6541 tcaacgcata tagcgctaga gcggccgcca ccgcggtgga gctccaattc gccctatagt
     6601 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     6781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7201 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     7261 gtgcggtatt tcacaccgca tagatccgtc gagttcaaga gaaaaaaaaa gaaaaagcaa
     7321 aaagaaaaaa ggaaagcgcg cctcgttcag aatgacacgt atagaatgat gcattacctt
     7381 gtcatcttca gtatcatact gttcgtatac atacttactg acattcatag gtatacatat
     7441 atacacatgt atatatatcg tatgctgcag ctttaaataa tcggtgtcac tacataagaa
     7501 cacctttggt ggagggaaca tcgttggtac cattgggcga ggtggcttct cttatggcaa
     7561 ccgcaagagc cttgaacgca ctctcactac ggtgatgatc attcttgcct cgcagacaat
     7621 caacgtggag ggtaattctg ctagcctctg caaagctttc aagaaaatgc gggatcatct
     7681 cgcaagagag atctcctact ttctcccttt gcaaaccaag ttcgacaact gcgtacggcc
     7741 tgttcgaaag atctaccacc gctctggaaa gtgcctcatc caaaggcgca aatcctgatc
     7801 caaacctttt tactccacgc acggccccta gggcctcttt aaaagcttga ccgagagcaa
     7861 tcccgcagtc ttcagtggtg tgatggtcgt ctatgtgtaa gtcaccaatg cactcaacga
     7921 ttagcgacca gccggaatgc ttggccagag catgtatcat atggtccaga aaccctatac
     7981 ctgtgtggac gttaatcact tgcgattgtg tggcctgttc tgctactgct tctgcctctt
     8041 tttctgggaa gatcgagtgc tctatcgcta ggggaccacc ctttaaagag atcgcaatct
     8101 gaatcttggt ttcatttgta atacgcttta ctagggcttt ctgctctgtc atctttgcct
     8161 tcgtttatct tgcctgctca ttttttagta tattcttcga agaaatcaca ttactttata
     8221 taatgtataa ttcattatgt gataatgcca atcgctaaga aaaaaaaaga gtcatccgct
     8281 aggtggaaaa aaaaaaatga aaatcattac cgaggcataa aaaaatatag agtgtactag
     8341 aggaggccaa gagtaataga aaaagaaaat tgcgggaaag gactgtgtta tgacttccct
     8401 gactaatgcc gtgttcaaac gatacctggc agtgactcct agcgctcacc aagctcttaa
     8461 aacgggaatt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8521 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8581 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     8641 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8701 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8761 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     8821 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8881 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8941 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9001 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9061 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9121 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9181 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9241 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9301 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9361 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9421 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9481 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9541 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9601 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9661 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9721 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9781 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9841 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9901 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9961 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10021 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10081 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10141 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10201 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10261 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10321 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10381 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10441 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10501 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10561 tctgtggata accgtattac cgcctt
//
