LOCUS       sequence-21727-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Luan H, Peabody NC, Vinson CR, White BH
  TITLE     Refined spatial manipulation of neuronal function by combinatorial 
            restriction of transgene expression.
  JOURNAL   Neuron. 2006 Nov 9. 52(3):425-36.
  PUBMED    17088209
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Nov 9. 52(3):425-36."
COMMENT     Sequence Label: pCaST-elav-Gal4AD
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ataccccacc aaacccaaaa aaagagatct ttaatacgac tcactatagg gcgagcgccg
       61 ccatggagta cccatacgac gtaccagatt acgctggagg aggtggtgga ggtggaggtg
      121 gaggtactag tctggagatc gaggccgcct tcctggagcg cgagaacacc gccctggaga
      181 cccgcgtggc cgagctgcgc cagcgcgtgc agcgcctgcg caaccgcgtg agccagaacc
      241 gcacccgcta cggccccctg ggcggcggca agtaaggcgc gcctctagga tccaaatgca
      301 attggctaaa aaccgaaacc aaaacgcaac aaagtatacg aaacacttgt gaaaccgtac
      361 aaacaattgt ggaaaaaaaa ttaaaagatt attaagattg aagtctcaat aaacattagt
      421 gcttaaataa acttaaagcg acccgcgtgg agagtgcagt aaaaagaata acttttgaaa
      481 taaatattta ccaaacagaa aaatatttta taaatattta aataagtgaa aaacaaattg
      541 gttactctga aacaaagaat attcaaattg gcgctaaaac aaaggagaaa aaatttcaag
      601 aatattatac agataataag acatatttaa ctatataaaa ccaaacttaa tcaacaaaga
      661 acaaggagtg aaaaaaata
//
