LOCUS       sequence-217248-        8218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Graff, Mock
  TITLE     Mock lab P. tricornutum plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAGM4723:Ble:Cas9
FEATURES             Location/Qualifiers
     source          1..8218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    14..38
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             374..748
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     CDS             1265..5368
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1265..5368
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1265..5368
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5381..5401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5408..5410,5411..5413,5414..6127)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5453..5474)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5714..5733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6061..6082
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6438..6542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6543..7337
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7508..8096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7997..8016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
       61 ataacacatt gcggacgttt ttaatgtact ggggttgaac actctgtgcc gaattcggat
      121 ccggagacat accttcagcg tcgtctacac tgtcacagtc aactgacagt aatcgttgat
      181 ccggagagat tcaaaattca atctgtttgg acctggataa gacacaagag cgacatcctg
      241 acatgaacgc cgtaaacagc aaatcctggt tgaacacgta tccttttggg ggcctcccgc
      301 tacgacgctc gctccagctg gggcttcctt actatacaca gcgcgcatat ttcacggttg
      361 ccagatgtca agaatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
      421 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
      481 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
      541 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
      601 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
      661 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
      721 gcacttcgtg gccgaggagc aggactgagc ttccttcctt aaaaatttaa ttttcattag
      781 ttgcagtcac tccgctttgg tttcacagtc aggaataaca ctagctcgac ttcaccatgg
      841 atgccaatct cgcctattca tggtgtataa aagttcaaca tccaaagcta gaacttttgg
      901 aaagagaaag aatatccgaa tagggcacgg cgtgccgtat tgttggagtg gactagcaga
      961 aagtgaggaa ggcacaggat gagttttctc gagcgctgca agaattcaag cttggagaca
     1021 taccttcagc gtcgtctaca ctgtcacagt caactgacag taatcgttga tccggagaga
     1081 ttcaaaattc aatctgtttg gacctggata agacacaaga gcgacatcct gacatgaacg
     1141 ccgtaaacag caaatcctgg ttgaacacgt atccttttgg gggcctcccg ctacgacgct
     1201 cgctccagct ggggcttcct tactatacac agcgcgcata tttcacggtt gccagatgtc
     1261 aagaatggac aagaagtact ccattgggct cgatatcggc acaaacagcg tcggctgggc
     1321 cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga
     1381 tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc
     1441 cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat
     1501 ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca
     1561 taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt
     1621 tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat atcatctgag
     1681 gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc tcgcgctggc
     1741 gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc cagacaacag
     1801 cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt tcgaagagaa
     1861 cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc tgtccaaatc
     1921 ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg gcctgtttgg
     1981 taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact tcgacctggc
     2041 cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg acaatctgct
     2101 ggcccagatc ggcgaccagt acgcagacct ttttttggcg gcaaagaacc tgtcagacgc
     2161 cattctgctg agtgatattc tgcgagtgaa cacggagatc accaaagctc cgctgagcgc
     2221 tagtatgatc aagcgctatg atgagcacca ccaagacttg actttgctga aggcccttgt
     2281 cagacagcaa ctgcctgaga agtacaagga aattttcttc gatcagtcta aaaatggcta
     2341 cgccggatac attgacggcg gagcaagcca ggaggaattt tacaaattta ttaagcccat
     2401 cttggaaaaa atggacggca ccgaggagct gctggtaaag cttaacagag aagatctgtt
     2461 gcgcaaacag cgcactttcg acaatggaag catcccccac cagattcacc tgggcgaact
     2521 gcacgctatc ctcaggcggc aagaggattt ctaccccttt ttgaaagata acagggaaaa
     2581 gattgagaaa atcctcacat ttcggatacc ctactatgta ggccccctcg cccggggaaa
     2641 ttccagattc gcgtggatga ctcgcaaatc agaagagact atcactccct ggaacttcga
     2701 ggaagtcgtg gataaggggg cctctgccca gtccttcatc gaaaggatga ctaactttga
     2761 taaaaatctg cctaacgaaa aggtgcttcc taaacactct ctgctgtacg agtacttcac
     2821 agtttataac gagctcacca aggtcaaata cgtcacagaa gggatgagaa agccagcatt
     2881 cctgtctgga gagcagaaga aagctatcgt ggacctcctc ttcaagacga accggaaagt
     2941 taccgtgaaa cagctcaaag aagattattt caaaaagatt gaatgtttcg actctgttga
     3001 aatcagcgga gtggaggatc gcttcaacgc atccctggga acgtatcacg atctcctgaa
     3061 aatcattaaa gacaaggact tcctggacaa tgaggagaac gaggacattc ttgaggacat
     3121 tgtcctcacc cttacgttgt ttgaagatag ggagatgatt gaagaacgct tgaaaactta
     3181 cgctcatctc ttcgacgaca aagtcatgaa acagctcaag aggcgccgat atacaggatg
     3241 ggggcggctg tcaagaaaac tgatcaatgg gatccgagac aagcagagtg gaaagacaat
     3301 cctggatttt cttaagtccg atggatttgc caaccggaac ttcatgcagt tgatccatga
     3361 tgactctctc acctttaagg aggacatcca gaaagcacaa gtttctggcc agggggacag
     3421 tctccacgag cacatcgcta atcttgcagg tagcccagct atcaaaaagg gaatactgca
     3481 gaccgttaag gtcgtggatg aactcgtcaa agtaatggga aggcataagc ccgagaatat
     3541 cgttatcgag atggcccgag agaaccaaac tacccagaag ggacagaaga acagtaggga
     3601 aaggatgaag aggattgaag agggtataaa agaactgggg tcccaaatcc ttaaggaaca
     3661 cccagttgaa aacacccagc ttcagaatga gaagctctac ctgtactacc tgcagaacgg
     3721 cagggacatg tacgtggatc aggaactgga catcaatcgg ctctccgact acgacgtgga
     3781 tcatatcgtg ccccagtctt ttctcaaaga tgattctatt gataataaag tgttgacaag
     3841 atccgataaa aatagaggga agagtgataa cgtcccctca gaagaagttg tcaagaaaat
     3901 gaaaaattat tggcggcagc tgctgaacgc caaactgatc acacaacgga agttcgataa
     3961 tctgactaag gctgaacgag gtggcctgtc tgagttggat aaagccggct tcatcaaaag
     4021 gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc caaattctcg attcacgcat
     4081 gaacaccaag tacgatgaaa atgacaaact gattcgagag gtgaaagtta ttactctgaa
     4141 gtctaagctg gtttcagatt tcagaaagga ctttcagttt tataaggtga gagagatcaa
     4201 caattaccac catgcgcatg atgcctacct gaatgcagtg gtaggcactg cacttatcaa
     4261 aaaatatccc aagcttgaat ctgaatttgt ttacggagac tataaagtgt acgatgttag
     4321 gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc accgctaagt acttctttta
     4381 cagcaatatt atgaattttt tcaagaccga gattacactg gccaatggag agattcggaa
     4441 gcgaccactt atcgaaacaa acggagaaac aggagaaatc gtgtgggaca agggtaggga
     4501 tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg aacatcgtta aaaagaccga
     4561 agtacagacc ggaggcttct ccaaggaaag tatcctcccg aaaaggaaca gcgacaagct
     4621 gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc ggattcgatt ctcctacagt
     4681 cgcttacagt gtactggttg tggccaaagt ggagaaaggg aagtctaaaa aactcaaaag
     4741 cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca agcttcgaaa aaaaccccat
     4801 cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa gacctcatca ttaagcttcc
     4861 caagtactct ctctttgagc ttgaaaacgg ccggaaacga atgctcgcta gtgcgggcga
     4921 gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac gttaatttct tgtatctggc
     4981 cagccactat gaaaagctca aaggatctcc cgaagataat gagcagaagc agctgttcgt
     5041 ggaacaacac aaacactacc ttgatgagat catcgagcaa ataagcgaat tctccaaaag
     5101 agtgatcctc gccgacgcta acctcgataa ggtgctttct gcttacaata agcacaggga
     5161 taagcccatc agggagcagg cagaaaacat tatccacttg tttactctga ccaacttggg
     5221 cgcgcctgca gccttcaagt acttcgacac caccatagac agaaagcggt acacctctac
     5281 aaaggaggtc ctggacgcca cactgattca tcagtcaatt acggggctct atgaaacaag
     5341 aatcgacctc tctcagctcg gtggagacag cagggctgac cccaagaaga agaggaaggt
     5401 gggttcgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5461 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5521 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5581 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
     5641 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5701 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5761 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5821 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5881 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5941 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6001 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6061 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6121 caagtgagct tccttcctta aaaatttaat tttcattagt tgcagtcact ccgctttggt
     6181 ttcacagtca ggaataacac tagctcgact tcaccatgga tgccaatctc gcctattcat
     6241 ggtgtataaa agttcaacat ccaaagctag aacttttgga aagagaaaga atatccgaat
     6301 agggcacggc gtgccgtatt gttggagtgg actagcagaa agtgaggaag gcacaggatg
     6361 agttttctcg agcgctacta gaggatgcac atgtgaccga gggacacgaa gtgatcccca
     6421 catcaaggct ccgagtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6541 gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
     6601 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
     6661 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     6721 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     6781 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
     6841 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     6901 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     6961 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     7021 aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     7081 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     7141 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
     7201 acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc
     7261 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
     7321 tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag attgatttaa
     7381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8101 ccagcaacgc ggccttttta cggttcctgc tcggatctgt tggaccggac agtagtcatg
     8161 gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagct
//
